Differential Roles of the Universal Stress Proteins of
            <i>Escherichia coli</i>
            in Oxidative Stress Resistance, Adhesion, and Motility

Nachin, Laurence; Nannmark, Ulf; Nyström, Thomas

doi:10.1128/jb.187.18.6265-6272.2005

Cited by 280 publications

(274 citation statements)

References 27 publications

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“…We also detected an increased uspA transcript level prior to cell division. Nachin et al (2005) recently showed UspA is involved in oxidative stress resistance in E. coli, and therefore our results may indicate division induces expression of genes designed to protect cells from DNA-damaging agents.…”

Section: Transcript Level Increases Following Gene Replication For Somentioning

confidence: 99%

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

Echtenkamp

Wilson

Shuler

2008

Biotech & Bioengineering

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We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell division cycle. A two-step ANOVA technique was used to analyze data from custom oligonucleotide microarrays containing genes involved in important cellular processes including central metabolism, macromolecular synthesis, and transport and secretion. Consistent with results previously found in Caulobacter, approximately 17% of the transcript levels were cell cycle dependent. Cell cycle regulation can be divided into two classes: genes displaying increased transcript concentrations following gene replication and genes displaying an increased transcript concentration prior to replication initiation. Transcripts levels for hns, uspA, and zwf were affected by the cell division cycle, but did not fit well into either class. These results indicate that transcription of a significant fraction of the genome is affected by replication cycle progression. The results also show that both physical gene position and the physiological function of a gene affect when it is transcribed. In addition to the simple association with replication fork progression, other phenomena must be occurring to account for some of our observations. In conclusion, gene position, with regard to the C period, and gene function are important factors to incorporate into design criteria for synthetic bacterial genomes.

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Section: Transcript Level Increases Following Gene Replication For Somentioning

confidence: 99%

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

Echtenkamp

Wilson

Shuler

2008

Biotech & Bioengineering

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show abstract

“…Universal stress proteins are associated with various mechanisms of stress resistance (Nachin et al, 2005;, including long-term survival of M. tuberculosis under low-oxygen conditions . NarK2 (Rv1737c) is important for nitrate reductase activity (Sohaskey & Wayne, 2003), and may be involved in adaptation to nitric oxide stress in macrophages (Shi et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Evidence for complex interactions of stress-associated regulons in an mprAB deletion mutant of Mycobacterium tuberculosis

Pang

Byrd³

et al. 2007

Microbiology

157

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Two-component systems are important constituents of bacterial regulatory networks. Results of this investigation into the role of the MprAB two-component system of Mycobacterium tuberculosis indicate that it is associated with the regulation of several stress-responsive regulons. Using a deletion mutant lacking portions of the response regulator, MprA, and the histidine kinase, MprB, it was demonstrated by real-time PCR, primer extension analyses and DNA microarrays that MprAB activates sigma factor genes sigE and sigB, under SDS stress and during exponential growth. SDS-inducible, MprA-dependent transcriptional start points were identified for mprA, sigE and sigB, and variations in distance between these points and MprA-binding sites suggest that MprA is involved in different mechanisms of promoter activation. Although most of the SigE regulon was downregulated in the deletion mutant, the cluster of genes Rv1129c, Rv1130 and Rv1131, which is associated with growth in monoctyes, was upregulated in the deletion mutant under SDS stress, and this upregulation was dependent upon atmospheric growth conditions. Multiple stress-associated genes of the DosR, SigD and IdeR regulons were also upregulated in the deletion mutant, during exponential growth and/or in the presence of SDS. Surprisingly, the deletion mutant had increased resistance to SDS compared to the parental strain, and enhanced growth in human peripheral blood monocytes, characteristics which may result from a loss of repression of stress-associated genes. INTRODUCTIONAs evidenced by its historical and current impact on human populations (Corbett & Raviglione, 2005;Zink et al., 2005), Mycobacterium tuberculosis is a highly successful pathogen. To withstand the challenges of aerosol transmission, growth within host macrophages, and prolonged encapsulation within lung granuloma, these organisms require the ability to respond to different types of stress. M. tuberculosis has 13 sigma factors (Cole et al., 1998), and DNA microarray analyses have shown the importance of several of these in regulating the changes in gene expression patterns associated with various stresses (Geiman et al., 2004;Manganelli et al., 2001Manganelli et al., , 2002. In addition to sigma factors, the response regulators of some two-component systems (TCSs) (Rison et al., 2005), such as DosR (Kendall et al., 2004;Park et al., 2003) and PhoP Perez et al., 2001;Walters et al., 2006), and other transcriptional regulators, such as IdeR (Dussurget et al., 1999;Manabe et al., 2005) and EmbR (Sharma et al., 2006), have been shown to modulate mycobacterial gene expression in response to particular stresses. However, in general, the mechanisms by which M. tuberculosis senses a particular stress, and activates the appropriate regulatory factor(s) while inhibiting others, are largely unknown. The GEO accession numbers for the array data associated with this paper are GSM155424-155447 (series record no. GSE6750).Four supplementary tables are available with the online version of this paper.Abbreviati...

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“…Similarly, there are two catalases in E. coli: hydroperoxidase I (HPI) and hydroperoxidase II (HPII), and they are encoded by katG and katE, respectively. The gene katG is part of the H 2 O 2 dependent oxyR regulated response system, while the gene katE is regulated by RpoS independent of H 2 O 2 induction (Mukhopadhyay and Schellhorn, 1994;Chiang and Schellhorn, 2012 (Nachin et al, 2005). However, only a few studies have applied this method to investigate PC inactivation mechanisms (Gao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

Sun

et al. 2016

Water Research

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Differential Roles of the Universal Stress Proteins of Escherichia coli in Oxidative Stress Resistance, Adhesion, and Motility

Cited by 280 publications

References 27 publications

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design

Evidence for complex interactions of stress-associated regulons in an mprAB deletion mutant of Mycobacterium tuberculosis

Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

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