Differential Quantitative Analysis of Bile Acids in Bile and in Duodenal Drainage

Cholesterol Metabolism in Man

“…Assay of these three fractions indicated (Table II) that cholesterol is excreted in bile primarily as a bile acid(s). In the four bile acid samples analyzed only about 10 per cent of the C14 was found in the nonsaponifiable fraction; negligible activity was present in the form of fatty acids. On the other hand, between 89 and 93 per cent of the biliary C14 was found in the bile acids, isolated by ethyl ether extraction and precipitation according to the Doubilet procedure.…”

“…Following acidification the bile was again shaken with petroleum ether to extract fatty acids. Then ethyl ether extraction was used to obtain the bile acid fraction which was finally purified through the Doubilet procedure (10) to yield total bile acid-C".…”

Observations on bile-acid enteroliths with an account of a recent case

“…But the formation of stones within the intestine would appear to require the precipitation of the material concerned in appreciable quantity, yet it is believed that unconjugated bile-acids are present in bile in quite small amounts. Doubilet (1936) gives some results of " typical examples " of differential bile-acid analysis; these show, in human gall-bladder bile, 0.890 per cent of unconjugated acids, out of a total of 9.751 per cent, and in human fistula bile 0.413 per cent out of a total of 1.717 per cent. Not all of the unconjugated acids is deoxycholic acid, capable of combination with the free fatty acid in bile or in the intestine to form choleic acid.…”

The Composition of Human Gallbladder Bile and Its Relationship to Cholelithiasis

“…That there is a change in the character of bile acids secreted under certain abnormal conditions is suggested by the work of Greene, Walters and Fredrickson (15), of Ravdin, Johnston, Riegel and Wright (16), and of Breusch and Johnston (17). Doubilet (13) recently has demonstrated such a qualitative alteration. Doubtless the high undetermined anion concentration, frequently observed in our series in conjunction with relatively low cholate, is the result of a similar change in the type of bile acid secreted by the liver.…”

“…Owing to the fact that the modified Pettenkofer reaction employed for the determination of bile acids is specific for the cholic acids (Reinhold and Wilson (4) ), other bile acids that occur in human bile are included in the undetermined anion.' Desoxycholic acid appears to be most important quantitatively, according to Wieland and Reverey (12) and Doubilet (13). Chenodesoxycholic acid (12) and lithocholic acid (14) have been isolated from human bile and from gallstones, although data are not available relative to their concentration.…”