Differential scanning calorimetric measurement of cartilage destruction caused by Gram-negative septic arthritis

Sillinger, T.; Lőrinczy, D.; Kocsis, Béla; Kereskay, L.; Nöt, László G.; Wiegand, N.

doi:10.1007/s10973-013-3351-5

Cited by 12 publications

(7 citation statements)

References 23 publications

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“…Taking into account previous studies in case of suspected diagnosis of septic or periprosthetic infection, it may be advantageous-due to the shorter duration-a DSC [20,23] or the isoperibolic calorimeter compared to conventional microbiological cultures for the purpose of rapid diagnosis and choice of targeted antibiotic therapy [26,24,[43][44][45][46][47]. Using DSC, we found that changes of thermal characteristics of human synovial fluid samples collected of patients with different grades of osteoarthritis have shown correlation with severity of arthritis [23].…”

Section: Discussionmentioning

confidence: 93%

“…Depending on the clinical picture and the primary diagnostic results, arthroscopic joint drana, lavage, or extended joint exploration, synovectomy, necrectomy, will be considered as part of the emergency procedure. In many cases, delayed surgical intervention [20] involves extensive tissue death (synovia and dying of stabilizing ligament, and softening, detaching, and dying of cartilage surface). Typical clinical signs of infection, radiological changes, elevation of inflammatory markers, and detection of septic bone marrow diseases are already possible at an early stage.…”

Section: Introductionmentioning

confidence: 99%

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Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Dandé

Kocsis

Lőrinczy

2020

J Therm Anal Calorim

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The analysis of synovial fluid is an important method in diagnosing and handling septic arthritis. To achieve a quick diagnosis could be a great advantage in the therapy. The differential scanning calorimetry (DSC) proved to be a useful technique in the differential diagnosis of tumors using blood plasma or sera. The aim of this paper is to show up some characteristic thermal parameters in the diagnosis of different grades of arthritis, which are in good agreement with the severity of disease checked by conventional X-ray supported grading. To follow the effect of different bacterial strains, the synovial fluids were inoculated by three types of bacterial strains (with 103–105 CFU mL−1 concentrations) at 37 °C and stored trough 24 h. After that, they were denatured in 20–90 °C temperature range with 0.3 K min−1 scanning rate. The change in the maximum denaturation temperature (Tm) and calorimetric enthalpy (∆H) monitored the severity of sepsis and depended on the type of bacteria. The proliferation characteristics of bacteria should be strain specific. The synovial fluid samples inoculated with the most frequently occurring bacteria were monitored in isotherm mode (isoperibol calorimeter) at 37 °C up to the end of the proliferation. The isoperibolic scans clearly demonstrated specific, concentration-dependent representative curves in case of each bacterium (duration of proliferation, maximum of proliferation rates, etc.). Therefore, thermal analysis of human synovial fluid samples by DSC or isoperibolic calorimetry could be a useful tool in the staging of osteoarthritis and the diagnostics of septic arthritis.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Dandé

Kocsis

Lőrinczy

2020

J Therm Anal Calorim

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“…DSC was described as a sensitive method for evaluation of collagen structure [5,6,19,20]. The stability of peritoneal tissue can influence the activation of inflammatory and angiogenic reactions due to surgical stress.…”

Section: Discussionmentioning

confidence: 99%

Stability of peritoneal tissue evaluated by the means of DSC in obese patients undergoing laparoscopic cholecystectomy

Torres

Trębacz

Pietrzyk

et al. 2017

J Therm Anal Calorim

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Obesity is considered to be the leading metabolic disease in the world. The coincidence of postoperative complications is higher in obese individuals; thus, laparoscopic techniques due to their minimally invasive character became a standard in the surgical treatment of obesity. The proper function of peritoneal tissue is essential for the response to surgical trauma. Therefore, understanding the alterations of peritoneal tissue of obese individuals might explain the increased number of postoperative complications observed in obese patients. The study aimed to assess whether the stability of peritoneum is altered in obese patients and whether it corresponds to early postoperative complications in obese patients. Samples of parietal peritoneal tissue were collected at the beginning of the laparoscopic cholecystectomy from 80 patients. Differential scanning calorimetry was performed on samples defrosted at room temperature. Patients were divided into three groups according to the BMI value. For statistical analysis, ANOVA, Chi-square, Student's t test, and correlations with Pearson test were used. Significantly lower DH and higher T m were in patients in whom intraperitoneal adhesions were reported. Lower DH was reported in samples obtained from patients with temperature above 37°C in the first 24 h postoperatively. Enthalpy of thermal transition (DH) in peritoneum obtained from parous was significantly lower in comparison with the samples from nullipara. It can be concluded that DSC parameters correlated with parameters related to the early postoperative period. Occurrence of adhesions was reported more frequently in the group of obese and overweight individuals and correlated with lower change in enthalpy of collagen denaturation.

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“…Previously differential scanning calorimetry (DSC) was used for the investigation of normal and degenerative human meniscus cartilage, and water content has not been measured [11][12][13][14]. A significant paper from this field was the study of Than et al [15], further studies measured the difference between primary osteoarthritis and septic arthritis [16]. Most of the known changes in the extracellular matrix in OA come from animal models, because human samples are only available for experiment to a limited extent.…”

Section: Introductionmentioning

confidence: 99%

Thermogravimetric Investigation of Normal and Pathological Human Meniscus

Gálity¹,

Aigner²

2015

J Thermodyn Catal

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Differential scanning calorimetric measurement of cartilage destruction caused by Gram-negative septic arthritis

Cited by 12 publications

References 23 publications

Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Stability of peritoneal tissue evaluated by the means of DSC in obese patients undergoing laparoscopic cholecystectomy

Thermogravimetric Investigation of Normal and Pathological Human Meniscus

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