Differential involvement of rat sperm choline glycerophospholipids and sphingomyelin in capacitation and the acrosomal reaction

Zanetti, Samanta Romina; Monclus, Maria; Rensetti, Daniel E.; Fornés, Miguel Walter; Aveldaño, Marta I.

doi:10.1016/j.biochi.2010.08.015

Cited by 27 publications

(35 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…chromaffin cells (12) and neurons (88,89). Given that diverse exocytic stimuli induce changes in the sperm lipid profile (90,91), and in particular that PIP 2 (33) and PIP 3 levels (92) are increased, we hypothesize that both lipids contribute to ARF6-GEs binding to membranes, triggering ARF6 activation and the signal transduction cascade described. Further studies are needed, however, to assess the presence of GEFs and GAPs for ARF6 in human spermatozoa.…”

Section: Discussionmentioning

confidence: 96%

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

Pelletán

Suhaiman

Vaquer

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Sperm acrosomal exocytosis requires GTPases, SNAREs, and a complex lipid signaling. Results: Exocytic stimuli promote ARF6 activation, which accomplishes exocytosis by stimulating PLC and Rab3A. Conclusion: ARF6 induces acrosome calcium efflux and assembles the fusion machinery leading to membrane fusion. Significance: This study explores a novel molecular link between ARF6, PLC, and Rab3A and provides insight into the molecular mechanisms of exocytosis and reproduction.

show abstract

Section: Discussionmentioning

confidence: 96%

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

Pelletán

Suhaiman

Vaquer

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Its release to incubation media that contain a suitable cholesterol acceptor (e.g., albumin) during this process is one of the widely accepted prerequisites for sperm capacitation to proceed [23][24][25]. Zanetti et al [14] recently showed that during incubation of rat spermatozoa in conditions that lead to their capacitation, the release of cholesterol concurs with a massive hydrolysis of sperm Gpl, with release of plenty of free fatty acids to an albumin-containing medium.…”

Section: Discussionmentioning

confidence: 99%

“…After incubation in conditions that lead to sperm capacitation, a significant hydrolysis of ChoGpl occurs that is accompanied by the release of plenty of 22:4n-9 and 22:5n-6 to the media [14], suggesting that plasmalogens participate in this important process. The biological significance that the regionalization of major plasmalogens may have in sperm physiology certainly demands further studies.…”

Section: Discussionmentioning

confidence: 99%

“…It was recently shown [14] that incubation of rat spermatozoa in conditions that evoke this calcium-dependent reaction results in the massive hydrolysis of both (n-and 2-OH VLCPUFA-containing) species of CerPCho, and the concomitant increase in the production of the corresponding species of Cer. The present results, locating these sphingomyelins to the head, are consistent with the acrosomal reaction being a function that exclusively occurs in this sperm area.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, using divalent cation-free media, most of the n-VLCPUFA-rich species of CerPCho that can be recovered from spermatozoa were found to be located to the head, not only in rat but in bull spermatozoa [13]. It was then intriguing that whole rat spermatozoa contained relatively high levels of Cer with 2-OH VLCPUFA [14], even if isolated in divalent cation-free media [5].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Uneven Distribution of Ceramides, Sphingomyelins and Glycerophospholipids Between Heads and Tails of Rat Spermatozoa

Oresti

Luquez

Furland

et al. 2011

Lipids

Self Cite

View full text Add to dashboard Cite

Previous work showed that rat germ cells and spermatozoa contain ceramides and sphingomyelins with high proportions of nonhydroxy and 2-hydroxy (2-OH) polyunsaturated fatty acids (PUFA) with very long chains (VLCPUFA). The aim of this study was to assess how these lipids are distributed between the heads and tails of mature spermatozoa in comparison with other membrane lipid classes. In addition to quantitative differences due to the fact that these gametes have a long, voluminous tail and a minute head, several compositional dissimilarities emerged between these two regions. The total cholesterol/total phospholipid ratio, the choline/ethanolamine glycerophospholipid (ChoGpl/EtnGpl) ratio, and the proportion of plasmalogens within these two classes, were much larger in the head than in the tail. Whereas EtnGpl was rich in 22:5n-6 in both regions, ChoGpl had plenty of 22:4n-9, especially in the heads. An important proportion of the head EtnGpl- 22:5n-6 and ChoGpl 22:4n-9 was in plasmenyl- (rather than in phosphatidyl-) subclasses. The heads concentrated all of the sphingomyelin species with nonhydroxy- and 2-OH VLCPUFA, and the tails most of the saturated fatty acids that are present in total sperm sphingomyelin. Unexpectedly, virtually all of the abundant spermatozoal ceramides, predominantly made up by species with 2-OH VLCPUFA, was located in the tail. The fact that intact rat spermatozoa constitutively have much more VLCPUFA-containing ceramide than sphingomyelin is explained by the present findings, since the former are mostly lipids of the large tail while the latter mostly collect in the small head.

show abstract

Sphingolipids controlling ciliary and microvillar function

2020

View full text Add to dashboard Cite

Cilia and microvilli are membrane protrusions that extend from the surface of many different mammalian cell types. Motile cilia or flagella are only found on specialized cells, where they control cell movement or the generation of fluid flow, whereas immotile primary cilia protrude from the surface of almost every mammalian cell to detect and transduce extracellular signals. Despite these differences, all cilia consist of a microtubule core called the axoneme. Microvilli instead contain bundled linear actin filaments and are mainly localized on epithelial cells, where they modulate the absorption of nutrients. Cilia and microvilli constitute subcellular compartments with distinctive lipid and protein repertoires and specialized functions. Here, we summarize the role of sphingolipids in defining the identity and controlling the function of cilia and microvilli in mammalian cells.

show abstract

Differential involvement of rat sperm choline glycerophospholipids and sphingomyelin in capacitation and the acrosomal reaction

Cited by 27 publications

References 42 publications

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

ADP Ribosylation Factor 6 (ARF6) Promotes Acrosomal Exocytosis by Modulating Lipid Turnover and Rab3A Activation

Uneven Distribution of Ceramides, Sphingomyelins and Glycerophospholipids Between Heads and Tails of Rat Spermatozoa

Sphingolipids controlling ciliary and microvillar function

Contact Info

Product

Resources

About