Differential Kinetics and Distribution of Antibodies in Serum and Nasal and Vaginal Secretions after Nasal and Oral Vaccination of Humans

Rudin, Anna; Johansson, Eva-Liz; Bergquist, Charlotta; Holmgren, Jan

doi:10.1128/iai.66.7.3390-3396.1998

Cited by 114 publications

(34 citation statements)

References 36 publications

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“…It has been suggested that i.n. immunization, in general, is efficient for stimulating systemic immunity (4) and elicits an antibody response with later onset but of longer duration than the oral route (34).…”

Section: Discussionmentioning

confidence: 99%

Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with MutantEscherichia coliHeat-Labile Toxin in Gnotobiotic Pigs

Yuan

Iosef

Azevedo

et al. 2001

J Virol

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Two combined rotavirus vaccination regimens were evaluated in a gnotobiotic pig model of rotavirus infection and disease and were compared to previously tested rotavirus vaccination regimens. The first (AttHRV/VLP2؋) involved oral inoculation with one dose of attenuated (Att) Wa human rotavirus (HRV), followed by two intranasal (i.n.) doses of a rotavirus-like particle (2/6-VLPs) vaccine derived from Wa (VP6) and bovine RF (VP2) rotavirus strains. The 2/6-VLPs were coadministered with a mutant Escherichia coli heat-labile toxin, LT-R192G (mLT) adjuvant. For the second regimen (VLP2؋/AttHRV), two i.n. doses of 2/6-VLPs؉mLT were given, followed by one oral dose of attenuated Wa HRV. To compare the protective efficacy and immune responses induced by the combined vaccine regimens with individual rotavirus vaccine regimens, we included in the experiments the following vaccine groups: one oral dose of attenuated Wa HRV (AttHRV1؋ and Mock2؋/AttHRV, respectively), three oral doses of attenuated Wa HRV (AttHRV3؋), three i.n. doses of 2/6-VLPs plus mLT (VLP3؋), three i.n. doses of purified double-layered inactivated Wa HRV plus mLT (InactHRV3؋), mLT alone, and mock-inoculated pigs. The isotype, magnitude, and tissue distribution of antibody-secreting cells (ASCs) in the intestinal and systemic lymphoid tissues were evaluated using an enzyme-linked immunospot assay. The AttHRV/VLP2؋ regimen stimulated the highest mean numbers of intestinal immunoglobulin A (IgA) ASCs prechallenge among all vaccine groups. This regimen induced partial protection against virus shedding (58%) and diarrhea (44%) upon challenge of pigs with virulent Wa HRV. The reverse VLP2؋/AttHRV regimen was less efficacious than the AttHRV/VLP2؋ regimen in inducing IgA ASC responses and protection against diarrhea (25% protection rate) but was more efficacious than VLP3؋ or InactHRV3؋ (no protection). In conclusion, the AttHRV/VLP2؋ vaccination regimen stimulated the strongest B-cell responses in the intestinal mucosal immune system at challenge and conferred a moderately high protection rate against rotavirus disease, indicating that priming of the mucosal inductive site at the portal of natural infection with a replicating vaccine, followed by boosting with a nonreplicating vaccine at a second mucosal inductive site, may be a highly effective approach to stimulate the mucosal immune system and induce protective immunity against various mucosal pathogens.

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Section: Discussionmentioning

confidence: 99%

Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with MutantEscherichia coliHeat-Labile Toxin in Gnotobiotic Pigs

Yuan

Iosef

Azevedo

et al. 2001

J Virol

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“…Briefly, analysis was done using several arbitrarily assigned cut points, comparing HPV-16 positive with HPV-16 negative women's OD values and a cut point chosen with maximum specificity. Total Ig estimations on CVR specimens were conducted as described by Rudin et al [1998] using goat anti-human IgA, alpha chain specific or IgG, specific for gamma-chains (Dako, Carpinteria, CA) and standards, polyclonal human IgA or IgG (Dako). CVR antibody results were standardized to total the Ig to account for antibody changes with the menstrual cycle.…”

Section: Elisamentioning

confidence: 99%

HIV‐1 seroconversion promotes rapid changes in cervical human papillomavirus (HPV) prevalence and HPV‐16 antibodies in female sex workers

Marais

Carrara

Ramjee

et al. 2008

Journal of Medical Virology

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The extent to which human immunodeficiency virus (HIV-1) infection impacts on the ability to mount an effective immune response to HPV is unknown, but is relevant in planning HPV vaccine strategies for HIV-1 infected individuals. This longitudinal study investigated changes shortly after HIV-1 seroconversion on cervical HPV types and HPV-16 antibody responses in serum and at the cervix of female sex workers. Typing of HPV DNA from cervical cells was done prior to HIV-1 seroconversion and within 1 year and greater than 2 years after HIV-1 seroconversion. Antibody determinations on serum and cervico-vaginal rinse samples were by HPV-16 virus-like particle-based, enzyme-linked immunosorbent assay. Of 104 women tested, 40 (38.4%) became HIV-1 seropositive (HIV-positive) during the course of the study. Shortly after HIV-1 seroconversion a significant increase in multiple (>1) HPV infection (OR 4.0, 95% CI 1.3-11.9) was observed compared with HIV-1 seronegative (HIV-negative) women and certain changes in HPV type infection. HIV-1 seroconversion resulted in a reduced prevalence of serum HPV-16 IgA and cervico-vaginal IgA and IgG but an increased prevalence of serum HPV-16 IgG. All HIV-positive women had been exposed to HPV-16 as all displayed serum HPV-16 IgG. Serum HPV-16 responses were maintained at a high magnitude in the presence of HPV-16 infection irrespective of HIV infection, but decreased in the absence of HPV-16 infection. In conclusion, HIV-1 seroconversion in sex workers rapidly increased cervical HPV infection and caused a reduced ability to produce cervical HPV-16 antibodies but a continued ability to generate serum IgG antibodies.

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“…Nasal vaccination is often superior to oral application and might result in a general mucosal immune reaction [22]. Therefore, we compared oral and nasal application of Salmonella harbouring an eukaryotic expression plasmid encoding L gal.…”

Section: Induction Of Systemic and Local Immune Responses After Oral mentioning

confidence: 99%

Oral delivery of DNA vaccines using attenuated Salmonella typhimurium as carrier

Darji¹

2000

FEMS Immunology and Medical Microbiology

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The efficacious delivery of eukaryotic expression plasmids to inductive cells of the immune system constitutes a key prerequisite for the generation of effective DNA vaccines. Here, we have explored the use of bacteria as vehicles to orally deliver expression plasmids. Attenuated Salmonella typhimurium aroA harbouring eukaryotic expression plasmids that encoded virulence factors of Listeria monocytogenes were administered orally to BALB/c mice. Strong cytotoxic and helper T cell responses as well as antibody production were elicited even after a single administration. Mice immunised four times with Salmonella that carried a eukaryotic expression plasmid encoding the secretory listerial protein listeriolysin were protected against a subsequent lethal challenge with this pathogen. A single dose was already partially protective. The efficiency of this vaccination procedure was due to transfer of the expression plasmid from the bacterial carrier to the mammalian host. Evidence for such an event could be obtained in vivo and in vitro. Expression of the desired antigen in various lymphoid tissues was already detectable 1 day after administration of the DNA vaccine and persisted for at least 1 month in spleen and mesenteric lymph nodes. Induction of cytotoxic and helper T cell responses was observed in all mouse strains tested including outbred strains whereas antibodies were mainly detected in BALB/c. Furthermore, we could show that immunogenicity could be improved by increasing the invasiveness of the bacterial carrier.

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Differential Kinetics and Distribution of Antibodies in Serum and Nasal and Vaginal Secretions after Nasal and Oral Vaccination of Humans

Cited by 114 publications

References 36 publications

Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with MutantEscherichia coliHeat-Labile Toxin in Gnotobiotic Pigs

Protective Immunity and Antibody-Secreting Cell Responses Elicited by Combined Oral Attenuated Wa Human Rotavirus and Intranasal Wa 2/6-VLPs with MutantEscherichia coliHeat-Labile Toxin in Gnotobiotic Pigs

HIV‐1 seroconversion promotes rapid changes in cervical human papillomavirus (HPV) prevalence and HPV‐16 antibodies in female sex workers

Oral delivery of DNA vaccines using attenuated Salmonella typhimurium as carrier

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