Differential responses of larval zebrafish to the fungicide propamocarb: Endpoints at development, locomotor behavior and oxidative stress

Liu, Xin; Zhang, Rui; Jin, Yuanxiang

doi:10.1016/j.scitotenv.2020.139136

Cited by 25 publications

(15 citation statements)

References 59 publications

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“…Under the microscope, the heart rate of D. magna and larval zebrafish was recorded to be around 200 and 150 beats per minute after the imaging experiments. The rate is consistent with those of control groups and literature reports [57,58] , HSO 3 -(10 equiv. ), S 4 2-(10 equiv.…”

Section: J O U R N a L P R E -P R O O Fsupporting

confidence: 91%

A Ruthenium(II) complex-based probe for colorimetric and luminescent detection and imaging of hydrogen sulfide in living cells and organisms

Liu

Zhang

et al. 2021

Analytica Chimica Acta

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: J O U R N a L P R E -P R O O Fsupporting

confidence: 91%

A Ruthenium(II) complex-based probe for colorimetric and luminescent detection and imaging of hydrogen sulfide in living cells and organisms

Liu

Zhang

et al. 2021

Analytica Chimica Acta

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“…Stock solutions of each compound covering the test concentration were prepared in DW and stored at 4 • C. LIN and PM were tested at a concentration of 350 µg/L, in single and combined exposure. Toxicological concentrations were chosen in a range of levels reported to have sublethal effects on developing zebrafish [13,14]. At 2-3 hpf, embryos were examined under a dissecting microscope, and those embryos that had developed normally were selected for subsequent experiments.…”

Section: Zebrafish Embryos Exposurementioning

confidence: 99%

“…The present study aims to investigate the effects of sublethal concentrations of two largely used pesticides (linuron (LIN), an herbicide, and propamocarb (PM), a fungicide) on zebrafish early life stages, after single and combined exposure. Both LIN and PM have recently been investigated in zebrafish early life stages, identifying their ability to impair neurotransmitter biosynthesis and affect the transcription levels of several neurotoxicity-related genes [ 13 , 14 ]. Regarding LIN and PM neurotoxicity, their ability to interfere with neurotransmitter production, release and uptake (e.g., gamma-aminobutyric acid (GABA), dopamine (DOPA), acetylcholine (ACh)), synapse formation, glial cells and neuronal differentiation is well documented [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Both LIN and PM have recently been investigated in zebrafish early life stages, identifying their ability to impair neurotransmitter biosynthesis and affect the transcription levels of several neurotoxicity-related genes [ 13 , 14 ]. Regarding LIN and PM neurotoxicity, their ability to interfere with neurotransmitter production, release and uptake (e.g., gamma-aminobutyric acid (GABA), dopamine (DOPA), acetylcholine (ACh)), synapse formation, glial cells and neuronal differentiation is well documented [ 13 , 14 ]. However, there is still a lack of knowledge regarding these two pesticides’ mixture effects on zebrafish early life stages, and thus studies on possible effects of the realistic environmental mixture of LIN and PM on zebrafish synaptogenesis are necessary.…”

Section: Introductionmentioning

confidence: 99%

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An Experimental Approach to Study the Effects of Realistic Environmental Mixture of Linuron and Propamocarb on Zebrafish Synaptogenesis

Caioni

Merola

Perugini

et al. 2021

IJERPH

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The reasons behind the extensive use of pesticides include the need to destroy vector organisms and promote agricultural production in order to sustain population growth. Exposure to pesticides is principally occupational, even if their persistence in soil, surface water and food brings the risk closer to the general population, hence the demand for risk assessment, since these compounds exist not only as individual chemicals but also in form of mixtures. In light of this, zebrafish represents a suitable model for the evaluation of toxicological effects. Here, zebrafish embryos were exposed for 96 h post fertilization (hpf) to sublethal concentrations (350 µg/L) of linuron and propamocarb, used separately and then combined in a single solution. We investigated the effects on morphological traits and the expression of genes known to be implicated in synaptogenesis (neurexin1a and neuroligin3b). We observed alterations in some phenotypic parameters, such as head width and interocular distance, that showed a significant reduction (p < 0.05) for the mixture treatment. After individual exposure, the analysis of gene expression showed an imbalance at the synaptic level, which was partially recovered by the simultaneous administration of linuron and propamocarb. This preliminary study demonstrates that the combined substances were responsible for some unpredictable effects, diverging from the effect observed after single exposure. Thus, it is clear that risk assessment should be performed not only on single pesticides but also on their mixtures, the toxicological dynamics of which can be totally unpredictable.

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“…The Locomotor Behavior of Larvae. Previous studies have shown that DZP and MACB at drinking water pollution level interfere with the neurodevelopment of juvenile fish; we further explored the behavioral effect of DZP and MACB on the larval fish using the dark-light test [32,33]. The eyes of larvae could detect light signals and made a judgement [34].…”

Section: Assessment Of Impact On Nervous Developmentmentioning

confidence: 99%

Diazepam and Its Disinfection Byproduct Promote the Early Development of Nervous System in Zebrafish Embryos

Zhao

Huang

Shi

et al. 2020

Oxidative Medicine and Cellular Longevity

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The widely used diazepam, as central nervous system inhibitor, has found to be ubiquitous in surface water and drinking water. Moreover, a series of byproducts such as 2-methylamino-5-chlorobenzophenone (MACB) were generated after the chlorine disinfection process. However, little information is available about the neurobiological effects of these emerging chemicals at low doses, especially on infants and children. Here, we exposed zebrafish (Danio rerio) embryos to diazepam and MACB at 0.05, 0.5, and 5 nM, which were equivalent to environmental levels. Both diazepam and MACB increased the somite number and promoted nervous development of transgenic zebrafish [Tg (elavl3: EGFP) larvae] at 72 hours postfertilization ( hpf). Both diazepam and MACB also disrupted the homeostasis of adenosine monophosphate, valine, methionine, and fumaric acid in zebrafish embryos at 12 hpf. Additionally, the locomotor behavior activity of zebrafish was significantly enhanced after 120-hour sustained exposure to diazepam or MACB. Moreover, the mRNA expression levels of oct4, sox2, and nanog, modulating the pluripotency and self-renewal, were upregulated by diazepam and MACB in zebrafish embryo. Altogether, diazepam and MACB stimulate developmental neurogenesis and may induce neuronal excitotoxicity at quite low doses. These results indicated that the chronic exposure to psychoactive drugs may pose a potential risk to the development of the nervous system in infancy.

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Differential responses of larval zebrafish to the fungicide propamocarb: Endpoints at development, locomotor behavior and oxidative stress

Cited by 25 publications

References 59 publications

A Ruthenium(II) complex-based probe for colorimetric and luminescent detection and imaging of hydrogen sulfide in living cells and organisms

A Ruthenium(II) complex-based probe for colorimetric and luminescent detection and imaging of hydrogen sulfide in living cells and organisms

An Experimental Approach to Study the Effects of Realistic Environmental Mixture of Linuron and Propamocarb on Zebrafish Synaptogenesis

Diazepam and Its Disinfection Byproduct Promote the Early Development of Nervous System in Zebrafish Embryos

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