Differential scanning calorimetry (DSC) analysis of human plasma in melanoma patients with or without regional lymph node metastases

Fekecs, Tamás; Zapf, István; Ferencz, A.; Lőrinczy, D.

doi:10.1007/s10973-011-1800-6

Cited by 57 publications

(45 citation statements)

References 16 publications

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“…For healthy individuals the maximum of serum or plasma transition at about 62°C and a shoulder in the higher temperature range, at about 70°C, have appeared [3][4][5][6][7]. Whereas for averaged serum transition observed for men participating in our study, three local maxima are visible (Fig.…”

Section: Resultsmentioning

confidence: 50%

“…1, the mean DSC curve (the excess specific heat capacity as a function of temperature) of serum coming from sportsmen before the exercise test is shown. It may be compared with the mean plasma or serum DSC curves reported previously for healthy individuals [3][4][5][6][7]. Generally, plasma and serum curves should be very similar, although the kind of solvent used for serum dissolution may be an important origin of differences.…”

Section: Resultsmentioning

confidence: 99%

“…For global analysis of plasma or serum the differential scanning calorimetry (DSC) can also be applied. This technique was shown recently as valuable tool for disease diagnosis [3][4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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DSC serum profiles of sportsmen

Michnik

Drzazga

Poprzęcki

et al. 2013

J Therm Anal Calorim

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The blood serum of young athletes has been investigated by differential scanning calorimetry (DSC) method. The component peaks of complex endothermic transition corresponding to serum denaturation may be correlated with the main protein fractions of serum: albumin, alpha-1, alpha-2, beta-and gamma-globulins. The averaged shape of thermal transition observed for serum of sportsmen participating in our pilot study differs from that reported previously for healthy subjects. One of the reason for the difference may be the lower than normal value of albumins to globulins ratio found for individuals in our group of athletes. Modifications of serum DSC transition due to the exercise test and 1 and 2 h rest have been observed. Statistically based conclusions are not currently possible because of the low number of participants in the study. The averaged responses to the effort and to the rest have been shown clearly different for athletes classified as the best and typical. The first moment of the transition with respect to the temperature axis has been found significantly lower for the group of athletes considered as the best.

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Section: Resultsmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 99%

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DSC serum profiles of sportsmen

Michnik

Drzazga

Poprzęcki

et al. 2013

J Therm Anal Calorim

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“…The thermal profiles of heat capacity changes for aqueous solutions of human blood serum from athletes have been described and discussed in details earlier [37]. Comparing DSC curves of athletes serum diluted with distilled water (pH 6.5-7.0) with curves reported for serum/plasma of healthy persons where the final pH of solutions was in the range 7.2-7.5 [25][26][27][29][30][31][32][33][34][35][36], distinct differences are visible. The main origin of these differences is probably the thermal denaturation profile of fatty acid-free fraction of albumin.…”

Section: Resultsmentioning

confidence: 99%

“…Differential scanning calorimetry (DSC) has recently been found useful in the support of various medical diagnoses [25][26][27][28][29][30][31][32][33][34][35][36] by analysis of plasma or serum heat capacity changes in the temperature range of protein components thermal denaturation. DSC profiles of biofluids are able to reflect their modified composition, changes in thermal stability of major proteome components resulting from covalent modifications or binding interactions involving disease biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Blood serum DSC analysis of well-trained men response to CrossFit training and green tea extract supplementation

Michnik

Sadowska-Krępa

Domaszewski

et al. 2017

J Therm Anal Calorim

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Differential scanning calorimetry (DSC) has been used for the detection of post-exercise changes in blood serum resulting from participation in the CrossFit (CF) training combined with green tea extract (GTE) supplementation. Blood samples from 20 well-trained men were collected at rest, immediately post-exercise and after 1 h of recovery in two trials: first before and second after CF training combined with GTE or placebo administration in the supplemented (S) and control (C) groups, respectively. Selected muscle damage biomarkers have been compared in different phases of the experiment. A significant increase in blood lactate content has been observed post-exercise in both trials in both participants' groups. The opposite trends have been noted for the C and S groups in creatine kinase (CK) activity changes recorded during the first to the second trial: an increase in CK for the control and a decrease for the supplemented group in all phases of the experiment: pre-exercise, post-exercise and after recovery. In the second trial, all CK values for the S group have been found significantly lower than the corresponding values recorded in the C group. These results suggest a mitigate effect of GTE supplementation on post-training muscle damage. DSC results did not reveal clear effects of training nor GTE supplementation on serum denaturation transition. However, interesting dependences of thermodynamic parameters describing this transition have been observed in different phases of the experiment. Statistically significant negative correlations have been found between post-training VO 2max and post-exercise thermodynamic parameters associated with haptoglobin contribution to serum denaturation transition.

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Micro‐ and Nanoscale Calorimetry for Biomedical Applications

Natesan

Bischof

2018

Theory and Applications of Heat Transfer in Humans

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Differential scanning calorimetry (DSC) analysis of human plasma in melanoma patients with or without regional lymph node metastases

Cited by 57 publications

References 16 publications

DSC serum profiles of sportsmen

DSC serum profiles of sportsmen

Blood serum DSC analysis of well-trained men response to CrossFit training and green tea extract supplementation

Micro‐ and Nanoscale Calorimetry for Biomedical Applications

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