Differential Staining of Bacteria: Gram Stain

Moyes, Rita B.; Reynolds, Jackie; Breakwell, Donald P.

doi:10.1002/9780471729259.mca03cs15

Cited by 112 publications

(99 citation statements)

References 2 publications

(1 reference statement)

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“…The cell morphology of the isolates was observed via a microscope (Nikon, Eclipse E200) after Gram‐staining and endospore‐staining were performed using standard staining protocols (Moyes et al . ).…”

Section: Methodsmentioning

confidence: 97%

Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification

Omoregie

Ong

Nissom

2018

Lett Appl Microbiol

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Biocalcification through the use of ureolytic bacteria and biochemical activities has evolved in recent decades into a fervent resourceful effective technology suitable for soil stabilization, crack repair and bioremediation. Extensive studies have been carried out on numerous ureolytic bacterial species isolated from soils and sewage samples. However, very limited attention has been given to limestone caves with natural calcite formations as a possible source for isolation of ureolytic bacteria. In this study, bacterial isolates were recovered from limestone cave samples to determine their suitability for biocalcification. Twenty‐seven morphologically distinct bacterial isolates were identified by partial 16S rRNA gene sequencing and their various genetic diversity was characterized according to their phylogenetic affiliations. Based on the molecular identification, Sporosarcina was the most abundant genus among all the ureolytic isolates, while the rest belonged to Pseudogracilibacillus and Bacillus genera. Analytical analysis on urease measurement showed that urease activities for the isolates ranged from 1·130 to 21·513 mol urea hydrolysed per minute, with isolate NB33 achieving the highest value and TSB4 achieving the lowest value. The estimated CaCO3 precipitates for the isolates ranged from 4·04 to 17·26 mg ml−1, with isolate NB30 achieving the highest value and TSB20 achieving the lowest value. The findings in this study demonstrated that the ureolytic bacteria from limestone caves are promising bio‐calcifying agents. Significance and Impact of the Study Ureolytic bacteria continues to play an important role as microbial tools used in geotechnical engineering for soil biocalcification. Microbial strains with the ability to produce urease enzyme and induce calcium carbonate mineral are often isolated from soil, water and sludge samples. However, screening for these essential microbes from extreme regions such as caves are rarely investigated. In this study, native bacteria which were isolated from limestone cave samples are identified and characterized. The findings suggested that these ureolytic bacterial isolates have the potential to serve as suitable alternative microbial agents for soil strengthening and stabilization.

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Section: Methodsmentioning

confidence: 97%

Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification

Omoregie

Ong

Nissom

2018

Lett Appl Microbiol

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“…Bacterial suspension of interest (see Basic Protocols 1 and 2) NFb, LGI, or LGI-P semi-solid medium (see recipe) Agar plates containing NFb, LGI, or LGI-P solid medium (see recipe) King B medium (see recipe) Glycerol Moyes, Reynolds, & Breakwell, 2009) NOTE: All materials should be sterile, and the protocol should be performed in a laminar flow hood.…”

Section: Methodsmentioning

confidence: 99%

Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Ambrosini

Passaglia

2017

CP Plant Biology

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Plant roots are associated with numerous and diverse types of beneficial and pathogenic microorganisms. Plant growth–promoting (rhizo)bacteria (PGPB or PGPR) are isolated from plants crops worldwide, and many of them are used as agricultural inoculants. Agricultural biofertilization and biocontrol of pathogens are eco‐friendly alternatives to chemical usage and have less energy, environmental, and economic costs. PGPB isolation and evaluation are essentials steps for determining bacteria that are able to improve plant development and productivity. In this unit, we present protocols to isolate bacteria from soil and plant roots (“putative” diazotrophic and endospore‐forming bacteria), as well to evaluate some of their beneficial characteristics for the promotion of plant growth (e.g., nitrogen fixation, production of indolic compounds and siderophores, phosphate solubilization, and 1‐aminocyclopropane‐1‐carboxylate deaminase activity). © 2017 by John Wiley & Sons, Inc.

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“…Gram-positive bacteria have a thick cell wall that absorbes a dye as discovered by Gram in 1884, while Gram-negative bacteria do not. This is due to the outer membrane that inhibits the inclusion of the dye [2,3]. LPS is essential for growth of Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

Twelkmeyer

Burström

et al. 2011

Carbohydrate Research

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Differential Staining of Bacteria: Gram Stain

Cited by 112 publications

References 2 publications

Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification

Assessing ureolytic bacteria with calcifying abilities isolated from limestone caves for biocalcification

Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Expression of a new disialyllacto structure in the lipopolysaccharide of non-typeable Haemophilus influenzae

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