Sphingolipids controlling ciliary and microvillar function

Kaiser, Fabian; Huebecker, Mylene; Wachten, Dagmar

doi:10.1002/1873-3468.13816

Cited by 17 publications

(18 citation statements)

References 144 publications

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“…PIP 2 was also found to accumulate in microvilli ( 30 ). But comprehensive analysis of lipids in microvilli has not been performed to date ( 38 ). Therefore, it is unclear what is the content of PIP 2 , sphingolipids and other lipids (e.g., cholesterol) in these structures.…”

Section: Clustering Of Sphingolipids and Phosphoinositides Triggers Mmentioning

confidence: 99%

Role of Lipids in Morphogenesis of T-Cell Microvilli

Cebecauer

2021

Front. Immunol.

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T cells communicate with the environment via surface receptors. Cooperation of surface receptors regulates T-cell responses to diverse stimuli. Recently, finger-like membrane protrusions, microvilli, have been demonstrated to play a role in the organization of receptors and, hence, T-cell activation. However, little is known about the morphogenesis of dynamic microvilli, especially in the cells of immune system. In this review, I focus on the potential role of lipids and lipid domains in morphogenesis of microvilli. Discussed is the option that clustering of sphingolipids with phosphoinositides at the plasma membrane results in dimpling (curved) domains. Such domains can attract phosphoinositide-binding proteins and stimulate actin cytoskeleton reorganization. This process triggers cortical actin opening and bundling of actin fibres to support the growing of microvilli. Critical regulators of microvilli morphogenesis in T cells are unknown. At the end, I suggest several candidates with a potential to organize proteins and lipids in these structures.

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Section: Clustering Of Sphingolipids and Phosphoinositides Triggers Mmentioning

confidence: 99%

Role of Lipids in Morphogenesis of T-Cell Microvilli

Cebecauer

2021

Front. Immunol.

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“…The copyright holder for this preprint (which this version posted July 16, 2021. ; https://doi.org/10.1101/2021.07.16.452691 doi: bioRxiv preprint biosynthesis process and is important in regulating cilia functions and formations 43 . The unknown cluster interesting had GO terms associated with cell proliferation (positive/negative regulations of proliferation), extracellular matrix (extracellular matrix organization, collagen fibril organization) and angiogenesis-related (angiogenesis, blood vessel development, sprouting angiogenesis) biological pathways.…”

Section: Generation Of Mature Adult-like Airway Epithelial Tissuementioning

confidence: 99%

Modeling lung cell development using human pluripotent stem cells

Ngan

Quach

Dierolf

et al. 2021

Preprint

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Human PSC (hPSC) differentiations can capture developmental phenotypes and processes and are useful for studying fundamental biological mechanisms driving tissue morphogenesis and cell lineage development. Here, we show for the first time the temporal development of lung cell lineages using hPSC that model developmental milestones observed in primary tissue, the generation of renewable fetal lung epithelial organoids, and the functional utility of the lung models at different differentiation stages for Cystic fibrosis disease modeling. We first show the presence of hPSC-derived lung progenitor cells reminiscent of early trimester lung development and can capture a population enriched with basal stem cells that generates renewable airway organoids. Maturation and polarization in air liquid interface (ALI) generates additional epithelial cell lineages found in adult lung tissues including pulmonary neuroendocrine, brush, mature basal, ciliated and secretory cell types. Finally, pseudotime analysis of the integrated datasets from the fetal and ALI stages reveal the developmental trajectories of the cells as they emerge during differentiation. Overall, hPSC differentiation can capture aspects of human lung development and potentially provide important insight into congenital causes of diseases.

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“…Cholesterol accessibility (or chemical activity) is further modulated by sphingolipids, which sequester cholesterol in complexes thereby blocking Hh transduction ( Kinnebrew et al, 2019 ). Besides Hh, sphingolipids regulate several other cilia-based pathways including GPCRs (reviewed in Kaiser et al, 2020 ). More studies are needed to further evaluate the effects of lipid dynamics on ciliary signaling.…”

Section: Lipids In Cilia-based Signalingmentioning

confidence: 99%

The Enigmatic Role of Lipids in Cilia Signaling

Nechipurenko

2020

Front. Cell Dev. Biol.

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Primary cilia are specialized cellular structures that project from the surface of most cell types in metazoans and mediate transduction of major signaling pathways. The ciliary membrane is contiguous with the plasma membrane, yet it exhibits distinct protein and lipid composition, which is essential for ciliary function. Diffusion barriers at the base of a cilium are responsible for establishing unique molecular composition of this organelle. Although considerable progress has been made in identifying mechanisms of ciliary protein trafficking in and out of cilia, it remains largely unknown how the distinct lipid identity of the ciliary membrane is achieved. In this mini review, I summarize recent developments in characterizing lipid composition and organization of the ciliary membrane and discuss the emerging roles of lipids in modulating activity of ciliary signaling components including ion channels and G protein-coupled receptors.

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Sphingolipids controlling ciliary and microvillar function

Cited by 17 publications

References 144 publications

Role of Lipids in Morphogenesis of T-Cell Microvilli

Role of Lipids in Morphogenesis of T-Cell Microvilli

Modeling lung cell development using human pluripotent stem cells

The Enigmatic Role of Lipids in Cilia Signaling

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