Differential inhibitory effects of various herb extracts on the activities of reverse transcriptase and various deoxyribonucleic acid(DNA) polymerases.

Ono, Katsuhiko; Nakane, Hideo; Meng, Zeng-Mu; Ose, Youki; Sakai, Yoshimichi; Mizuno, Mizuo

doi:10.1248/cpb.37.1810

Cited by 47 publications

(23 citation statements)

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“…It has been reported that crude extracts of Millettia pachycarpa inhibit DNA polymerase activity in vitro. 16) In our experiment using yeast, none of extracted compounds except wighteone from the same plant interfered with yeast cell growth, thus also with the DNA replication which is essential for growth. Because DNA recognition by RNA polymerase is also critical for cell growth, the antagonism of prenylated isoflavons would not be due to the inhibition of general transcriptional machinery.…”

Section: E2-non-competitive Er Inhibition Of Prenylated Isoflavonesmentioning

confidence: 60%

Anti-Estrogenic Activity of Prenylated Isoflavones from Millettia pachycarpa: Implications for Pharmacophores and Unique Mechanisms

Okamoto

Suzuki

Ueda

et al. 2006

JOURNAL OF HEALTH SCIENCE

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Phytoestrogens containing isoflavonoids are thought to exhibit preventative effects on estrogen-responsive diseases. Chemical modifications, such as prenylation, in biosynthetic processes enhance the structural variety of isoflavonoids and prompted us to carry out a structure-activity relationship study. We determined the estrogenic/anti-estrogenic activities and estrogen receptor (ER)-binding affinities of eight kinds of prenylated isoflavones isolated from Millettia pachycarpa (Leguminosae), and those of two kinds of non-prenylated compounds (genistein and daidzein). By comparing these compounds, the pharmacophores for estrogenic/anti-estrogenic activities were elucidated. None of the tested compounds (except genistein) were estrogenic on ligand-dependent yeast-two hybrid assay. On the other hand, 5 isoflavones showed distinct anti-estrogenic activity. Unexpectedly, the most potent antagonists, isoerysenegalensein E and 6,8-diprenylorobol, showed anti-estrogenic activity comparable to that of 4-hydroxytamoxifen, a typical ER antagonist. This suggests that genistein became an antagonist after prenylation and hydroxylation. The pharmacophores providing genistein with strong antiestrogenic activity were as follows: prenyl groups of the 6-and 8-positions on the A-ring, hydroxyl group of the 6-prenyl moiety or the B-ring (catechol form), non-cyclization of the prenyl group with the A-ring, and non-hydroxylation of the 8-prenyl group on the A-ring. The ER-binding affinities of the isoflavonoids were not sufficiently high to explain their potent antagonistic activities, thus suggesting 17␤-estradiol-noncompetitive mechanisms.

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Section: E2-non-competitive Er Inhibition Of Prenylated Isoflavonesmentioning

confidence: 60%

Anti-Estrogenic Activity of Prenylated Isoflavones from Millettia pachycarpa: Implications for Pharmacophores and Unique Mechanisms

Okamoto

Suzuki

Ueda

et al. 2006

JOURNAL OF HEALTH SCIENCE

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“…Genistein is also known as specific inhibitor of tyrosine-specific protein kinase (Akiyama et al, 1987). Through in vitro experiment it has been proven that crude extracts of M. pachycarpa inhibit DNA polymerase activity (Ono and Nakane, 1989). Okamoto et al (2006) isolated eight chemicals from M. pachycarpa namely, genistein, daidzein, wighteone, alpinumisoflavone, warangalone, auriculasin, 6,8-diprenylorobol, furowanin A, isoerysenegalensein E, and erysenegalensein E. All of these except genistein showed antiestrogenic activity, whereas genistein showed estrogenic activity on ligand-dependent yeast-two hybrid assay.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural observations on tegumental surface of Raillietina echinobothrida and its alterations caused by root‐peel extract of Millettia pachycarpa

Roy

Dasgupta

Tandon

2008

Microscopy Res & Technique

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Millettia pachycarpa Benth (Leguminosae) has a usage in traditional medicine system practiced among the Lushai tribes of Mizoram, a state in North East India, who customarily consume the aqueous extract of the root peel of the plant to get rid of intestinal worm infections. The crude ethanol, methanol, and acetone fractions of the plant were assayed against Raillietina echinobothrida, the intestinal cestode parasite of domestic fowl, to authenticate the putative anthelmintic efficacy and cestocidal potential in particular of the plants. In vitro exposure of the worm to the extract at a concentration of 25 mg/mL phosphate buffered saline (at 37 degrees C +/- 1 degrees C) revealed distortion and disruption of mitochondria, nucleus, nucleolus, nuclear membrane, basal lamina, and tegumental vacuolization in the distal cytoplasm leading to scar formation in the surface. The possible use of the plant as a potential anthelmintic against cestode parasite is discussed.

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“…Prescribed as an anti-cancer drug, Selaginaella is reported to inhibit human DNA polymerase [67]. Consistent with this, it can cause severe bone marrow suppression [71].…”

Section: Selaginaella Doederleinii (She Shang Bai)mentioning

confidence: 72%

Complications of traditional Chinese/herbal medicines (TCM)—a guide for perplexed oncologists and other cancer caregivers

Chiu

Yau

Epstein

2008

Support Care Cancer

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Differential inhibitory effects of various herb extracts on the activities of reverse transcriptase and various deoxyribonucleic acid(DNA) polymerases.

Cited by 47 publications

References 0 publications

Anti-Estrogenic Activity of Prenylated Isoflavones from Millettia pachycarpa: Implications for Pharmacophores and Unique Mechanisms

Anti-Estrogenic Activity of Prenylated Isoflavones from Millettia pachycarpa: Implications for Pharmacophores and Unique Mechanisms

Ultrastructural observations on tegumental surface of Raillietina echinobothrida and its alterations caused by root‐peel extract of Millettia pachycarpa

Complications of traditional Chinese/herbal medicines (TCM)—a guide for perplexed oncologists and other cancer caregivers

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