A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements.

Abel, Ted; Bhatt, Rupal S.; Maniatis, Tom

doi:10.1101/gad.6.3.466

Cited by 117 publications

(74 citation statements)

References 77 publications

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“…At present the roles of LZIP, Luman, and the g726428 protein are not known. BBF-2/dCREB-A is expressed in a several adult and embryonic tissues and may be involved in regulating the alcohol dehydrogenase gene in yolk and fat body (1). Recent evidence suggests that during embryogenesis, it functions in signaling cascades that decide the patterning of cuticular structures (3).…”

Section: Discussionmentioning

confidence: 99%

Luman, a New Member of the CREB/ATF Family, Binds to Herpes Simplex Virus VP16-Associated Host Cellular Factor

Yang

O’Hare

et al. 1997

Molecular and Cellular Biology

167

212

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The human host cell factor (HCF) is expressed in a variety of adult and fetal tissues, and its gene is conserved in animals as diverse as mammals and insects. However, its only known function is to stabilize the herpes simplex virus virion transactivator VP16 in a complex with the cellular POU domain protein Oct-1 and cis-acting regulatory elements in promoters of immediate-early viral genes. To identify a cellular function for HCF, we used the yeast two-hybrid system to identify a cellular ligand for HCF. This protein, Luman, appears to be a cyclic AMP response element (

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Section: Discussionmentioning

confidence: 99%

Luman, a New Member of the CREB/ATF Family, Binds to Herpes Simplex Virus VP16-Associated Host Cellular Factor

Yang

O’Hare

et al. 1997

Molecular and Cellular Biology

167

212

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“…Two of the proteins, C/EBP and BBF2, are activators of Adh and the third, AEF1, is a repressor (Abel et al 1992;Falb and Maniatis 1992). All three of these proteins bind to FBE in vitro, suggesting that C/EBP and BBF2 may be responsible for the tissue-specific activation by FBE (Abel et al 1992;Falb and Maniatis 1992; W.C. An and P.C.…”

Section: Activation Of Transcription In Fat Bodiesmentioning

confidence: 99%

Sex-specific transcriptional regulation by the male and female doublesex proteins of Drosophila.

Coschigano¹,

Wensink²

1993

Genes Dev.

184

148

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The somatic sexual phenotype of Drosophila is regulated by the sexual differentiation pathway. Male {DSX M) and female {DSX F) proteins encoded by doublesex {dsx), a gene at the end of this pathway, bind to three sites within a 127-bp enhancer that directs sex-and tissue-specific transcription of Yolk protein genes. We describe mutagenesis of these binding sites and the resulting effects on DSX M and DSX F binding in vitro and on gene regulation in wild-type and dsx mutant flies. The results demonstrate that DSX ~ represses and DSX F activates transcription from the two strongest binding sites. Thus, the pathway regulates sex-specific transcription through the male and female dsx proteins that act directly on the target gene, but with opposite effects.

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“…Using the transgenic mice expressing dominant negative CREB, CREB was shown to control hepatic lipid metabolism and gluconeogenesis in response to a series of hormonal cues (Herzig et al, 2001(Herzig et al, , 2003. In Drosophila cultured cells, box-B-binding factor-2 (BBF-2), a member of ATF/CREB family, binds specifically to the fat body-specific regulator element to activate transcription (Abel et al, 1992). Obesity increases total JNK activity and JNK1-deficient mice exhibit reduced adiposity and improved insulin sensitivity (Hirosumi et al, 2002).…”

Section: Knockdown Flies As a Model Suitable For Study Of The Role Ofmentioning

confidence: 99%

ATF-2 Regulates Fat Metabolism inDrosophila

Okamura

Shimizu²,

Nagao³

et al. 2007

MBoC

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ATF-2 is a member of the ATF/CREB family of transcription factors that is activated by stress-activated protein kinases such as p38. To analyze the physiological role of Drosophila ATF-2 (dATF-2), we generated dATF-2 knockdown flies using RNA interference. Reduced dATF-2 in the fat body, the fly equivalent of the mammalian liver and adipose tissue, decreased survival under starvation conditions. This was due to smaller triglyceride reserves of dATF-2 knockdown flies than control flies. Among multiple genes that control triglyceride levels, expression of the Drosophila PEPCK (dPEPCK) gene was strikingly reduced in dATF-2 knockdown flies. PEPCK is a key enzyme for both gluconeogenesis and glyceroneogenesis, which is a pathway required for triglyceride synthesis via glycerol-3-phosphate. Although the blood sugar level in dATF-2 knockdown flies was almost same as that in control flies, the activity of glyceroneogenesis was reduced in the fat bodies of dATF-2 knockdown flies. Thus, reduced glyceroneogenesis may at least partly contribute to decreased triglyceride stores in the dATF-2 knockdown flies. Furthermore we showed that dATF-2 positively regulated dPEPCK gene transcription via several CRE half-sites in the PEPCK promoter. Thus, dATF-2 is critical for regulation of fat metabolism.

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A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements.

Cited by 117 publications

References 77 publications

Luman, a New Member of the CREB/ATF Family, Binds to Herpes Simplex Virus VP16-Associated Host Cellular Factor

Luman, a New Member of the CREB/ATF Family, Binds to Herpes Simplex Virus VP16-Associated Host Cellular Factor

Sex-specific transcriptional regulation by the male and female doublesex proteins of Drosophila.

ATF-2 Regulates Fat Metabolism inDrosophila

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