Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in tea plant (Camellia sinensis L.)

Jeyaraj, Anburaj; Liu, Shengrui; Zhang, Xiao; Zhang, Ran; Shangguan, Mingzhu; Wei, Chaoling

doi:10.1038/s41598-017-13692-7

Cited by 63 publications

(24 citation statements)

References 77 publications

(97 reference statements)

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“…Bioinformatic identification of putative miRNA targets includes genes that encode NAC transcription factors and proteins in phytohormone signaling and antioxidant pathways as well as senescence-associated (SAG) proteins [ 36 ]. Similarly, herbivory of the tea plant Camellia sinensis by caterpillars of the geometrid moth Ectropis oblique result in the differential regulation of over 150 miRNA species [ 37 ], supporting the involvement of miRNA in plant defense responses against insect herbivores.…”

Section: Plant-insect Interactionsmentioning

confidence: 99%

miRNA-Mediated Interactions in and between Plants and Insects

Wong

Wang

et al. 2018

IJMS

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Our understanding of microRNA (miRNA) regulation of gene expression and protein translation, as a critical area of cellular regulation, has blossomed in the last two decades. Recently, it has become apparent that in plant-insect interactions, both plants and insects use miRNAs to regulate their biological processes, as well as co-opting each others’ miRNA systems. In this review article, we discuss the current paradigms of miRNA-mediated cellular regulation and provide examples of plant-insect interactions that utilize this regulation. Lastly, we discuss the potential biotechnological applications of utilizing miRNAs in agriculture.

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Section: Plant-insect Interactionsmentioning

confidence: 99%

miRNA-Mediated Interactions in and between Plants and Insects

Wong

Wang

et al. 2018

IJMS

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“…To date, 38,589 precursor miRNAs and 48,885 mature miRNAs from 271 species have been deposited in the miRNA database miRBase (Release 22, ) (Kozomara and Griffiths-Jones, 2014). However, several studies of miRNAs were previously reported (Zhang et al, 2014; Zheng et al, 2015; Liu et al, 2016; Jeyaraj et al, 2017). Tea genome sequence information was recently released by Wei et al (2018).…”

Section: Introductionmentioning

confidence: 99%

“…In tea plant, a fewer number of conserved and novel miRNAs were identified relative to other model plants (Zhu and Luo, 2013). Recently, a limited number of tea miRNAs were identified from responses to environmental stresses, such as drought, cold, and insect-induced stress (Zhang et al, 2014; Zheng et al, 2015; Liu et al, 2016; Jeyaraj et al, 2017). However, the miRNA-mediated gene regulatory networks that respond to the biotic C. gloeosporioides stress remain unexplored in tea plant.…”

Section: Introductionmentioning

confidence: 99%

Identification of Regulatory Networks of MicroRNAs and Their Targets in Response to Colletotrichum gloeosporioides in Tea Plant (Camellia sinensis L.)

Jeyaraj

Wang

et al. 2019

Front. Plant Sci.

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Anthracnose disease is caused by Colletotrichum gloeosporioides, and is common in leaves of the tea plant (Camellia sinensis). MicroRNAs (miRNAs) have been known as key modulators of gene expression in response to environmental stresses, disease resistance, defense responses, and plant immunity. However, the role of miRNAs in responses to C. gloeosporioides remains unexplored in tea plant. Therefore, in the present study, six miRNA sequencing data sets and two degradome data sets were generated from C. gloeosporioides-inoculated and control tea leaves. A total of 485 conserved and 761 novel miRNAs were identified. Of those, 239 known and 369 novel miRNAs exhibited significantly differential expression under C. gloeosporioides stress. One thousand one hundred thirty-four and 596 mRNAs were identified as targets of 389 conserved and 299 novel miRNAs by degradome analysis, respectively. Based on degradome analysis, most of the predicted targets are negatively correlated with their corresponding conserved and novel miRNAs. The expression levels of 12 miRNAs and their targets were validated by quantitative real-time PCR. A negative correlation between expression profiles of five miRNAs (PC-5p-80764_22, csn-miR160c, csn-miR828a, csn-miR164a, and csn-miR169e) and their targets (WRKY, ARF, MYB75, NAC, and NFY transcription factor) was observed. The predicted targets of five interesting miRNAs were further validated through 5’RLM-RACE. Furthermore, Gene Ontology and metabolism pathway analysis revealed that most of the target genes were involved in the regulation of auxin pathway, ROS scavenging pathway, salicylic acid mediated pathway, receptor kinases, and transcription factors for plant growth and development as well as stress responses in tea plant against C. gloeosporioides stress. This study enriches the resources of stress-responsive miRNAs and their targets in C. sinensis and thus provides novel insights into the miRNA-mediated regulatory mechanisms, which could contribute to the enhanced susceptibility of C. gloeosporioides in tea plant.

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“…In Cucumis melo, the resistant Vat + near isogenic lines and the susceptible Vat − exhibited distinct miRNA profiles under the infestation of Aphis gossypii 21 . Likewise, in Camellia sinensis infestation of Ectropis oblique resulted in the differential expression of 150 miRNAs, supporting the role of miRNA in plant-insect interactions 22 . To our knowledge, the first study on the roles of miRNAs in rice-BPH interactions was reported in 2017, revealing the differential miRNA responses in a resistant (BPH15 introgression line) and susceptible rice (recurrent parent 9311) in response to the infestation of BPH biotype 1 23 .…”

mentioning

confidence: 72%

Identification and analysis of miRNAs in IR56 rice in response to BPH infestations of different virulence levels

Nanda

Yuan

Lai

et al. 2020

Sci Rep

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Rice production and sustainability are challenged by its most dreadful pest, the brown planthopper (Nilaparvata lugens Stål, BPH). Therefore, the studies on rice-BPH interactions and their underlying mechanisms are of high interest. The rice ontogenetic defense, such as the role of microRNAs (miRNAs) has mostly been investigated against the pathogens, with only a few reports existing against the insect infestations. Thus, revealing the involvement of rice miRNAs in response to BPH infestations will be beneficial in understanding these complex interactions. In this study, the small RNA profiling of the IR56 rice in response to separate BPH infestations of varied virulence levels identified the BPH-responsive miRNAs and revealed the differential transcript abundance of several miRNAs during a compatible and incompatible rice-BPH interaction. The miRNA sequence analysis identified 218 known and 28 novel miRNAs distributed in 54 miRNA families. Additionally, 138 and 140 numbers of differentially expressed (DE) miRNAs were identified during the compatible and incompatible interaction, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed the target gene candidates of DE miRNAs (including osa-miR2871a-3p, osa-miR172a, osa-miR166a-5p, osa-miR2120, and osa-miR1859) that might be involved in the IR56 rice defense responses against BPH infestation. Conversely, osa-miR530-5p, osa-miR812s, osa-miR2118g, osa-miR156l-5p, osa-miR435 and two of the novel miRNAs, including novel_16 and novel_52 might negatively modulate the IR56 rice defense. The expressional validation of the selected miRNAs and their targets further supported the IR56 rice defense regulatory network. Based on our results, we have proposed a conceptual model depicting the miRNA defense regulatory network in the IR56 rice against BPH infestation. The findings from the study add further insights into the molecular mechanisms of rice-BPH interactions and will be helpful for the future researches.

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Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in tea plant (Camellia sinensis L.)

Cited by 63 publications

References 77 publications

miRNA-Mediated Interactions in and between Plants and Insects

miRNA-Mediated Interactions in and between Plants and Insects

Identification of Regulatory Networks of MicroRNAs and Their Targets in Response to Colletotrichum gloeosporioides in Tea Plant (Camellia sinensis L.)

Identification and analysis of miRNAs in IR56 rice in response to BPH infestations of different virulence levels

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