Differential interference contrast tomography

Abstract

We present a new approach to optical tomography of phase objects that is referred to as differential interference contrast tomography (DICT). The main feature of DICT is that the result of tomographic reconstruction is a 3D DIC image. This image is described by partial derivative of 3D refractive index distribution in one direction. The DICT setup consists of a lateral shearing phase-shifting interference microscope with low-coherent LED illumination. To create projections of the sample at various illumination angles, an angular scanning beam was used. 3D DIC tomograms of a white blood cell are presented. The comparison between the reconstructed DIC tomogram slices and the conventional DIC images of the same sample at the same depths are also represented.

3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy

“…\end{equation}$$If we now estimate the difference between $$\mathcal {H}_{90}(x,y)$$ and $$\mathcal {H}_{0}(x,y)$$, we obtain: $$$$\begin{align} \mathcal {H}_{90}{\left(x,y\right)}&-\mathcal {H}_{0}{\left(x,y\right)}= \mathcal {H}_{90}{\left(x,y\right)}-\nonumber \\ &\mathcal {H}_{90}{\left(x+\Delta x,y+\Delta y\right)}\exp {\left(i\frac{\pi }{2}\right)}. \end{align}$$$$One can notice that Equation () is nothing else that the phase‐shifted gradient of $$\mathcal {H}_{90}(x,y)$$, which is the formal equivalent of a DIC image 33–36 …”

“…One can notice that Equation ( 2) is nothing else that the phase-shifted gradient of  90 (𝑥, 𝑦), which is the formal equivalent of a DIC image. [33][34][35][36] Experimental proof of concept is proposed in Figure 3. Here, a slice of the TDM reconstructed data is proposed.…”

“…Therefore, we can write: If we now estimate the difference between and , we obtain: One can notice that Equation ( 2 ) is nothing else that the phase‐shifted gradient of , which is the formal equivalent of a DIC image. 33 , 34 , 35 , 36 …”

Synthetic aperture gradient light interference microscopy (SA-GLIM) for high throughput label-free imaging

“…2a). Because SAIL employs common-path interferometry, where the two interference beams travel in the same direction, the resulting phase measurement does not contain the phase ramp associated with the illumination direction [50,64]. To reverse this process, for each measurement at an oblique illumination angle, the complex image field is multiplied by exp( ) i i   kr to shift the image spectrum with respect to the illumination direction.…”

Transmission Structured Illumination Microscopy for Quantitative Phase and Scattering Imaging

“…Quantitative phase microscopy (QPM) utilizing the phase information of the object wave can provide not only phase-contrast images but also quantitative information about the threedimensional morphology and refractive index distribution of the samples [1][2][3][4][5][6][7][8]. Recently, a more compact module, nominated as quadriwave lateral shearing interferometry (QWLSI), was proved for quantitative phase imaging with one-shot.…”