Blood serum DSC analysis of well-trained men response to CrossFit training and green tea extract supplementation

Abstract

Differential scanning calorimetry (DSC) has been used for the detection of post-exercise changes in blood serum resulting from participation in the CrossFit (CF) training combined with green tea extract (GTE) supplementation. Blood samples from 20 well-trained men were collected at rest, immediately post-exercise and after 1 h of recovery in two trials: first before and second after CF training combined with GTE or placebo administration in the supplemented (S) and control (C) groups, respectively. Selected muscle damage biomarkers have been compared in different phases of the experiment. A significant increase in blood lactate content has been observed post-exercise in both trials in both participants' groups. The opposite trends have been noted for the C and S groups in creatine kinase (CK) activity changes recorded during the first to the second trial: an increase in CK for the control and a decrease for the supplemented group in all phases of the experiment: pre-exercise, post-exercise and after recovery. In the second trial, all CK values for the S group have been found significantly lower than the corresponding values recorded in the C group. These results suggest a mitigate effect of GTE supplementation on post-training muscle damage. DSC results did not reveal clear effects of training nor GTE supplementation on serum denaturation transition. However, interesting dependences of thermodynamic parameters describing this transition have been observed in different phases of the experiment. Statistically significant negative correlations have been found between post-training VO 2max and post-exercise thermodynamic parameters associated with haptoglobin contribution to serum denaturation transition.

Diversity in athlete’s response to strength effort in normobaric hypoxia

“…The observed complex endothermic transition represents the weighted sum of heat capacity changes connected with the thermal denaturation of the individual proteins within serum thus serum DSC curves give information about the thermodynamic stability of the most abundant serum proteins. Similar curves were presented and discussed by us earlier for aqueous solutions of serum derived from male amateur cyclists [32] and athletes who performed CrossFit [33]. The intensities of three visible local maxima (T 1 , Cp 1 ), (T 2 , Cp 2 ), (T 3 , Cp 3 ) have been found correlated with the level of: (1) albumin fatless fraction and a-2 globulins, (2) a-1and a-2-globulins, (3) b-and c-globulins, respectively.…”

“…This method enables to distinguish any slight differences between thermodynamic parameters of cancer and non-cancer diseases and norm [23][24][25][26][27][28][29][30][31]. Our earlier DSC studies [32,33] of athlete's serum indicate that this technique may be also applied in sports medicine to support sports training.…”

Whole-body cryostimulation impact on blood serum thermal denaturation profiles of cross-country skiers

“…This transition is irreversible. Usually, two or three main peaks can be distinguished in the DSC profile of healthy human blood serum, depending on the solvent used [14][15][16][17][18][19][20][21][22][23][24][25][26].…”

“…For several years, many attempts have been made to apply the differential scanning calorimetry (DSC) method in various areas of medical diagnostics and sports medicine. DSC profiles of biological fluids can be a source of valuable information about the health of patients as well as athletes [15][16][17][18][19][20][21][22][23][24][25][26][27]. The DSC method was used in patients with multiple disease entities [15][16][17][18][19][20][21][22][23][24].…”

“…The DSC method was used in patients with multiple disease entities [15][16][17][18][19][20][21][22][23][24]. The branch of research among athletes is just developing [25][26][27]. Further research is needed on various groups of athletes to effectively support sports training and help in the case of problems related to the health of athletes.…”