Differential regulation of duplicate alcohol dehydrogenase genes in Drosophila mojavensis

Batterham, Philip; Lovett, Janice; Starmer, William T.; Sullivan, David T.

doi:10.1016/0012-1606(83)90172-0

Cited by 49 publications

(13 citation statements)

References 23 publications

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“…This hypothesis explains the early adaptive evolution of the Adh region, the similarity of the amino acid changes, and the fact that these substitutions occurred at conserved sites. Moreover, this hypothesis also may partially explain why these CFG proteins were not observed on Adh allozyme gels, although it is difficult to exclude the effects of reduced gene expression in the CFGs (14,(31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Parallel evolution of chimeric fusion genes

Jones

Begun

2005

Proc. Natl. Acad. Sci. U.S.A.

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To understand how novel functions arise, we must identify common patterns and mechanisms shaping the evolution of new genes. Here, we take advantage of data from three Drosophila genes, jingwei, Adh-Finnegan, and Adh-Twain, to find evolutionary patterns and mechanisms governing the evolution of new genes. All three of these genes are independently derived from Adh, which enabled us to use the extensive literature on Adh in Drosophila to guide our analyses. We discovered a fundamental similarity in the temporal, spatial, and types of amino acid changes that occurred. All three genes underwent rapid adaptive amino acid evolution shortly after they were formed, followed by later quiescence and functional constraint. These genes also show striking parallels in which amino acids change in the Adh region. We showed that these early changes tend to occur at amino acid residues that seldom, if ever, evolve in Drosophila Adh. Changes at these slowly evolving sites are usually associated with loss of function or hypomorphic mutations in Drosophila melanogaster. Our data indicate that shifting away from ancestral functions may be a critical step early in the evolution of chimeric fusion genes. We suggest that the patterns we observed are both general and predictive.Drosophila ͉ neomorph

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Section: Discussionmentioning

confidence: 99%

Parallel evolution of chimeric fusion genes

Jones

Begun

2005

Proc. Natl. Acad. Sci. U.S.A.

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“…In contrast, the D. muUeriAdh locus contains two functional genes: one that is expressed in larvae and one that is expressed in adults [Adh-2) Fischer andManiatis 1985, 1986). These genes produce two distinct ADH proteins (Oakeshott et al 1982;Batterham et al 1983Batterham et al , 1984.…”

mentioning

confidence: 99%

“…The Drosophila melanogaster Adh gene is transcribed during embryogenesis, larval development, and in adults, and it is expressed in a specific set of tissues that includes the fat body, gut, and reproductive organs (Ursprung et al 1970;Savakis et al 1986;Lockett and Ashburner 1989). The pattern of Adh tissue-specific expression varies from species to species, but all species thus far examined express Adh in the larval and adult fat body (Dickinson 1980a(Dickinson ,b, 1988Batterham et al 1983;Rabinow and Dickinson 1986).…”

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confidence: 99%

A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements.

Abel¹,

Bhatt²,

Maniatis³

1992

Genes Dev.

117

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We have identified a Drosophila transcription factor that binds to fat body-specific enhancers of alcohol dehydrogenase {Adh) and yolk protein genes. DNA sequence analysis of cDNA clones encoding this protein, box B-binding £actor-2 (BBF-2), indicates that it is a member of the CREB/ATF family of transcriptional regulatory proteins. A number of observations suggest that BBF-2 is involved in fat body-specific expression: Mutations that disrupt BBF-2 binding to two different Adh fat body enhancers in vitro decrease the activity of these enhancers in transgenic flies. BBF-2 mRNA is present in all cell types examined, and the protein is present in cells that express ADH. Finally, BBF-2 is a transcriptional activator in Drosophila tissue culture cells. Remarkably, BBF-2 also binds specifically to regulatory elements required for liver-specific expression of the human Adh and rat tyrosine aminotransferase genes. Thus, BBF-2 and the DNA sequence to which it binds may be important components of a tissue-specific regulatory mechanism conserved between Drosophila and man.

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“…On a food medium rich in sucrose, Amy3 and Amy6 were only expressed in a small region of the PMG. Matsuo and Yamazaki (1986) In some aspects the amylase system of D. ananassae actually resembles the duplicated Adh systems of species of the mullen subgroup (Batterham et al, 1983a(Batterham et al, , 1983b(Batterham et al, , 1984Mills et at., 1986) and the fourloci esterase system of D. montana (Roberts & Baker, 1973;Baker, 1975Baker, , 1980 in which duplications were followed by tissue-specific and developmentally separate regulation.…”

Section: Resultsmentioning

confidence: 99%

Variation in sex-, stage- and tissue-specific expression of the amylase genes in Drosophila ananassae

Lage

Klarenberg²,

Cariou

1996

Heredity

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Expression of the amylase multigene family of Drosophila ananassae was investigated in thirdinstar larvae and adults. A developmental differentiation was found between the Amyl-2 and Amy3-4 gene clusters, the former being preferentially expressed in larvae, the latter in adults. During adult life, we observed a decrease in Amy] -2 expression in males of certain strains. We have raised some arguments for the existence of trans-active regulators, acting as repressors of Amy]-2 in adults. The putative repressors might exhibit a geographical polymorphism, with a fixed active form in Pacific regions and a polymorphic pattern in Africa, thus increasing the diversity observed in adult amylase phenotypes. A clear differentiation between the two gene clusters was also found in tissue-specific activity along the third-instar larval midgut. In the anterior midgut, only Amy]-2 is active, while both gene groups are expressed in the posterior midgut, with an additional subzonation within it.

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Differential regulation of duplicate alcohol dehydrogenase genes in Drosophila mojavensis

Cited by 49 publications

References 23 publications

Parallel evolution of chimeric fusion genes

Parallel evolution of chimeric fusion genes

A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements.

Variation in sex-, stage- and tissue-specific expression of the amylase genes in Drosophila ananassae

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