Differential ovarian expression of KiSS‐1 and GPR‐54 during the estrous cycle and photoperiod induced recrudescence in Siberian hamsters (<i>Phodopus sungorus</i>)

Shahed, Asha; Young, Kelly A.

doi:10.1002/mrd.20972

Cited by 73 publications

(51 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, in these studies it was observed that kiss1 mRNA expression occurs in a cyclic manner during the oestrous cycle with a significant increase at the preovulatory stage, which could be prevented by attenuating preovulatory LH surge and could be restored by hCG treatment [154]. Similar results were observed in indomethacintreated rats and photo-inhibited hamsters [155,157]. Additionally, recent studies on kiss1r heterozygous and knockout mice models further provide strong evidence in favour of the direct role of kisspeptins in the ovary by revealing that the loss of one kiss1r allele resulted in premature ovarian failure and loss of both kiss1r alleles blocked maturation of ovarian follicles and ovulation, which could not be rescued by gonadotrophins administration [159][160][161].…”

Section: Female Reproductive Tractsupporting

confidence: 57%

“…The initial evidence regarding the presence of kisspeptin and its receptor on the ovaries and uterus came from studies done on rats [153], which was later confirmed by immunohistochemical analysis performed in other species including humans [154][155][156][157][158]. Interestingly, in these studies it was observed that kiss1 mRNA expression occurs in a cyclic manner during the oestrous cycle with a significant increase at the preovulatory stage, which could be prevented by attenuating preovulatory LH surge and could be restored by hCG treatment [154].…”

Section: Female Reproductive Tractmentioning

confidence: 92%

See 1 more Smart Citation

Rola sygnalizacji kisspeptyny w osi podwzgórze–przysadka–nadnercza — aktualna perspektywa

Javed

Qamar

Sathyapalan

2015

Endokrynologia Polska

View full text Add to dashboard Cite

The discovery of kisspeptins in the recent past remoulded current understanding of the neuroendocrine axis relating to the regulation of human puberty and reproduction. Kisspeptins have been recognised to act upstream of GnRH and have been shown to play a vital role in the control of the hypothalamic-pituitary-gonadal axis via regulation of gonadotrophin secretion, onset of puberty, and control of fertility. KNDy (kisspeptin/neurokinin-B/dynorphin) neurons have been suggested to modulate GnRH pulsatile secretion, which is required to support reproductive function in both sexes. They have also been involved in mediating both positive and negative sex steroid feedback signals to GnRH neurons and serve as a vital connection between reproduction and metabolic status of the body. When kisspeptin is administered to healthy humans, and in patients with reproductive disorders, it strongly and directly stimulates GnRH and subsequent LH secretion and enhances LH pulse frequency. These observations suggest that kisspeptins are a potential novel therapeutic approach for treating disorders with either pathologically reduced or augmented gonadotrophins pulsatile secretion and is currently a focus of translational research. Kisspeptins have also been identified in several peripheral reproductive organs, indicating their role in modulation of ovarian function, embryo implantation, and placentation, but a great deal of work remains to be done to explore further in this regard, and the evidence is only available from studies done on animal models. In this review we will mainly focus on current available evidence related to the role of kisspeptins in controlling GnRH pulse frequency, specifically their role in puberty, fertility, and reproduction. We will also be appraising other factors that regulate the kiSS1/Kisspeptin/GPR-54 system. StreszczenieOdkrycie kisspeptyn, które miało miejsce całkiem niedawno, odmieniło obecne rozumienie osi neuroendokrynnej, związanej z regulacją okresu dojrzewania i rozrodu. Odkryto, że kisspeptyny działają przed GnRH i odgrywają istotną rolę w kontroli osi podwzgórze-przysadka-nadnercza poprzez regulację wydzielania gonadotropiny, rozpoczęcia okresu dojrzewania oraz kontroli płodności. Zasugerowano, że komórki KNDy (kisspeptyna/neurokinina-B/dynorfina) modulują pulsacyjne uwalnianie GnRH, wymagane, aby wspomagać funkcję rozrodczą u obu płci. Komórki te są również zaangażowane w przekazywanie zarówno pozytywnych, jak i negatywnych sygnałów hormonów płciowych do neuronów GnRH, a także stanowią kluczowe połączenie między reprodukcją i stanem metabolicznym ciała. Kiedy kisspeptyna jest podawana jednostkom zdrowym i pacjentom z zaburzeniami płodności, silnie i bezpośrednio stymuluje GnPH i dalsze uwalnianie LH oraz poprawia częstotliwość impulsów LH. Obserwacje te przedstawiają kisspeptyny jako nowe potencjalne terapeutyczne podejście w leczeniu zaburzeń patologicznie obniżonego lub zwiększonego pulsacyjnego uwalniania gonadotropin i obecnie stanowi główny punkt zainteresowania badań przekładaj...

show abstract

Section: Female Reproductive Tractsupporting

confidence: 57%

Section: Female Reproductive Tractmentioning

confidence: 92%

Rola sygnalizacji kisspeptyny w osi podwzgórze–przysadka–nadnercza — aktualna perspektywa

Javed

Qamar

Sathyapalan

2015

Endokrynologia Polska

View full text Add to dashboard Cite

show abstract

“…Total RNA from ovaries was isolated using Trizol LS reagent (Invitrogen Life Technologies, Carlsbad, CA) as per manufacturer protocol and as described previously (Shahed and Young, 2009). One μg of total RNA was first treated with DNase 1 (1 unit/μl) and incubated for 10 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Matrix metalloproteinase inhibition influences aspects of photoperiod stimulated ovarian recrudescence in Siberian hamsters

Shahed

Simmons

Featherstone

et al. 2015

General and Comparative Endocrinology

Self Cite

View full text Add to dashboard Cite

Blocking matrix metalloproteinase (MMP) activity in vivo with inhibitor GM6001 impedes photostimulated ovarian recrudescence in photoregressed Siberian hamsters. Since direct and indirect effects of MMPs influence a myriad of ovarian functions, we investigated the effect of in vivo MMP inhibition during recrudescence on ovarian mRNA expression of steroidogenic acute regulatory protein (StAR), 3β-hy-droxysteroid dehydrogenase (3β-HSD), Cyp19a1 aromatase, epidermal growth factor receptor (EGFR), amphiregulin (Areg), estrogen receptors (Esr1 and Esr2), tissue inhibitors of MMPs (TIMP-1,-2,-3), proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor A (VEGFA), its receptor VEGFR-2, and angiopoietin-2 (Ang-2). Female Siberian hamsters were randomly assigned to one of four photoperiod groups: stimulatory long (LD) or inhibitory short (SD) photoperiods, or transferred from SD to LD for 2 weeks (post-transfer, PT). Half of the PT hamsters were injected (ip) daily with GM6001 (PTG). SD exposure reduced ovarian StAR, 3β-HSD, Cyp19a1, Esr1, Esr2, TIMPs 2–3, PCNA, VEGFR-2 and Ang-2 mRNA expression (p < 0.05), and 2 weeks of photostimulation restored mRNA expression of 3β-HSD and PCNA and increased Areg and VEGFA mRNA expression in the PT group. GM6001 treatment during photostimulation (PTG) increased TIMP-1, -2 and -3 and PCNA mRNA, but inhibited Areg mRNA expression compared to PT. Neither photoperiod nor GM6001 altered EGFR expression. Results of this study suggest that in vivo inhibition of MMP activity by GM6001 may impede ovarian recrudescence, particularly follicular growth, in two ways: (1) directly by partially inhibiting the release of EGFR ligands like Areg, thereby potentially affecting EGFR activation and its downstream pathway, and (2) indirectly by its effect on TIMPs which themselves can affect proliferation, angiogenesis and follicular growth.

show abstract

“…Total RNA was isolated from ovarian tissue with Trizol (Invitrogen, Carlsbad, CA) and cDNA was synthesized exactly as described before (Shahed & Young, 2009). Primers were designed using Siberian hamster sequences (PCR products extracted and sequenced as described Shahed & Young, 2009) and OligoPerfect Designer software (Invitrogen) using the MX3000 cycler manual design criteria (Stratagene Corp., Cedar Creek, TX). Primer sequences used were: MMP-2 (forward) ATG ATG TCA GCT TCC CCA TC; MMP-2 (reverse) ACC TGG ACC CTG AAA CAG TG ; MMP-9 (forward) ACT TTG GAA ACG CAA ATG GT ; MMP-9 (reverse) AGT CTC TCA CTG GGG CAG AA .…”

Section: Methodsmentioning

confidence: 99%

Inhibition of matrix metalloproteinases in Siberian hamsters impedes photostimulated recrudescence of ovaries

Whited¹,

Shahed²,

McMichael³

et al. 2010

Reproduction

Self Cite

View full text Add to dashboard Cite

Exposure of Siberian hamsters to short photoperiod for 14wks induces ovarian regression. Subsequent transfer to long photoperiod restores ovarian function, and 2 weeks of photostimulation increases plasma estradiol, antral follicles and corpora lutea. Because tissue remodeling involved with photostimulated ovarian recrudescence is associated with differential expression of matrix metalloproteinases (MMPs), we hypothesized that inhibiting MMP activity using a broad-spectrum in vivo MMP-inhibitor, GM6001, would curtail recrudescence. One group of hamsters was placed in long-days (LD;16:8) for 16wks. Another group was placed in inhibitory short-days (SD;8:16) for 14wks. A third group was placed in SD for 14wks and transferred to LD for 2wks to stimulate recrudescence. During weeks 14–16 animals were either not treated, or treated daily with intraperitoneal injections of GM6001(20mg/kg), or vehicle(DMSO). GM6001 reduced gelatinase activity and decreased immunohistochemical staining for MMPs-1, -2 and -3 compared to vehicle. No differences between controls, vehicle, or GM6001 treatment were observed among LD animals, despite a trend towards reduction in corpora lutea and estradiol with GM6001. Whereas SD reduced ovarian function, photostimulation of transferred controls increased uterine mass, plasma estradiol, appearance of antral follicles and corpora lutea. With GM6001 treatment, photostimulation failed to increase uterine mass, plasma estradiol, antral follicles or corpora lutea. These data show, for the first time, that in vivo GM6001 administration inhibits MMP activity in hamster ovaries during photostimulation, and indicate that this inhibition may impede photostimulated recrudescence of ovaries. This study suggests an intriguing link between MMP activity and return to ovarian function during photostimulated recrudescence.

show abstract

Differential ovarian expression of KiSS‐1 and GPR‐54 during the estrous cycle and photoperiod induced recrudescence in Siberian hamsters (Phodopus sungorus)

Cited by 73 publications

References 55 publications

Rola sygnalizacji kisspeptyny w osi podwzgórze–przysadka–nadnercza — aktualna perspektywa

Rola sygnalizacji kisspeptyny w osi podwzgórze–przysadka–nadnercza — aktualna perspektywa

Matrix metalloproteinase inhibition influences aspects of photoperiod stimulated ovarian recrudescence in Siberian hamsters

Inhibition of matrix metalloproteinases in Siberian hamsters impedes photostimulated recrudescence of ovaries

Contact Info

Product

Resources

About