Differential Gene Expression Pattern between Normal Human Trophoblast and Choriocarcinoma Cell Lines: Downregulation of Heat Shock Protein-27 in Choriocarcinoma in Vitro and in Vivo

Végh, György; Fülöp, Vilmos; Liu, Yan; Ng, Sook Kien; Tuncer, Z S; Genest, David R.; Päldi‐Haris, Piroska; Földi, J.; Mok, Samuel C.; Berkowitz, Ross S.

doi:10.1006/gyno.1999.5599

Cited by 28 publications

(13 citation statements)

References 16 publications

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“…In the rat, HSPB1 was strongly expressed in trophoblast giant cells beginning at Day 11 of gestation [42]. In human pregnancies, HSP27 (HSPB1 in mouse) protein has been detected in the decidua and in the placenta in the intermediate trophoblast and syncytiotrophoblast cells during the first two trimesters [43], and has been suggested to play a role in trophoblast differentiation [44]. HSP27 is more commonly found in placenta from patients with severe preeclampsia compared with that from normal term gestations [45].…”

Section: Discussionmentioning

confidence: 99%

Heat Shock Protein 1 and the Mitogen-Activated Protein Kinase 14 Pathway Are Important for Mouse Trophoblast Stem Cell Differentiation1

Winger¹,

Guttormsen²,

Gavin³

et al. 2007

Biology of Reproduction

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Differentiation of trophoblast cells is a critical process for the proper establishment of the placenta and is, therefore, necessary to maintain embryonic development. Trophoblast stem (TS) cells grown in culture can differentiate into different trophoblast subtypes in vitro mimicking normal trophoblast cell differentiation. Therefore, TS cells are a valuable model system that can be used to elucidate genetic factors that regulate trophoblast cell differentiation. Several transcription factors, when analyzed by targeted gene mutation in mice, have resulted in embryonic lethality due to placental defects and, more specifically, defects of the trophoblast lineages. These studies have helped improve our knowledge about trophoblast cell differentiation, but much is still unknown about the specific mechanisms involved. This study uses TS cell culture to detect proteins with differential expression in proliferating and differentiating TS cells in order to identify proteins with potential roles in the differentiation process. We identified four proteins with differential expression: dimethylarginine dimethylaminohydrolase1 (DDAH1), keratin 8, keratin 18, and HSPB1 (also known as heat shock protein 25, HSP25). Further investigation confirmed the presence of HSPB1 protein during in vitro TS cell differentiation. In addition, we confirmed that phosphorylation of HSPB1 and MAP kinase-activated protein kinase 2 (MAPKAPK2) increased in TS cells during differentiation. Inhibition of MAPK14 (also known as p38 MAPK) resulted in a reduction of HSPB1 phosphorylation and an increase in cell death during TS cell differentiation. These results suggest that HSPB1 and the MAPK14 pathway are important during TS cell differentiation.

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Section: Discussionmentioning

confidence: 99%

Heat Shock Protein 1 and the Mitogen-Activated Protein Kinase 14 Pathway Are Important for Mouse Trophoblast Stem Cell Differentiation1

Winger¹,

Guttormsen²,

Gavin³

et al. 2007

Biology of Reproduction

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“…Decreased expression of heat shock protein 27 was also seen in CCA cell lines in our array study, which agrees with previous findings using a placenta cell line as control. 16 Caspase 10, an initiator of death receptor signalling, is closely related to the sequence of caspase 8. 17 The genes for these two molecules map to the same region of chromosome 2q33-34, so that they might arise from the duplication of one ancestral gene.…”

Section: Discussionmentioning

confidence: 99%

Caspase activity is downregulated in choriocarcinoma: a cDNA array differential expression study

Fong

Xue²,

Ngan³

et al. 2006

J Clin Pathol

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Background: Placental trophoblast can be considered to be pseudomalignant tissue and the pathogenesis of gestational trophoblastic diseases remains to be clarified. Aims: To examine the role of caspases 8 and 10, identified by differential expression, on trophoblast tumorigenesis. Methods: cDNA array hybridisation was used to compare gene expression profiles in choriocarcinoma cell lines (JAR, JEG, and BeWo) and normal first trimester human placentas, followed by confirmation with quantitative real time polymerase chain reaction and immunohistochemistry. Caspase 10 and its closely related family member caspase 8 were analysed. Results: Downregulation of caspase 10 in choriocarcinoma was detected by both Atlas TM human cDNA expression array and Atlas TM human 1.2 array. Caspase 10 mRNA expression was significantly lower in hydatidiform mole (p = 0.035) and chorioarcinoma (p = 0.002) compared with normal placenta. The caspase 8 and 10 proteins were expressed predominantly in the cytotrophoblast and syncytiotrophoblast, respectively, with significantly lower expression in choriocarcinomas than other trophoblastic tissues (p , 0.05). Immunoreactivity for both caspase 8 and 10 correlated with the apoptotic index previously assessed by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (p = 0.02 and p = 0.04, respectively) and M30 (p , 0.001 and p = 0.003, respectively) approaches. Conclusions: These results suggest that the downregulation of capases 8 and 10 might contribute to the pathogenesis of choriocarcinoma.

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“…The placenta contains different trophoblast types with specialized functions [3], and trophoblast cell lines can only partly reflect this complexity. Functional and phenotypic differences between trophoblast cell lines and primary trophoblasts have been extensively studied [38][39][40][41][42][43].…”

Section: Multivariate Analysis Of Tlr Gene Expression and Cytokine Rementioning

confidence: 99%

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

et al. 2015

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Differential Gene Expression Pattern between Normal Human Trophoblast and Choriocarcinoma Cell Lines: Downregulation of Heat Shock Protein-27 in Choriocarcinoma in Vitro and in Vivo

Cited by 28 publications

References 16 publications

Heat Shock Protein 1 and the Mitogen-Activated Protein Kinase 14 Pathway Are Important for Mouse Trophoblast Stem Cell Differentiation1

Heat Shock Protein 1 and the Mitogen-Activated Protein Kinase 14 Pathway Are Important for Mouse Trophoblast Stem Cell Differentiation1

Caspase activity is downregulated in choriocarcinoma: a cDNA array differential expression study

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

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