Differential pulse voltammetry and chronoamperometry as analytical tools for epinephrine detection using a tyrosinase-based electrochemical biosensor

Baluta, Sylwia; Meloni, Francesca; Szyszka, A.; Zucca, Antonio; Pilo, Maria Itria; Cabaj, Joanna

doi:10.1039/d2ra04045j

Cited by 27 publications

(15 citation statements)

References 60 publications

(76 reference statements)

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“…Better LOD and a wider linear range was obtained with DPV; therefore, this technique was used for the sensitivity calculation. The biosensor thus obtained was successfully validated in a pharmaceutical product with a recovery of about 98% [ 185 ].…”

Section: Tyrosinase Immobilization Strategiesmentioning

confidence: 99%

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

Bounegru

Apetrei

2023

Nanomaterials

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The development of enzyme biosensors has successfully overcome various challenges such as enzyme instability, loss of enzyme activity or long response time. In the electroanalytical field, tyrosinase is used to develop biosensors that exploit its ability to catalyze the oxidation of numerous types of phenolic compounds with antioxidant and neurotransmitter roles. This review critically examines the main tyrosinase immobilization techniques for the development of sensitive electrochemical biosensors. Immobilization strategies are mainly classified according to the degree of reversibility/irreversibility of enzyme binding to the support material. Each tyrosinase immobilization method has advantages and limitations, and its selection depends mainly on the type of support electrode, electrode-modifying nanomaterials, cross-linking agent or surfactants used. Tyrosinase immobilization by cross-linking is characterized by very frequent use with outstanding performance of the developed biosensors. Additionally, research in recent years has focused on new immobilization strategies involving cross-linking, such as cross-linked enzyme aggregates (CLEAs) and magnetic cross-linked enzyme aggregates (mCLEAs). Therefore, it can be considered that cross-linking immobilization is the most feasible and economical approach, also providing the possibility of selecting the reagents used and the order of the immobilization steps, which favor the enhancement of biosensor performance characteristics.

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Section: Tyrosinase Immobilization Strategiesmentioning

confidence: 99%

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

Bounegru

Apetrei

2023

Nanomaterials

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“…In addition, differential pulse voltammetry (DPV) is a broadly used electrochemical technique. In this technique, the measured current difference at two points immediately before and after the potential applied to the biosensor rises is expressed as a graph of voltage [ 59 ]. Since this technique measures the current difference obtained before and after the potential rises, it is less disturbed by current measurement disturbances such as electrical double layers.…”

Section: Components Of Enzymatic Biosensorsmentioning

confidence: 99%

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Choi

Yoon

2023

Biosensors

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The detection of small molecules has attracted enormous interest in various fields, including the chemical, biological, and healthcare fields. In order to achieve such detection with high accuracy, up to now, various types of biosensors have been developed. Among those biosensors, enzymatic biosensors have shown excellent sensing performances via their highly specific enzymatic reactions with small chemical molecules. As techniques used to implement the sensing function of such enzymatic biosensors, electrochemical and fluorescence techniques have been mostly used for the detection of small molecules because of their advantages. In addition, through the incorporation of nanotechnologies, the detection property of each technique-based enzymatic nanobiosensors can be improved to measure harmful or important small molecules accurately. This review provides interdisciplinary information related to developing enzymatic nanobiosensors for small molecule detection, such as widely used enzymes, target small molecules, and electrochemical/fluorescence techniques. We expect that this review will provide a broad perspective and well-organized roadmap to develop novel electrochemical and fluorescent enzymatic nanobiosensors.

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“…Cyclic voltammetry operates based on Ohm's law (V = RI), where the current ow on the electrode is directly proportional to the analyte concentration (antigen, DNA, etc). In this technique, the current is measured between the working and counter electrodes, while the potential difference is measured between the reference and working electrodes (Baluta et al 2022). When 1mM methylene blue was added as a redox indicator in cyclic voltammetry, the current ow increased due to the binding of methylene blue to the guanine base of DNA.…”

Section: Electrochemical Characterization Of Dna Sensormentioning

confidence: 99%

Electrochemical DNA-Based Sensor for the Detection of Klebsiella pneumoniae: A Prominent Causal Agent of Urinary Tract Infections

Florien

Usha

Sharma

et al. 2023

Preprint

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Objective The aim of the study is to develop an electrochemical DNA based sensor for detection of Klebsiella pneumonia a prominent causal agent of UTI by immobilizing a 5’ NH2 labelled ssDNA probe specific to fimH gene of Klebsiella pneumonia on GQDs modified screen printed electrode. Results Present study involves the synthesis of graphene quantum dots (GQDs) based nanoparticles using hydrothermal method, and characterized using Fourier Transforming Infra-Red spectroscopy FTIR, Particle size analyzer and Fluorescence spectroscopy. The synthesized nanoparticles were dropping cast onto the screen-printed carbon electrode (SPCE) surface and used in electrochemical biosensor for detecting Klebsiella pneumonia, which is among the world’s leading pathogen causing urinary tract infections. In this study, specific NH2 labeled probe was immobilized onto GQDs fabricated electrode surface and the electrochemical response was recorded by cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) using methylene blue as a redox indicator. Electrode surface characterizations were performed using FTIR spectroscopy. This nanofabricated chip was found to be very specific, user friendly, less time consuming and affordable to everyone. The developed sensor revealed a fabulous sensitivity of 0.0002 mA/mm2/ng with a limit of detection (LOD) of 3953 ng/µL. Conclusion The GQDs modified paper electrode based genosensor for detection of Klebsiella pneumonia showed an outstanding sensitivity as well as limit od detection denoted by revealing the smallest concentration of this pathogen from patient sample.

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Differential pulse voltammetry and chronoamperometry as analytical tools for epinephrine detection using a tyrosinase-based electrochemical biosensor

Abstract: GCE/poly-4,4′-bBT/tyrosinase biosensor for epinephrine was constructed. Comparison of differential pulse voltammetry (DPV) and chronoamperometry was performed. DPV showed more reproducible results giving high selectivity, sensitivity, stability.

Cited by 27 publications

References 60 publications

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors—A Review

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Electrochemical DNA-Based Sensor for the Detection of Klebsiella pneumoniae: A Prominent Causal Agent of Urinary Tract Infections

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