Differential Regulation of Gap Junction Protein (Connexin) Genes during Cardiomyocytic Differentiation of Mouse Embryonic Stem Cellsin Vitro

Oyamada, Yumiko; Komatsu, Kiyoshi; Kimura, Hisakazu; Mori, Makoto; Oyamada, Masahito

doi:10.1006/excr.1996.0377

Cited by 74 publications

(66 citation statements)

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“…Such arrhythmogenic potential could become prominent in species whose heart rates are much slower (e.g., ~80 bpm for humans). Like MSCs [40], pluripotent ESCs also express gap junction proteins [41][42][43] for electrical coupling. Furthermore, ESCs can even subsequently differentiate into electrically active lineages [7,39,44,45].…”

Section: Discussionmentioning

confidence: 99%

Electrophysiological Properties of Pluripotent Human and Mouse Embryonic Stem Cells

et al. 2005

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Pluripotent embryonic stem cells (ESCs) possess promising potential for cell-based therapies, but their electrophysiological properties have not been characterized. Here we describe the presence of ionic currents in mouse (m) and human (h) ESCs and their physiological function. In mESCs, tetraethylammonium (TEA) -sensitive depolarization-activated delayed rectifier K + currents (IK DR ) (8.6 ± 0.9 pA/pF at +40 mV; IC 50 = 1.2 ± 0.3 mM), which contained components sensitive to 4-aminopyridine (4-AP) (IC 50 = 0.5 ± 0.1 mM) and 100 nM Ca 2+ -activated K + current (IK Ca ) blocker iberiotoxin (IBTX), were detected in 52.3% of undifferentiated cells. IK DR was similarly present in hESCs (~100%) but with an approximately sixfold higher current density (47.5 ± 7.9 pA/pF at +40 mV). When assayed by bromodeoxyurindine incorporation, application of TEA, 4-AP, or IBTX significantly reduced the proliferation of mESCs and hESCs in a dose-dependent manner (p < .05). A hyperpolarization-activated inward current (I h ) (−2.2 ± 0.4 pA/pF at −120 mV) was detected in 23% of mESCs but not hESCs. Neither Na v nor Ca v currents were detected in mESCs and hESCs. Microarray and reverse transcription-polymerase chain reaction analyses identified several candidate genes for the ionic currents discovered. Collectively, our results indicate that pluripotent ESCs functionally express several specialized ion channels and further highlight similarities and differences between the two species. Practical considerations for the therapeutic use of ESCs are discussed. Stem Cells 2005;23:1526-1534

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Section: Discussionmentioning

confidence: 99%

Electrophysiological Properties of Pluripotent Human and Mouse Embryonic Stem Cells

et al. 2005

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“…Además, similar a lo reportado por Oyamada y cols., observamos una sobreexpresión de giros β la cual está relacionada con un aumento de las uniones gap durante la diferenciación de CMP [10] .…”

Section: Tratamiento Espectral Y Análisis Multivariadounclassified

“…Respecto al segundo componente principal en las primera y segunda regiones (proteínas y carbohidratos), este análisis nos posibilitó discriminar la primera fase de diferenciación (día 11), en la cual como se mencionó anteriormente, se ha reportado la expresión de nuevas proteínas relacionado con el aumento en la complejidad celular y en el contenido proteico, así como el aumento de las moléculas de azúcar por el metabolismo de los carbohidratos como el glucógeno durante la diferenciación de CMP [2,8,10] . Además, esta segunda región analizada correspondiente al glucógeno, refuerza mediante ACP lo reportado por Walsh y cols., quienes mencionan que este intervalo espectral, constituye un buen indicador de las alteraciones en la estructura secundaria del DNA y lo destacan como una región fundamental para el proceso de diferenciación [15] .…”

Section: Tratamiento Espectral Y Análisis Multivariadounclassified

Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Mata-Miranda

Guerrero-Robles

Rojas-López

et al. 2017

Rev. Mex. Ing. Biomed.

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RESUMENDos de los grandes retos en la biología de las Células Madre (CM) y la Medicina Regenerativa, son el control en la diferenciación de estas células y asegurar la pureza de las células diferenciadas, por lo que es necesario contar con técnicas rápidas, eficientes y precisas para la caracterización de CM y su diferenciación a diferentes linajes celulares. El objetivo de este trabajo fue analizar Células Madre Pluripotentes (CMP) y Células Pancreáticas Diferenciadas (CPD) mediante espectroscopía Infrarroja por Transformada de Fourier (FTIR) y Análisis de Componentes Principales (ACP). Para ello se diferenciaron CMP a CPD, caracterizando el proceso de diferenciación a los días 0, 11, 17 y 21 mediante microscopía óptica y espectroscopia vibracional. Los espectros FTIR se analizaron con el método multivariado de ACP, utilizando su segunda derivada en las regiones de proteínas, carbohidratos y ribosas. Los resultados indican que el ACP permite caracterizar y discriminar CMP y CPD en sus diferentes etapas de diferenciación en las regiones espectrales analizadas. Con lo anterior concluimos que el ACP permite caracterizar química y estructuralmente CMP y diferentes etapas de su diferenciación en una forma rápida, precisa y no invasiva.

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“…and 1682 cm -1 , that suggested that the expression of proteins typical of cardiomyocyte precursors was taking place. Indeed, it is known that these cells are rich in alpha-myosin, a protein belonging to alpha-helix fold and that they are characterized by the formation of gap junctions (Oyamada et al, 1996), whose main protein components are connexins, containing again alpha-helix structures and an important percentage of beta-turns. To support our hypothesis -confirmed by cytochemical analysis -after the "switch" of the new phenotype we also observed the emergence of IR bands due to glycogen at 1155 cm -1 , 1081 cm -1 and between 1035 and 1020 cm -1 , typical of cardiomyocytes (Pasumarthi & Field 2002).…”

Section: Embryonic Stem Cellsmentioning

confidence: 99%

Fourier Transform Infrared Microspectroscopy as a Tool for Embryonic Stem Cell Studies

Ami¹,

Mereghetti²,

Natalello³

et al. 2011

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applicatio

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Differential Regulation of Gap Junction Protein (Connexin) Genes during Cardiomyocytic Differentiation of Mouse Embryonic Stem Cellsin Vitro

Cited by 74 publications

References 0 publications

Electrophysiological Properties of Pluripotent Human and Mouse Embryonic Stem Cells

Electrophysiological Properties of Pluripotent Human and Mouse Embryonic Stem Cells

Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Fourier Transform Infrared Microspectroscopy as a Tool for Embryonic Stem Cell Studies

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