BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)

Abstract

Bactrocera dorsalis (Hendel) is a fruit-eating pest that causes substantial economic damage to the fresh produce industry in tropical and sub-tropical countries. Methyl eugenol (ME) is a powerful attractant for mature males of B. dorsalis, and has been widely used for detecting, luring and eradicating B. dorsalis populations worldwide. However, the molecular mechanism underlying the olfactory perception of ME remains largely unknown. Here, we analyzed the differential proteomics profiling of the antennae between ME-responsive and ME-non-responsive males by using isobaric tags for relative and absolute quantitation (iTRAQ). In total, 4622 proteins were identified, of which 277 proteins were significant differentially expressed, with 192 up-regulated and 85 down-regulated in responsive male antennae. Quantitative real-time PCR (qRT-PCR) analysis confirmed the authenticity and accuracy of the proteomic analysis. Based on the iTRAQ and qRT-PCR results, we found that the odorant-binding protein 2 (BdorOBP2) was abundantly expressed in responsive male antennae. Moreover, BdorOBP2 was significantly up-regulated by ME in male antennae. Mature males showed significantly greater taxis toward ME than did mature females. Silencing BdorOBP2 reduced mature males’ responsiveness to ME. These results indicate that BdorOBP2 may play an essential role in the molecular mechanism underlying B. dorsalis olfactory perception of ME.

Identification, characterization and expression analysis of transient receptor potential channel genes in the oriental fruit fly, Bactrocera dorsalis

“…Larvae were then transferred into small plastic boxes containing sand before pupation and pupae were kept at 27 °C ± 1 °C until adults emerged. Adult flies were fed another artificial diet comprising yeast extract and dry sugar mixed at a 1:1 ratio ( w /w) and housed in wooden cages measuring 35 cm by 35 cm by 35 cm [ 43 ].…”

Allyl‐2,6‐dimethoxyphenol, a female‐biased compound, is robustly attractive to conspecific males of Bactrocera dorsalis at close range

“…Newly emerged males and females were separated and reared at 27 AE 2°C, 60-70% r.h., and L12(1 200 lux):D12 h photoperiod until use. Adults were separated by sex within 4 days of emergence and reared with banana, water, and supplemented with sugar:yeast extract at 1:1 (wt/wt) (Liu et al, 2017). Research revealed that ca.…”

Glomerular Organization in the Antennal Lobe of the Oriental Fruit Fly Bactrocera dorsalis

“…In B. dorsalis , males can be strongly attracted to methyl eugenol (ME), but females do not show preference for this odor (Metcalf et al, 1975 ; Liu et al, 2017 ). This behavioral difference may be due to ME serving as a precursor for synthesis of the sex pheromone (Shelly et al, 2010 ; Tan and Nishida, 2012 ).…”

“…Methyl eugenol (ME) is an effective attractant to this insect, which has been widely used together with insecticides for monitoring and managing purposes (Fletcher, 1987 ; Shelly et al, 2004 ; Tan and Nishida, 2012 ). However, ME only attracts adult male flies (Tan and Nishida, 2012 ; Liu et al, 2017 ), limiting its power in monitoring the entire fly population. Additionally, ME is considered as a carcinogen to humans (Smith et al, 2002 ), calling for a need to develop safer and more efficient attractants.…”