Differential pulse polarographic determination of l-lactate with a soluble enzyme preparation

Hasebe, Kiyoshi; Hikima, Satoshi; Yoshida, Hitoshi

doi:10.1007/bf00331423

Cited by 8 publications

(1 citation statement)

References 9 publications

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“…During the investigation, we observed that the present biosensor worked very well at room temperature rather than at high temperature, as shown in Figure 4 . It has been reported in the literature that the lactate oxidase has its optimum activity at 30 °C [ 52 ], but the response of the present biosensor was found higher at room temperature due to possibly enhanced activity of the enzyme on the surface of ZnO nanorods and might be the ZnO behaved as a promoter for lactate oxidase.…”

Section: Resultsmentioning

confidence: 74%

Electrochemical L-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase

Ibupoto

Ali

Khun

et al. 2012

Sensors

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In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of l-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 × 10−4–1 × 100 mM with acceptable sensitivity about 41.33 ± 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards l-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.

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Section: Resultsmentioning

confidence: 74%