Differential protein expression analysis following olfactory learning in Apis cerana

Zhang, Lizhen; Wei, Yan; Wang, Zilong; Guo, Ya-Hui; Yao, Yi; Zhang, Shao Wu; Zeng, Zhen

doi:10.1007/s00359-015-1042-3

Cited by 11 publications

(9 citation statements)

References 56 publications

(65 reference statements)

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“…The consistency in the results with the mRNA expression by qRT-PCR analysis implies there is a real significance of BdorOBP2 in the ME perception by mature male flies. While a diverse pattern between mRNA and protein expression in two genes ( BdorOB19A and BdorPBP4 ) may reflect the lack of a direct relationship between mRNA and protein expression, it could also be attributed to post-transitional effects and/or other regulatory mechanisms, such as a lack of synchronization 42 – 44 .…”

Section: Discussionmentioning

confidence: 99%

BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)

Liu

Zhao

et al. 2017

Sci Rep

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Bactrocera dorsalis (Hendel) is a fruit-eating pest that causes substantial economic damage to the fresh produce industry in tropical and sub-tropical countries. Methyl eugenol (ME) is a powerful attractant for mature males of B. dorsalis, and has been widely used for detecting, luring and eradicating B. dorsalis populations worldwide. However, the molecular mechanism underlying the olfactory perception of ME remains largely unknown. Here, we analyzed the differential proteomics profiling of the antennae between ME-responsive and ME-non-responsive males by using isobaric tags for relative and absolute quantitation (iTRAQ). In total, 4622 proteins were identified, of which 277 proteins were significant differentially expressed, with 192 up-regulated and 85 down-regulated in responsive male antennae. Quantitative real-time PCR (qRT-PCR) analysis confirmed the authenticity and accuracy of the proteomic analysis. Based on the iTRAQ and qRT-PCR results, we found that the odorant-binding protein 2 (BdorOBP2) was abundantly expressed in responsive male antennae. Moreover, BdorOBP2 was significantly up-regulated by ME in male antennae. Mature males showed significantly greater taxis toward ME than did mature females. Silencing BdorOBP2 reduced mature males’ responsiveness to ME. These results indicate that BdorOBP2 may play an essential role in the molecular mechanism underlying B. dorsalis olfactory perception of ME.

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Section: Discussionmentioning

confidence: 99%

BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)

Liu

Zhao

et al. 2017

Sci Rep

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“…Thus, the sheer number and variety of mechanisms utilized by social insects for the transfer and exchange of information with nestmates makes them of particular interest for the investigation of social learning (Duskas, ). Like in Drosophila flies, serine/threonine‐protein kinases are also important for learning in social insects (Zhang et al, ). Learning in honeybees is facilitated by the activity of biogenic amines (Scheiner, Baumann, & Blenau, ), important mediators of neuronal signalling in the central and peripheral nervous systems of many invertebrates.…”

Section: Introductionmentioning

confidence: 99%

Tandem‐running and scouting behaviour are characterized by up‐regulation of learning and memory formation genes within the ant brain

et al. 2019

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Tandem‐running is a recruitment behaviour in ants that has been described as a form of teaching, where spatial information possessed by a leader is conveyed to following nestmates. Within Temnothorax ants, tandem‐running is used within a variety of contexts, from foraging and nest relocation to—in the case of slavemaking species—slave raiding. Here, we elucidate the transcriptomic basis of scouting, tandem‐leading and tandem‐following behaviours across two species with divergent lifestyles: the slavemaking Temnothorax americanus and its primary, nonparasitic host T. longispinosus. Analysis of gene expression data from brains revealed that only a small number of unique differentially expressed genes are responsible for scouting and tandem‐running. Comparison of orthologous genes between T. americanus and T. longispinosus suggests that tandem‐running is characterized by species‐specific patterns of gene usage. However, within both species, tandem‐leaders showed gene expression patterns median to those of scouts and tandem‐followers, which was expected, as leaders can be recruited from either of the other two behavioural states. Most importantly, a number of differentially expressed behavioural genes were found, with functions relating to learning and memory formation in other social and nonsocial insects. This includes a number of up‐regulated receptor genes such as a glutamate and dopamine receptor, as well as serine/threonine‐protein phosphatases and kinases. Learning and memory genes were specifically up‐regulated within scouts and tandem‐followers, not only reinforcing previous behavioural studies into how Temnothorax navigate novel environments and share information, but also providing insight into the molecular underpinnings of teaching and learning within social insects.

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“…Despite a small brain size, honeybees have been used as important models in neurobiology; these insects exhibit a wide repertoire of behaviors and a remarkable ability to learn [1]. Honeybees can identify odors and colors [2], as well the shapes and the relationships between objects such as sameness/differences [3,4], the order of the objects [5], numerical attributes associated with the objects [6], and relative positioning above/bellow [7].…”

Section: Introductionmentioning

confidence: 99%

“…Their outstanding olfactory memory is important in the search for food and may be developed in association with a series of various clues that are important in foraging [10,11]. These abilities appear to be important in ensuring that worker bees develop navigational routes, establish landmarks for these routes, and identify their forage sources [1,12,13].…”

Section: Introductionmentioning

confidence: 99%

Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex

Menegasso

Pratavieira

Fischer

et al. 2017

Journal of Proteomics

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The assay of proboscis extension reflex is frequently used to access honeybees' ability to correlate an unconditioned stimulus with a conditioned stimulus (such as an odor) to establish learning and memory acquisition. The reflex behavior of proboscis extension was associated with various conditioned stimuli, and the biochemical/genetic evaluation of the changes occurring in honeybee brains under these conditions reflect the synergistic effects of both insect manipulations (training to answer to an unconditioned stimulus and training to respond to a conditioned stimulus). Little or no information is available regarding the biochemical changes stimulated by an unconditioned stimulus alone, such as the proboscis extension reflex. The present investigation characterizes the proteomic changes occurring in the brains of honeybee workers submitted to proboscis extension reflex. A series of metabolic and cellular processes were identified to be related to the reflex of an unconditioned stimulus. This strategy may be reproduced to further understand the processes of learning and memory acquisition in honeybees.

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Differential protein expression analysis following olfactory learning in Apis cerana

Cited by 11 publications

References 56 publications

BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)

BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)

Tandem‐running and scouting behaviour are characterized by up‐regulation of learning and memory formation genes within the ant brain

Profiling the proteomics in honeybee worker brains submitted to the proboscis extension reflex

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