Differential protein input in the maternal diet alters the skeletal muscle transcriptome in fetal sheep

Sohel, Mahmodul Hasan; Akyüz, Bilal; Konca, Yusuf; Arslan, Korhan; Gürbulak, Kutlay; Abay, Murat; Kaliber, Mahmut; Çınar, Mehmet Ulaş

doi:10.1007/s00335-020-09851-3

Cited by 8 publications

(17 citation statements)

References 42 publications

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“…However, few studies have examined potential effects of maternal protein intake on the overall miRNA expression of fetal skeletal muscle. We have previously reported that maternal diets differing in protein content alter fetal skeletal transcriptome, and maternal high protein compared standard protein triggered pathways related to the immune system and disease in sheep skeletal muscle [18]. On the other hand, the mechanisms by which these diets affect fetal miRNAome in sheep skeletal muscle are not known.…”

Section: Introductionmentioning

confidence: 99%

“…Previous research has examined extreme undernutrition or overnutrition conditions to test the effects of maternal nutrition on fetal development in livestock and model animals, including fetal programming of muscle and fat tissues [12,17,18]. However, few studies have examined potential effects of maternal protein intake on the overall miRNA expression of fetal skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

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Effects of low and high maternal protein intake on fetal skeletal muscle miRNAome in sheep

Akyüz,

Sohel,

Konca

et al. 2024

Preprint

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Prenatal maternal feeding plays an important role in fetal development and has potential to cause long-lasting epigenetic modifications. MicroRNAs (miRNAs) are non-coding, single-stranded RNAs that serve as one epigenetic mechanism. Though miRNAs have crucial roles in fetal programming, growth, and development, there are limited data regarding maternal diet and sheep miRNA expression. Therefore, weanalyzed high and low maternal dietary protein for miRNA expression in sheep fetal longissimus dorsi. Pregnant ewes were fed an isoenergetic high-protein (HP, 160–270 g/day), low-protein (LP, 73–112 g/day), or standard protein diet (SP, 119–198 g/day) during pregnancy. miRNA expression profiles were evaluated using the Affymetrix GeneChip miRNA 4.0 Array. Twelve up-regulated, differentially expressed miRNAs (DE miRNAs) were identified targeting 65 genes. The oar-3957-5p miRNA was highly up-regulated in LP and SP compared to HP. Previous transcriptome analysis identified integrin and non-receptor protein tyrosine phosphatase genes targeted by miRNAs detected in the current experiment. A total of 28 GO terms and 10 pathway-based gene sets were significantly (padj &lt; 0.05) enriched in target genes. Most genes targeted by identified miRNAs are involved in immune and muscle disease pathways. Our study shows dietary protein intake during pregnancy affects fetal skeletal muscle epigenetics via miRNA expression.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of low and high maternal protein intake on fetal skeletal muscle miRNAome in sheep

Akyüz,

Sohel,

Konca

et al. 2024

Preprint

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“…MUN exerts its effect on the fetus by decreasing insulin sensitivity and promoting hypoxia [ 23 ]. Furthermore, MUN altered the gene expression profile in the fetal skeletal muscle tissue [ 24 , 25 , 26 , 27 , 28 ]. These studies have suggested that alteration of fetal skeletal muscle metabolism caused by MUN is crucial for the muscle development, however, the effect of MUN on fetal skeletal muscle metabolism has been poorly elucidated.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, difference in the result of MUN not only between animal species, breeds, or organs, but also between skeletal muscle types can be observed [ 24 , 30 ]. Comparison of experimental conditions among previous studies suggests that the effect of MUN on skeletal muscle gene expression varies between early and middle-to-late pregnancy of dams, between energy and protein restriction, and between levels of nutrition [ 24 , 25 , 26 , 27 , 28 ]. Notably, no significant phenotypic effect was observed in skeletal muscle mass or BW, in cases that the MUN period was limited to early-to-middle gestation [ 12 ] or that the nutritional level was compared between 70 and 100% or between 85 and 140% in requirement of energy for maintenance [ 30 , 31 ], even with significant effect on muscle fiber numbers and/or gene expression.…”

Section: Introductionmentioning

confidence: 99%

Maternal Undernutrition during Pregnancy Alters Amino Acid Metabolism and Gene Expression Associated with Energy Metabolism and Angiogenesis in Fetal Calf Muscle

et al. 2021

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To elucidate the mechanisms underlying maternal undernutrition (MUN)-induced fetal skeletal muscle growth impairment in cattle, the longissimus thoracis muscle of Japanese Black fetal calves at 8.5 months in utero was analyzed by an integrative approach with metabolomics and transcriptomics. The pregnant cows were fed on 60% (low-nutrition, LN) or 120% (high-nutrition, HN) of their overall nutritional requirement during gestation. MUN markedly decreased the bodyweight and muscle weight of the fetus. The levels of amino acids (AAs) and arginine-related metabolites including glutamine, GABA, and putrescine were higher in the LN group than those in the HN group. Metabolite set enrichment analysis revealed that the highly different metabolites were associated with the metabolic pathways of pyrimidine, glutathione, and AAs such as arginine and glutamate, suggesting that MUN resulted in AA accumulation rather than protein accumulation. The mRNA expression levels of energy metabolism-associated genes, such as PRKAA1, ANGPTL4, APLNR, CPT1B, NOS2, NOS3, UCP2, and glycolytic genes were lower in the LN group than in the HN group. The gene ontology/pathway analysis revealed that the downregulated genes in the LN group were associated with glucose metabolism, angiogenesis, HIF-1 signaling, PI3K-Akt signaling, pentose phosphate, and insulin signaling pathways. Thus, MUN altered the levels of AAs and expression of genes associated with energy expenditure, glucose homeostasis, and angiogenesis in the fetal muscle.

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“…For instance, it is currently accepted that the nutrition of parents widely affects the epigenetic modifications in progeny and therefore the expression of phenotypic characteristics in livestock (Murdoch et al 2016). Finally, transcriptomics is a well-integrated 'omics' area that comprises most of the research of nutrigenomics regarding ruminants (Osorio et al, 2017;Osorio et al, 2019;Sohel et al, 2020). Additional to the aforementioned three main areas, nutrigenomics also makes use of proteomics and metabolomics to create a total systems biology environment for a better understanding of nutrient-genome-phenotype interactions (Asmelash et al, 2018;Mondal and Ghosh, 2018;Müller and Kersten, 2003;Sales et al, 2014).…”

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confidence: 99%

Genome-wise engineering of ruminant nutrition – nutrigenomics: applications, challenges, and future perspectives – A review

Kızılaslan¹,

Arzık²,

Çınar

et al. 2022

Annals of Animal Science

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Use of genomic information in ruminant production systems can help relieve concerns related to food security and sustainability of production. Nutritional genomics (i.e., Nutrigenomics) is a field of research that is interested in all types of reciprocal interactions between nutrients and genomes of organisms, i.e., variable patterns of gene expression and effect of genetic variations on the nutritional environment. Devising a revolutionizing analytical approach to traditional ruminant nutrition research, the relatively novel area of ruminant nutrigenomics has several studies concerning different aspects of animal production systems. This paper aims to review the current nutrigenomics research in the frame of how nutrition of ruminants can be modified accounting for individual genetic backgrounds and gene/diet relationships behind productivity, quality, efficiency, disease resistance, fertility, and GHG emissions. Furthermore, current challenges facing ruminant nutrigenomics are evaluated and future directions for the novel area are strongly argued by this review.

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Differential protein input in the maternal diet alters the skeletal muscle transcriptome in fetal sheep

Cited by 8 publications

References 42 publications

Effects of low and high maternal protein intake on fetal skeletal muscle miRNAome in sheep

Effects of low and high maternal protein intake on fetal skeletal muscle miRNAome in sheep

Maternal Undernutrition during Pregnancy Alters Amino Acid Metabolism and Gene Expression Associated with Energy Metabolism and Angiogenesis in Fetal Calf Muscle

Genome-wise engineering of ruminant nutrition – nutrigenomics: applications, challenges, and future perspectives – A review

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