Differential Pulse Voltammetric Detection of Hepatitis C Virus 1a Oligonucleotide Chain by a Label‐Free Electrochemical DNA Hybridization Biosensor Using Consensus Sequence of Hepatitis C Virus 1a Probe on the Pencil Graphite Electrode

Pournaghi-Azar, M.H.; Ahour, F.; Hejazi, Mohammad Saeid

doi:10.1002/elan.200804570

Cited by 43 publications

(18 citation statements)

References 43 publications

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“…In pioneering work by Berggren et al, non-faradaic capacitive immunosensor for the detection of Human chorionic gonadotropin hormone (HCG), and interleukin-2, a microbial infection immune factor, were reported some 15 years ago on monoclonal antibody modified Self-Assembly Modified (SAM) gold electrodes. 2,3 These assays were reported to be sensitive to targets down to pg/mL levels. In similar work non-faradaic EIS methods have been reported to detect interferon with an astonishing 8 -10 ng/mL level of sensitivity in buffer at thiolated SAM-Ab interfaces.…”

Section: Introdutionmentioning

confidence: 99%

Immittance Electroanalysis in Diagnostics

et al. 2014

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Impedance derived electroanalytical assays are inherently spectroscopic (frequency resolved) and potentially exceedingly sensitive indicators of interfacial change (such as target binding at an appropriate receptor). We introduce here the use of a portfolio of mathematically derived immittance functions and related components, capable, from the same raw data sets, of enabling increased assay sensitivity and markedly shorter assay times in comparison to traditional impedance analyses. The methodology, applied herein to faradaic (redox probe amplified) and non-faradaic assays, requires no equivalent circuit analysis or prior assumption of response. Its focus is to optimize analytical potency and to enable the user to select and apply the most frequency-optimized reporter of interfacial change and to, thereafter, run rapid (optimized) analyses at single frequencies.

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Section: Introdutionmentioning

confidence: 99%

Immittance Electroanalysis in Diagnostics

et al. 2014

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“…After hybridization, DNA is treated with an acid, and the acid-released purine bases are directly determined by SWV with HPV sequence detection limit of 2 pg·mL −1 (S/N =3). 100 The specific sequence for hepatitis C virus-1 (HCV-1) was detected by Pournaghi-Azar et al, 101 using a label-free DNA hybridization biosensor. The sensor relied on the immobilization of a 20-mer oligonucleotide (containing 2 guanines and 11 cytosines) as a probe on the pencil graphite electrode.…”

Section: Nucleic Acid Electrochemical Biosensorsmentioning

confidence: 99%

“…The hybridization event was monitored by DPV of the guanine signal. 101 Huang et al 102 described a labelfree EB with dual amplification strategy based on isothermal exponential amplification coupled with hybridization chain reaction of DNAzymes nanowires. Electrochemical signals were obtained by measuring the increase in reduction current of oxidized tetramethylbenzidine sulfate, which was generated by DNAzyme in the presence of H 2 O 2 .…”

Section: Nucleic Acid Electrochemical Biosensorsmentioning

confidence: 99%

Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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“…Various articles have been released on electrochemical nucleic acid sensor for HBV in the literature [20,21], but there are few articles for detection and quantification of HCV based on simple and reliable electrochemical methods [22,23].…”

Section: Introductionmentioning

confidence: 99%

A Nucleic Acid Biosensor for Detection of Hepatitis C Virus Genotype 1a Using Poly(l-Glutamic Acid)-Modified Electrode

Dönmez

Arslan

2015

Appl Biochem Biotechnol

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An electrochemical nucleic acid biosensor based on label-free DNA detection method was prepared for the first time by using electropolymerized poly(L-glutamic acid)-modified pencil graphite electrode (PGA/PGE) for detection of hepatitis C virus genotype 1a (HCV1a). Inosine-substituted 20-mer probes related to the HCV1a were immobilized onto PGA/PGE surface by covalent linking with the formation of amide bonds. Square wave voltammetry (SWV) was used to monitor the oxidation signal of guanine in the hybridization events, which gave an oxidation peak at +1.05 V. An increase in the oxidation signal of guanine was showed by hybridization of the probe with the complementary DNA. Noncomplementary oligonucleotides were also used to investigate the selectivity of the biosensor. The proposed nucleic acid biosensor was linear in the range of 50 nM to 1.0 μM, exhibiting a limit of detection of 40.6 nM. Finally, single-stranded synthetic PCR product analogues of HCV1a were performed in optimal condition. This PGA-modified nucleic acid sensor is cost-effective and disposable, and besides, it has superior electrocatalytic effect on the oxidation of guanine.

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Differential Pulse Voltammetric Detection of Hepatitis C Virus 1a Oligonucleotide Chain by a Label‐Free Electrochemical DNA Hybridization Biosensor Using Consensus Sequence of Hepatitis C Virus 1a Probe on the Pencil Graphite Electrode

Cited by 43 publications

References 43 publications

Immittance Electroanalysis in Diagnostics

Immittance Electroanalysis in Diagnostics

Nanoscale virus biosensors: state of the art

A Nucleic Acid Biosensor for Detection of Hepatitis C Virus Genotype 1a Using Poly(l-Glutamic Acid)-Modified Electrode

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