Cloning and Sequence Analysis of a Flavonol Synthase (FLS2) Gene from Ginkgo biloba

Abstract

A b s t r a c t A r t i c l e I n f oFlavonol Synthase, belongs to 2-oxoglutarate-dependent dioxygenase (2-ODD) superfamily, was involved in flavonol biosynthesis in plants. In this study, we cloned a cDNA encoding flavonol synthase from Ginkgo biloba (designated as GbFLS2).The cDNA of GbFLS2 gene is 1251 bp and contains a 1017 bp open reading frame encoding 338 amino acids. The deduced protein of GbFLS2 displays extensive homology to FLS proteins from other plants such as Pinus radiata, Dioscorea alata, Camellia sinensis and Epimedium sagittatum. Phylogenetic analysis indicated that the GbFLS2 has a closer relationship with FLS from gymnosperm plants than from other plant species. The results suggest that GbFLS2 is a member of the 2-ODD superfamily, and it is from the same ancestor as FLS proteins of other gymnosperm plants.

The spatio‐temporal biosynthesis of floral flavonols is controlled by differential phylogenetic MYB regulators in Freesia hybrida

“…Gene expression analysis showed that the functional FLSs were mainly expressed in young floral buds, which did not correspond well with the disproportionately high total flavonol content in the flowers and tissues in later stages of development in some plant species (Davies et al ., 1993; Holton et al ., 1993; Nielsen et al ., 2002; Liu et al ., 2019). Comparatively, more FLS genes have been isolated in other plants (Downey et al ., 2003; Owens et al ., 2008a; Ferreyra et al ., 2010; Ferreyra et al ., 2012; Xu et al ., 2012; Li et al ., 2013; Akita et al ., 2017; Zeng et al ., 2017; Matsui et al ., 2018; Sun et al ., 2019). However, none of these FLS genes have been reported to be highly expressed in the flowers and tissues in later stages of development (e.g.…”