Differential scanning calorimetry of porcine adipose tissues

Living planarian flatworms were probed using quasielastic neutron scattering to measure, on the pico-to-nanosecond time scale and nanometer length scale, microscopic diffusion of water and cell constituents in the planarians. Measurable microscopic diffusivities were surprisingly well defined in such a complex system as living animals. The overall variation in the microscopic diffusivity of cell constituents was found to be far lower than the variation in the microscopic diffusivity of water in planarians in a temperature range of 284.5 to 304.1 K.

Section: Resultsmentioning

confidence: 99%

Microscopic diffusion processes measured in living planarians

Mamontov

2018

“…Differential Scanning Calorimetry (DSC) thermogram provides a unique and sensitive signature for physicochemical transformations in bio-molecules and has been proved to be an efficient approach for demonstrating the structural changes in biological tissues 30 , 31 . To characterize the effect of storage duration on the thermal behavior of brain tissue, an aluminum pan with sealed samples of white and grey matter was cooled to 1 °C and maintained there for 0 h, 1 h and 4 h. Then the temperature was increased linearly to 60 °C.…”

Section: Resultsmentioning

confidence: 99%

Effect of in vitro storage duration on measured mechanical properties of brain tissue

Zhang

Liu

Xiong

et al. 2018

Accurate characterization of the mechanical properties of brain tissue is essential for understanding the mechanisms of traumatic brain injuries and developing protective gears or facilities. However, how storage conditions might affect the mechanical properties of brain tissue remains unclear. The objective of this study is to investigate the effect of in vitro storage duration on the mechanical performance of brain tissue since measurements are usually carried out in vitro. Differential Scanning Calorimetry (DSC) measurements and uniaxial compression mechanical experiments are carried out. The results indicate that, for brain tissue stored at 1 °C without any liquid medium, the bio-molecular interactions and the mechanical strength of both white and grey matter deteriorate with prolonged storage duration. Transmission Electron Microscopy (TEM) results reveal the degeneration of myelin sheaths and the vacuolization of cristae with prolonged storage duration, suggesting that the in vitro storage duration should be carefully controlled. The findings from this study might facilitate the development of guidelines and standards for the in vitro storage of brain tissue.

“…A less significant increase is observed by further elevation to 37 °C/40 °C. This behaviour is likely to be related to the melting of numerous lipids at various temperature points and associate change of their scattering properties 31 , 32 . Porcine adipose tissue exhibits a heat induced change around 26 °C and 35 °C 32 .…”

Section: Resultsmentioning

confidence: 99%

Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues

Ghiţă

Matousek

Stone

2018

Optical properties of biological tissues can be influenced by their temperature, thus affecting light transport inside the sample. This could potentially be exploited to deliver more photons inside large biological samples, when compared with experiments at room temperature, overcoming some of difficulties due to highly scattering nature of the tissue. Here we report a change in light transmitted inside biological tissue with temperature elevation from 20 to 40 °C, indicating a considerable enhancement of photons collected by the detector in transmission geometry. The measurement of Raman signals in porcine tissue samples, as large as 40 mm in thickness, indicates a considerable increase in signal ranging from 1.3 to 2 fold, subject to biological variability. The enhancements observed are ascribed to phase transitions of lipids in biological samples. This indicates that: 1) experiments performed on tissue at room temperature can lead to an underestimation of signals that would be obtained at depth in the body in vivo and 2) that experiments at room temperature could be modified to increase detection limits by elevating the temperature of the material of interest.