Differential measurement of monomeric and polymeric IgA by one-directional immunoelectrodiffusion

Meillet, D; Tallet, Frank; Gobert, J.G.; Savel, J; Yonger, J; Raichvarg, D

doi:10.1016/0009-8981(86)90095-1

Cited by 5 publications

(4 citation statements)

References 5 publications

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“…The reference measure (often per gram feces) will be profoundly affected by fecal water content, and intestinal transit is also relevant-i.e., in relation to the time available within the lumen of the gastrointestinal tract for molecules of interest to be destroyed by digestive enzymes and bacterial proteases. Whereas the estimated intestinal production oflgA in humans is 40 mg/kg body weight/day (15), reports offecaligA content are much lower, e.g., a mean daily output of 58.5 mg (range, 6-173 mg/day) in 16 healthy adults (16); we find that the IgA content of fecal fluid is 10-fold lower than that of gut perfusate (unpublished). Furthermore, some of the substances in feces (and also in intestinal fluids such as WGLF) may be derived from plasma that has leaked into the gut lumen through ulcerated or inflamed sections of intestinal mucosa.…”

Section: Cytokine Studiesmentioning

confidence: 51%

Use of Whole‐Gut Lavage to Measure Intestinal Immunity in Healthy Sierra Leonean Children

Hodges,

Kingstone,

Brydon

et al. 1994

J. pediatr. gastroenterol. nutr.

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SummaryIn view of the potential roles of intestinal immunodeficiency and hypersensitivity in the infection/diarrhea/malnutrition cycle, we need a safe and ethical method to study intestinal immunity of children in the developing world. Work in adults has shown that the fluid obtained by whole‐gut lavage (WGLF), essentially a gut perfusate, can be used to assess intestinal immunity, inflammation, and gut losses of protein and blood. Gut lavage was successfully performed in 24 of 25 “normal” children aged 6–9 years, from Freetown, Sierra Leone, with parental informed consent. WGLF was treated with protease inhibitors, stored at −20°C, and transferred to Edinburgh for laboratory studies. These showed that no child had occult blood loss but four had evidence of protein‐losing enteropathy. Compared with values for Scottish adults, WGLF from the Sierra Leonean children had significantly higher concentrations of IgA and IgM and of IgA and IgM antibodies to dietary antigens and to Salmonella typhi lipopolysaccharide. In three children, very low levels of IgA and IgA antibody were present: Two of these were the only cases with detectable sIL2R in lavage fluid, indirect evidence of intestinal T cell activation; tumor necrosis factor was not detectable. Substantial information on childrens' intestinal immunity can be obtained by the method described.

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Section: Cytokine Studiesmentioning

confidence: 51%

Use of Whole‐Gut Lavage to Measure Intestinal Immunity in Healthy Sierra Leonean Children

Hodges,

Kingstone,

Brydon

et al. 1994

J. pediatr. gastroenterol. nutr.

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“…The proportions of monomeric and polymeric forms of IgA in various mucosal secretions, including saliva, vary considerably. Indeed monomeric IgA ranges from 14% in colostrum [27]. 30"';.…”

Section: Discussionmentioning

confidence: 99%

“…Total IgA {t-IgA), polymeric (p-IgA) and monomeric IgA (mIgA) were assayed using a modified one-dimensional immunoeleetrophoretic method [27]. Briefly, electrophoresis was run for 20 h in veronal buffer at a potential of 150 V in a 2% agarose-6% poiyacrylamide gel in the presence of a gel barrier (20% poiyacrylamide) which blocks polymeric IgA {p-lgA).…”

Section: Study Ofthe Monomer I Total Iga and Polymer I Total Iga Ratimentioning

confidence: 99%

Secretory IgA are elevated in both saliva and serum of patients with various types of primary glomerulonephritis

Rostoker

Terzidis

Petit-Phar

et al. 1992

Clinical and Experimental Immunology

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SUMMARYSecretory immunoglobulin A (IgA) was determined by means of an enzyme-linked immunosorbent assay (using as capture antibody an MoAb specifie for secretory component) in saliva and serum from 46 patients with IgA mesangial nephritis (IgAGN). 36 with an idiopathic nephrotic syndrome (INS), 30 with an idiopathic membranous nephropathy (MGN) and 40 healthy controls. Secretory IgA levels were elevated in both saliva and serum of patients with primary glomerulonephrilis (/'<0-05; Mann-Whitney test) regardless of the histological type ofthe primary glomerulonephritis. Salivary IgA 1 and IgA2 levels were inereased in the saliva of patients with IgAGN. INS and MGN {P< 005; Mann-Whitney test)-The monomeric/total IgA ratio, and interferon-gamma and soluble IL-2 receptor levels, in saliva did not differ between the patients and controls (P>005; MannWhitney test). We conclude that the mucosal immune systetn is activated in forms ofglomerulonephritis other than IgAGN.

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“…IgA immune complexes were measured according to Imai et al [22] after 14% PEG 6000 precipitation (7% final concentration) by a specific sandwich ELISA. Polymeric IgA was assayed by an immunoelectrophoretic method according to Mcillct et al [23], while serum secretory IgA was measured by an ELISA [24]. Rheumatoid factors of the IgA class were detected using an indirect ELISA according to Sinico et al [25] and Schena ct al.…”

Section: Methodsmentioning

confidence: 99%

Immunomodulation with Low-Dose Immunoglobulins for Moderate IgA Nephropathy and Henoch-Schönlein Purpura

Rostoker

Desvaux-Belghiti²,

Pilatte

et al. 1995

Nephron

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Recently, our group has shown that a 3-month course of intravenous immunoglobulin (2 g/kg/monthly) followed by 6 months of intramuscular immunoglobulins (IMIG, 16.5%, 0.35 ml/kg every 15 days) was able to slow or to stop the decline in the glomerular filtration rate, to reduce proteinuria, hematuria, leukocyturia and the histological index of activity on renal biopsy in patients with severe forms of IgA nephropathy (IGAN) and Henoch-Schönlein purpura (HSP). The aim of this open prospective trial was to evaluate the efficacy and safety of low-dose immunoglobulin therapy in moderate IGAN and HSP with permanent proteinuria. Fourteen patients with moderate IGAN [idiopathic IGAN: n= 11; chronic idiopathic HSP: n = 3] and permanent albuminuria were treated with polyvalent IMIG (16.5%) for 9 months (0.35 ml/kg once a week for 1 month, followed by 0.35 ml/kg every 15 days for a further 8 months). Eligibility criteria in the study were Lee histological stage I, II or III, albuminuria between 300 and 2,000 mg/ day and a glomerular filtration rate > 70 ml/min/1.73 m². IMIG were well tolerated and only 1 patient withdrew from the trial. No viral, renal or immunological side effects were observed. IMIG induced a significant decrease in albuminuria as well as in the histological activity index in the 11 cases in which a follow-up biopsy was performed. There was also a decrease in serum IgA, serum β₂-microglobulin and IgA immune complex levels, and an increase in serum IgG₁ levels. Twelve of the 13 evaluable patients improved during treatment. Systemic symptoms disappeared after 2 months of starting IMIG in the 3 HSP patients. IMIG therapy for IGAN only appeared to be suspensive, since its withdrawal was followed by relapse. We conclude that IMIG may be an effective immunomodulatory treatment of IGAN and HSP, although prospective controlled trials are required to confirm these preliminary results.

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Differential measurement of monomeric and polymeric IgA by one-directional immunoelectrodiffusion

Cited by 5 publications

References 5 publications

Use of Whole‐Gut Lavage to Measure Intestinal Immunity in Healthy Sierra Leonean Children

Use of Whole‐Gut Lavage to Measure Intestinal Immunity in Healthy Sierra Leonean Children

Secretory IgA are elevated in both saliva and serum of patients with various types of primary glomerulonephritis

Immunomodulation with Low-Dose Immunoglobulins for Moderate IgA Nephropathy and Henoch-Schönlein Purpura

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