Differential stress-response expression of two flavonol synthase genes and accumulation of flavonols in tartary buckwheat

Li, Xiaohua; Kim, Yeon Bok; Kim, Yeji; Zhao, Shicheng; Kim, Haeng Hoon; Chung, Eui-Young; Lee, Jai‐Heon; Park, Sang Un

doi:10.1016/j.jplph.2013.06.010

Cited by 61 publications

(50 citation statements)

References 53 publications

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“…fertilizers) or exposure to abiotic stresses (e.g. drought and salinity) (Li et al, 2013;Xiang et al, 2013;Zhao et al, 2013). Flour mainly consists of the embryo and endosperm, both of which can accumulate flavonoids during seed development.…”

Section: Discussionmentioning

confidence: 99%

Changes in seed growth, levels and distribution of flavonoids during tartary buckwheat seed development

Song

Xiang

Lin

et al. 2016

Plant Production Science

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We investigated the change in seed shape and weight as well as the accumulation and distribution of anthocyanins, melanin, and flavonoids during tartary buckwheat seed development. Pericarp dry weight increased slowly while the increase in flour dry weight was much greater. Increases in the dry weight of the embryo and endosperm were associated with change in seed shape. The highest anthocyanin content was observed in stage two while the highest melanin content occurred in stage five. There was a considerable decrease in total flavonoids, rutin, quercetin and kaempferol in mature seeds relative to seeds that were still developing. Additionally, there were significant correlations (correlation coefficient > .900, p < .05) among the changes in rutin, quercetin and kaempferol between flour and the pericarp. In mature seeds, 95% of the flavonoids were present in flour.

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Section: Discussionmentioning

confidence: 99%

Changes in seed growth, levels and distribution of flavonoids during tartary buckwheat seed development

Song

Xiang

Lin

et al. 2016

Plant Production Science

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“…Thus, we suggested that between transcript levels involved in anthocyanin biosynthesis and anthocyanin accumulation have correlation in the red-flowered buckwheat flowers. 34) In F. tataricum Hokkai T10 and Hokkai T8 cultivars, Li et al 35) described that flavonol synthase1 showed the highest transcript levels in the flowers (mixture of flowering stages) than in any other organ (e.g., leaves, stems or roots) and amount of quercetin and kaemperol were significantly higher in the flowers of Hokkai T10 cultivar. According to these result, between gene expression of genes related and flavonoids biosynthesis have correlation in the flowers.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Characterization of Genes Involved in Rosmarinic Acid Biosynthesis from <i>Prunella vulgaris</i>

Kim

Shin

Tuấn

et al. 2014

Biological & Pharmaceutical Bulletin

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Prunella vulgaris L., commonly known as "self-heal" or "heal-all," is a perennial herb with a long history of medicinal use. Phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), and 4-coumarate:coenzyme-A (CoA) ligase (4CL) are important enzymes in the phenylpropanoid pathway and in the accumulation of rosmarinic acid (RA), which is a major secondary metabolite in P. vulgaris. In this study, we isolated cDNAs encoding PvPAL, PvC4H, and Pv4CL from P. vulgaris using rapid amplification of cDNA ends polymerase chain reaction (PCR). The amino acid sequence alignments of PvPAL, PvC4H, and Pv4CL showed high sequence identity to those of other plants. Quantitative real-time PCR analysis was used to determine the transcript levels of genes involved in RA biosynthesis in the flowers, leaves, stems, and roots of P. vulgaris. The transcript levels of PvPAL, PvC4H, and Pv4CL1 were the highest in flowers, whereas Pv4CL2 was the highest in roots. High-performance liquid chromatography analysis also showed the highest RA content in the flowers (3.71 mg/g dry weight). We suggest that the expression of the PvPAL, PvC4H, and Pv4CL1 genes is correlated with the accumulation of RA. Our results revealed that P. vulgaris flowers are appropriate for medicinal usage, and our findings provide support for increasing RA production in this plant.

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“…Flavonol Synthase, as a key enzyme involved in the flavonol biosynthetic pathway, has been studied in many plants (Li et al, 2013;Toh et al, 2013;Takahashi et al, 2007;Falcone Ferreyra et al, 2010;Xu et al, 2012;Mahajan et al, 2011). In plants, flavonol synthase could catalyse flavonoids synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…Flavonol synthase also could play an important role in the physiological activities of plants. In tartary buckwheat, different FtFLS isoforms of buckwheat have different functions in the response to environmental stress; transcription of FtFLS1 was inhibited by the exogenous application of ABA, SA and NaCl, while FtFLS2 was not affected by ABA but up-regulated by SA and NaCl (Li et al, 2013). In A. thaliana, 6 FLS isoforms were identified from A. thaliana that exhibited tissue or cell type-specific promoter activities.…”

Section: Discussionmentioning

confidence: 99%

“…Flavonol synthase (FLS), a member of the 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, plays a major role in the flavonols biosynthesis. So far, many FLS cDNAs have been isolated from different plant species, such as Tartary buckwheat (Li et al, 2013), Camellia sinensis (Lin et al, 2007), Acacia confusa (Toh et al, 2013), Citrus unshiu (Wellmann et al, 2002), Harosoy (Takahashi et al, 2007), Zea mays (Falcone Ferreyra et al, 2010) and G. biloba (Xu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Cloning and Sequence Analysis of a Flavonol Synthase (FLS2) Gene from Ginkgo biloba

Zeng¹,

Li²,

Hua³

et al. 2017

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oFlavonol Synthase, belongs to 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, was involved in flavonol biosynthesis in plants. In this study, we cloned a cDNA encoding flavonol synthase from Ginkgo biloba (designated as GbFLS2).The cDNA of GbFLS2 gene is 1251 bp and contains a 1017 bp open reading frame encoding 338 amino acids. The deduced protein of GbFLS2 displays extensive homology to FLS proteins from other plants such as Pinus radiata, Dioscorea alata, Camellia sinensis and Epimedium sagittatum. Phylogenetic analysis indicated that the GbFLS2 has a closer relationship with FLS from gymnosperm plants than from other plant species. The results suggest that GbFLS2 is a member of the 2-ODD superfamily, and it is from the same ancestor as FLS proteins of other gymnosperm plants.

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Differential stress-response expression of two flavonol synthase genes and accumulation of flavonols in tartary buckwheat

Cited by 61 publications

References 53 publications

Changes in seed growth, levels and distribution of flavonoids during tartary buckwheat seed development

Changes in seed growth, levels and distribution of flavonoids during tartary buckwheat seed development

Molecular Cloning and Characterization of Genes Involved in Rosmarinic Acid Biosynthesis from <i>Prunella vulgaris</i>

Cloning and Sequence Analysis of a Flavonol Synthase (FLS2) Gene from Ginkgo biloba

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