Differential oxidative stress responses in castor semilooper, Achaea janata

Pavani, Ayinampudi; Chaitanya, R.K.; Chauhan, Vinod K.; Dasgupta, Anwesha; Dutta-Gupta, Aparna

doi:10.1016/j.jip.2015.10.002

Cited by 13 publications

(5 citation statements)

References 41 publications

(21 reference statements)

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“…ROS production is an essential component of plant stress responses, which are implicated in herbivory-induced responses in plants 39 , 43 . Evidence also showed that ROS-related enzymes such as peroxidase (POD), superoxide dismutase (SOD), glutathione S-transferases (GST) and ascorbate peroxidase (APX) could cause damage to insect cells and tissues 18 , 44 . Notably, APX, POD, and GST were identified to be potential targets by 2, 1, and 6 novel miRNAs, respectively, in this study (Fig.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in tea plant (Camellia sinensis L.)

Jeyaraj

Liu

Zhang

et al. 2017

Sci Rep

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The tea plant (Camellia sinensis L.) is vulnerable to the geometrid Ectropis oblique; although microRNAs (miRNAs) are important for plant growth, development and stress response, the function of miRNAs in the response of C. sinensis to stress from E. oblique is unclear. To identify E. oblique stress-responsive miRNAs and their target genes in tea plant, three small RNA libraries were constructed from leaves subjected to mechanical wounding (MW), geometrid attack (GA) and from healthy control (CK) leaves. Using high-throughput sequencing, 130 known miRNAs and 512 novel miRNAs were identified; of these, differential expression under GA stress was observed for 36 known and 139 novel miRNAs. Furthermore, 169 GA-responsive and 173 MW-responsive miRNAs were detected by miRNA microarray. The expression patterns of six GA-responsive miRNAs were validated by qRT-PCR. Several target genes for these miRNAs encode various transcription factors, including ethylene-responsive transcription factors and squamosa promoter-binding-like proteins, which suggests that these miRNAs may regulate stress-responsive transcriptional processes in tea plant. The present findings provide novel insights into miRNA-mediated regulatory mechanisms underlying the response to GA stress, and also offer valuable information for development of pest resistance using RNA interference-based strategies in tea plants.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in tea plant (Camellia sinensis L.)

Jeyaraj

Liu

Zhang

et al. 2017

Sci Rep

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“…The production of ROS is a prominent feature of innate immune responses. For example, after oral infection, insect hosts can heavily depend on an increase of ROS in the gut lumen, often in concert with a local up-regulation of antimicrobial peptides (AMPs) ( 6 , 18 – 20 ). Moreover, during bacterial antigen recognition by phagocytes, both ROS and nitrogen oxide are produced as a consequence of phagocytosis, in order to act as cytotoxins and second messengers ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

Cu,Zn Superoxide Dismutase Genes in Tribolium castaneum: Evolution, Molecular Characterisation, and Gene Expression during Immune Priming

Ferro

Corrà

et al. 2017

Front. Immunol.

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The production of reactive oxygen species (ROS) is a normal consequence of the aerobic cell metabolism. Despite their high and potentially detrimental reactivity with various biomolecules, the endogenous production of ROS is a vital part of physiological, immunological, and molecular processes that contribute to fitness. The role of ROS in host–parasite interactions is frequently defined by their contribution to innate immunity as effectors, promoting parasite death during infections. In vertebrates, ROS and antioxidant system enzymes, such as superoxide dismutase (SOD) are also involved in acquired immune memory, where they are responsible for T-cell signalling, activation, proliferation, and viability. Based on recent findings, ROS are now also assumed to play a role in immune priming, i.e., a form of memory in invertebrates. In this study, the potential involvement of Cu,Zn SODs in immunity of the red flour beetle Tribolium castaneum is described for the first time, applying an approach that combines an in silico gene characterisation with an in vivo immune priming experiment using the Gram-positive entomopathogen Bacillus thuringiensis. We identified an unusually high number of three different transcripts for extracellular SOD and found that priming leads to a fine-tuned modulation of SOD expression, highlighting the potential of physiological co-adaptations for immune phenotypes.

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“…Indeed, Hsp70 has been well-characterized as a stress-induced gene in the silk worm [29]. In the castor semi-looper, ( Achaea janata ), the exposure to Cry toxin, as considered as an oxidative stress, also induced the expression of several genes encoding antioxidant enzymes [30]. Based on these studies, it is likely that the up-regulation of Hsp70 in both S- and R-strain larvae fed transgenic Cry1Ab corn leaves may be related to the stress posed by Cry1Ab toxin and could be used as an indicator of Cry1Ab exposure in O. nubilalis .…”

Section: Resultsmentioning

confidence: 99%

Comparisons of Transcriptional Profiles of Gut Genes between Cry1Ab-Resistant and Susceptible Strains of Ostrinia nubilalis Revealed Genes Possibly Related to the Adaptation of Resistant Larvae to Transgenic Cry1Ab Corn

Yao

Zhu

et al. 2017

IJMS

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A microarray developed on the basis of 2895 unique transcripts from larval gut was used to compare gut gene expression profiles between a laboratory-selected Cry1Ab-resistant (R) strain and its isoline susceptible (S) strain of the European corn borer (Ostrinia nubilalis) after the larvae were fed the leaves of transgenic corn (MON810) expressing Cry1Ab or its non-transgenic isoline for 6 h. We revealed 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with expression ratio ≥2.0) in S-strain, but only 264 gut genes differentially expressed in R-strain after being fed transgenic corn leaves. Although the percentages of down-regulated genes among the total number of differentially expressed genes (50% in S-strain and 45% in R-strain) were similar between the R- and S-strains, the expression ratios of down-regulated genes were much higher in S-strain than in R-strain. We revealed that 17 and 9 significantly up- or down-regulated gut genes from S and R-strain, respectively, including serine proteases and aminopeptidases. These genes may be associated with Cry1Ab toxicity by degradation, binding, and cellular defense. Overall, our study suggests enhanced adaptation of Cry1Ab-resistant larvae on transgenic Cry1Ab corn as revealed by lower number and lower ratios of differentially expressed genes in R-strain than in S-strain of O. nubilalis.

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Differential oxidative stress responses in castor semilooper, Achaea janata

Cited by 13 publications

References 41 publications

Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in tea plant (Camellia sinensis L.)

Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in tea plant (Camellia sinensis L.)

Cu,Zn Superoxide Dismutase Genes in Tribolium castaneum: Evolution, Molecular Characterisation, and Gene Expression during Immune Priming

Comparisons of Transcriptional Profiles of Gut Genes between Cry1Ab-Resistant and Susceptible Strains of Ostrinia nubilalis Revealed Genes Possibly Related to the Adaptation of Resistant Larvae to Transgenic Cry1Ab Corn

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