Differential proteomic analysis of midguts from Nosema ceranae-infected honeybees reveals manipulation of key host functions

Within-host competition among the honey bees pathogens Nosema ceranae and Deformed wing virus is asymmetric and to the disadvantage of the virus

“…Although, N. ceranae has been shown to induce immune suppression in honey bees (Antúnez et al, 2009;Aufauvre et al, 2014;Chaimanee et al, 2012), recent transcriptomic and proteomic studies demonstrate that N. ceranae infection is associated with oxidative stress in the ventriculus, which may constitute the main cellular immune response of the honey bee midgut to microsporidia, and potentially responsible of the cellular damage of the gut epithelium (Dussaubat et al, 2012;Vidau et al, 2014). In the mosquito Aedes aegypti, the endosymbiotic bacteria Wolbachia has been shown to activate the host innate immune system by inducing the production of reactive oxygen species (ROS) following oxidative stress, thereby controlling dengue virus (Pan et al, 2012).…”

Microsporidia–host interactions

“…Thus, microsporidia infection may be detected through the increased demand placed on the proteasome, although there are likely to be other cues as well. A recent study using a proteomics technique also supports the hypothesis that microsporidia counteracts host degradation pathways [39]. Proteomic analysis of infected and uninfected honey bee midguts identified 14 differentially expressed proteins, one of which was a proteasome subunit that was about half as abundant upon Nosema ceranae infection.…”

Infection dynamics of Nosema ceranae in honey bee midgut and host cell apoptosis

“…Nosema is primarily transmitted via the fecal-oral route between adult bees and establishes an infection in the midgut epithelium (Fries, 2010). Over the course of infection, N. ceranae has been shown to induce multiple physiological alterations in its host, including processes involved in the regulation of programmed cell death (PCD) (Doublet et al, 2017;Higes et al, 2013a;Kurze et al, 2015;Martin-Hernandez et al, 2017) and energy metabolism (Kurze et al, 2016b;Mayack and Naug, 2009;Vidau et al, 2014). This might be not surprising as microsporidia rely on their host metabolism for supply of energy resources (Keeling, 2009).…”