Differential Proteome Analysis of Hybrid Bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) Under Fungal Stress (Arthrinium phaeospermum)

Abstract

In this study, TMT (tandem mass tag)-labeled quantitative protein technology combined with LC–MS/MS (liquid chromatography-mass spectrometry/mass spectrometry) was used to isolate and identify the proteins of the hybrid bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) and the bamboo inoculated with the pathogenic fungi Arthrinium phaeospermum. A total of 3320 unique peptide fragments were identified after inoculation with either A. phaeospermum or sterile water, and 1791 proteins were quantified. A total of 102 differentially expressed proteins were obtained, of which 66 differential proteins were upregulated and 36 downregulated in the treatment group. Annotation and enrichment analysis of these peptides and proteins using the GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) databases with bioinformatics software showed that the differentially expressed protein functional annotation items were mainly concentrated on biological processes and cell components. The LC–PRM/MS (liquid chromatography-parallel reaction monitoring/mass spectrometry) quantitative analysis technique was used to quantitatively analyze 11 differential candidate proteins obtained by TMT combined with LC–MS/MS. The up–down trend of 10 differential proteins in the PRM results was consistent with that of the TMT quantitative analysis. The coincidence rate of the two results was 91%, which confirmed the reliability of the proteomic results. Therefore, the differentially expressed proteins and signaling pathways discovered here may be the further concern for the bamboo-pathogen interaction studies.

Rhizosphere microorganisms of Crocus sativus as antagonists against pathogenic Fusarium oxysporum

“…Pathogen spore solution was then injected three times, 30 μL each time, with an interval of three days (day 3, 6, and 9), and cultured in a greenhouse at 28 ± 2°C to investigate the incidence rate of corm rot. The blank group was injected with PDB or NB, and the positive control group was injected with carbendazim, 12 corms per group, repeated three times ( Li et al., 2019 ).…”

Verification of the Interaction Target Protein of the Effector ApCE22 of Arthrinium phaeospermum in Bambusa pervariabilis × Dendrocalamopsis grandis

“…Most of the studies on the disease are about the pathogen A. phaeospermum , including the isolation and identification of the pathogen, the characteristics of disease occurrence, prevention methods, and other aspects of its physiological biology [ 7 , 8 , 9 ]. Moreover, studies on this pathogen also include bioinformatics such as whole-genome, transcriptome, protein analyses, and molecular biology analysis, such as the functional verification of virulence-related genes of the pathogen [ 10 , 11 , 12 ]. The research on the host B. pervariabilis × D. grandis has only involved proteome and transcriptome sequencing analysis after the plant has been infected by the pathogen [ 13 , 14 , 15 ].…”

Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis × Dendrocalamopsis grandis Shoot Blight

“…The differentially expressed proteins were mainly concentrated in biological processes, and the four differentially expressed proteins, sucrose synthase, adenosine triphosphate citrate synthase β chain protein 1, peroxidase, and phenylalanine ammonia lyase interacted significantly with a variety of proteins. The metabolites in response to pathogen infection are mainly concentrated in metabolic pathways, such as the citric acid cycle and phenylpropane biosynthesis [ 31 , 32 , 33 ]. However, this is only an independent analysis of the changes in the expression of host plant genes, proteins, and metabolites under specific pathological conditions, and there is a lack of research on the overall changes in gene expression in the process of pathogen–host interaction.…”