Effect of in vitro storage duration on measured mechanical properties of brain tissue

Zhang, Wei; Liu, Li Fu; Xiong, Yue; Liu, Yi Fan; Yu, Sheng Bo; Wu, Chengwei; Guo, Weihong

doi:10.1038/s41598-018-19687-2

Cited by 20 publications

(12 citation statements)

References 59 publications

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“…We conclude that if the experimental conditions are carefully chosen and the tissue is kept hydrated at all times, the degeneration process of post mortem brains is rather negligible. If brain tissue is stored without any liquid medium, however, the bio-molecular interactions and the mechanical strength of brain tissue deteriorate with prolonged storage duration, for instance due to the degeneration of myelin sheaths and the vacuolization of cristae [183].…”

Section: Fig 22mentioning

confidence: 99%

Fifty Shades of Brain: A Review on the Mechanical Testing and Modeling of Brain Tissue

Budday

Ovaert

Holzapfel

et al. 2019

Arch Computat Methods Eng

290

288

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Brain tissue is not only one of the most important but also the most complex and compliant tissue in the human body. While long underestimated, increasing evidence confirms that mechanics plays a critical role in modulating brain function and dysfunction. Computational simulations-based on the field equations of nonlinear continuum mechanics-can provide important insights into the underlying mechanisms of brain injury and disease that go beyond the possibilities of traditional diagnostic tools. Realistic numerical predictions, however, require mechanical models that are capable of capturing the complex and unique characteristics of this ultrasoft, heterogeneous, and active tissue. In recent years, contradictory experimental results have caused confusion and hindered rapid progress. In this review, we carefully assess the challenges associated with brain tissue testing and modeling, and work out the most important characteristics of brain tissue behavior on different length and time scales. Depending on the application of interest, we propose appropriate mechanical modeling approaches that are as complex as necessary but as simple as possible. This comprehensive review will, on the one hand, stimulate the design of new experiments and, on the other hand, guide the selection of appropriate constitutive models for specific applications. Mechanical models that capture the complex behavior of nervous tissues and are accurately calibrated with reliable and comprehensive experimental data are key to performing reliable predictive simulations. Ultimately, mathematical modeling and computational simulations of the brain are useful for both biomedical and clinical communities, and cover a wide range of applications ranging from predicting disease progression and estimating injury risk to planning surgical procedures.

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Section: Fig 22mentioning

confidence: 99%

Fifty Shades of Brain: A Review on the Mechanical Testing and Modeling of Brain Tissue

Budday

Ovaert

Holzapfel

et al. 2019

Arch Computat Methods Eng

290

288

View full text Add to dashboard Cite

show abstract

“…The mechanical properties of biological tissues are essential for their physiological functions[1] and their regulation plays an important role in driving various physiological and pathological consequences[2]. Such regulation is based on mechanotransductional processes which initiate distinct signalling pathways[3].…”

Section: Introductionmentioning

confidence: 99%

Deciphering the mechanoresponsive role of β-catenin in Keratoconus epithelium

Amit

Padmanabhan

Janakiraman

2019

Preprint

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26 Keratoconus (KC) a disease with established biomechanical instability of the corneal stroma, is an 27 ideal platform to identify key proteins involved in mechanosensing. This study aims to investigate 28 the possible role of β-catenin as mechanotransducer in KC epithelium. KC patients were graded 29 as mild, moderate or severe using Amsler Krumeich classification. Immunoblotting and tissue 30 immunofluorescence studies were performed on KC epithelium to analyze the expression and 31 localization of β-catenin, E-cadherin, ZO1, α-catenin, Cyclin D1, α -actinin, RhoA, Rac123. Co-32 immunoprecipitation (Co-IP) of β-catenin followed by mass spectrometry of mild KC epithelium 33 was performed to identify its interacting partners. This was further validated by using epithelial 34 tissues grown on scaffolds of different stiffness. We observed down regulation of E-cadherin, α-35 catenin, ZO1 and upregulation of Cyclin D1, α -actinin and RhoA in KC corneal epithelium .β-36 catenin Co-IP from mild KC epithelium identified new interacting partners such as StAR-related 37 lipid transfer protein3 ,Dynamin-1-like protein, Cardiotrophin-1,Musculin, Basal cell adhesion 38 molecule and Protocadherin Fat 1.β-catenin localization was altered in KC which was validated in 39 vitro, using control corneal epithelium grown on different substrate stiffness. β-catenin localization 40 is dependent upon the elastic modulus of the substrate and acts as mechanotransducer by altering 41 its interaction and regulating the barrier function in corneal epithelium. 42 Keywords-β-catenin, Elastic modulus, Keratoconus, Mechanotransduction 43 Introduction 44 The mechanical properties of biological tissues are essential for their physiological functions[1]45 and their regulation plays an important role in driving various physiological and pathological 3 46 consequences [2]. Such regulation is based on mechanotransductional processes which initiate 47 distinct signalling pathways [3].The cell specific and molecular basis of mechanosensing and the 48 sequence of biochemical reactions that ensue, has been the focus of recent research [4]. Several 49 molecules have been proposed as possible mechanosensors such as integrins, ion channels, G-50 protein-coupled receptors, and cell-cell junctional proteins [5]. 51 Keratoconus (KC) is a bilateral progressive visually debilitating clinical condition characterized 52 by abnormal thinning and protrusion of the cornea in response to biomechanical instability of 53 the corneal stroma. The etiology of the disease is believed to be multifactorial with genetic and 54 environmental factors being implicated [6]. A decrease in the elastic modulus of the stromal 55 collagen is believed to initiate a cascade of events that results in morphological and biochemical 56 changes that affects the shape and the optical function of the cornea. Morphological changes in 57 the corneal epithelium have been well documented and are considered to be one of the earliest 58 detectable changes observed in KC [7]. Based on the hypothesis t...

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“…It is a fast and environmentally friendly technique, as it does not require the use of chemical reagents. In medical sciences, there are reports showing that DSC analyses can complement histological or biochemical analyses, both in experimental works [18,19] and, potentially, in diagnostics where it has been acknowledged as a novel tool for diagnosing and monitoring several diseases [20][21][22][23][24]. In recent years, DSC is also becoming a more widely used method for analyzing animal tissues and food of animal origin [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Effect of Soybean Meal Substitution by Raw Chickpea Seeds on Thermal Properties and Fatty Acid Composition of Subcutaneous Fat Tissue of Broiler Chickens

Paszkiewicz

Muszyński

Kwiecień

et al. 2020

Animals

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Simple Summary: A soybean meal, commonly used as a primary source of protein in animal diets, is often obtained from processing genetically modified soybean varieties. The tendency to reduce the use of feeds containing transgenic constituents increases the need for alternative sources of dietary protein. In this study, we fed broiler chickens diets containing either soybean meal or raw chickpea seeds. We examined the effect of such a substitution on broilers' subcutaneous fat tissue, which has numerous important physiological functions in chickens.Abstract: In this study, the effect of soybean meal substitution by raw chickpea seeds on the thermal properties and fatty acid profile of subcutaneous fat tissue of broiler chickens was examined. The experiment, performed on Ross 308 chickens, lasted for 42 days. Tight subcutaneous fat tissue was analyzed using differential scanning calorimetry (DSC) measurements while the fatty acid composition of subcutaneous adipose tissue was determined chromatographically. There was no effect of soybean meal substitution on fat crystallization temperature or crystallization enthalpy. However, the total calorimetric enthalpy of the melting of low-melting monounsaturated and saturated triacylglycerols differed between groups. Fatty acid proportions in the subcutaneous fat tissue of broiler chickens were also altered. Among others, chickpea seed inclusion decreased the content of main saturated acid (palmitic acid) and increased the content of main monounsaturated (oleic) and tri-unsaturated (linolenic) acids. The results show that the soybean meal substitution by raw chickpea seeds in the feed can affect the structural properties of adipose tissue in broiler chickens, including the thermal transformation of unsaturated fatty acids. Due to the numerous physiological functions of subcutaneous fat tissue, understanding these mechanisms can promote the use of alternative protein both in poultry and human nutrition.

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Effect of in vitro storage duration on measured mechanical properties of brain tissue

Cited by 20 publications

References 59 publications

Fifty Shades of Brain: A Review on the Mechanical Testing and Modeling of Brain Tissue

Fifty Shades of Brain: A Review on the Mechanical Testing and Modeling of Brain Tissue

Deciphering the mechanoresponsive role of β-catenin in Keratoconus epithelium

Effect of Soybean Meal Substitution by Raw Chickpea Seeds on Thermal Properties and Fatty Acid Composition of Subcutaneous Fat Tissue of Broiler Chickens

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