Differential gene expression levels, chemical profiles, and biological activities of Pueraria candollei var. mirifica callus cultures at different growth stages

Rani, Dolly; Kobtrakul, Khwanlada; Luckanagul, Jittima Amie; Thaweesest, Wuttinont; Rojsitthisak, Pornchai; De-Eknamkul, Wanchai; Vimolmangkang, Sornkanok

doi:10.1007/s11240-021-02105-3

Cited by 8 publications

(3 citation statements)

References 33 publications

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“…IFS2 expression increased significantly in soybean under UV-B radiation, promoting the synthesis of genistein and daidzein, and improving the defense ability of soybean ( 30 ). The contents of daidzein and genistein in callus culture of Pueraria lobata were also closely related to the activity of IFS for isoflavone synthesis ( 31 ). Interestingly, results showed that IF2 expression up-regulated in both hypocotyl and cotyledon calluses, but only daidzein was higher in content in soybean cotyledon callus, while genistein was not detected ( Figure 4F ; Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

et al. 2023

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Isoflavones are a class of flavonoids that belong to a large family of polyphenols and synthesized predominantly in legume, and they play important roles including acting as antioxidant, preventing osteoporosis, reducing the risk of atherosclerosis, and protecting against cardiovascular disease. This study focused on the accumulation and synthetic metabolism of isoflavone in soybean hypocotyl and cotyledon calluses under UV-B radiation. The results showed that UV-B radiation significantly up-regulated the gene expression of phenylalanine ammonia lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), chalcone ketone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS), and enhanced their activity in soybean hypocotyl and cotyledon calluses. As a result, isoflavones content increased by 21.23 and 21.75% in soybean hypocotyl and cotyledon calluses, respectively. Among the isoflavones produced, malonyldaidzin was the dominant one in hypocotyl callus, while malonylglycitin and daidzein were the main isoflavones in cotyledon calluses. This study revealed that UV-B radiation induced isoflavone accumulation in soybean calluses, which could be an efficient strategy to improve the nutritional value of food and produce high levels of bioactive secondary metabolites.

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Section: Discussionmentioning

confidence: 99%

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

et al. 2023

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“…The importance of the accumulation of phenolics is further supported by the detection/increased accumulation of the 55 kDa hypothetical protein/81E8-like cytochrome P450 monooxygenase in desiccated samples. 81E subfamily cytochrome p450 enzymes operate in the specific hydroxylation of phenylpropanoid isoflavones [ 61 , 62 ]. Since among H. rhodopensis metabolites, among others, 2′-hydroxyflavanone, 7-hydroxyflavanone, 4′-methoxyflavanone, 5-methoxyflavanone, and hispiduline (monomethoxyflavone) glycosides are abundant compounds [ 63 , 64 ], the biosynthesis and accumulation of hydroxylated (iso)flavone derivatives indicate their role is desiccation tolerance.…”

Section: Discussionmentioning

confidence: 99%

Protein Changes in Shade and Sun Haberlea rhodopensis Leaves during Dehydration at Optimal and Low Temperatures

Mihailova

Solti

Sárvári

et al. 2023

Plants

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Haberlea rhodopensis is a unique resurrection plant of high phenotypic plasticity, colonizing both shady habitats and sun-exposed rock clefts. H. rhodopensis also survives freezing winter temperatures in temperate climates. Although survival in conditions of desiccation and survival in conditions of frost share high morphological and physiological similarities, proteomic changes lying behind these mechanisms are hardly studied. Thus, we aimed to reveal ecotype-level and temperature-dependent variations in the protective mechanisms by applying both targeted and untargeted proteomic approaches. Drought-induced desiccation enhanced superoxide dismutase (SOD) activity, but FeSOD and Cu/ZnSOD-III were significantly better triggered in sun plants. Desiccation resulted in the accumulation of enzymes involved in carbohydrate/phenylpropanoid metabolism (enolase, triosephosphate isomerase, UDP-D-apiose/UDP-D-xylose synthase 2, 81E8-like cytochrome P450 monooxygenase) and protective proteins such as vicinal oxygen chelate metalloenzyme superfamily and early light-induced proteins, dehydrins, and small heat shock proteins, the latter two typically being found in the latest phases of dehydration and being more pronounced in sun plants. Although low temperature and drought stress-induced desiccation trigger similar responses, the natural variation of these responses in shade and sun plants calls for attention to the pre-conditioning/priming effects that have high importance both in the desiccation responses and successful stress recovery.

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“…DICYP is involved in the phenylpropanoid metabolic pathway in Dimocarpus longan SE during the hormone response, which is mainly regulated by miR413. The downstream biosynthesis genes and the isoamyl transferase of CYP45081E are significantly up-regulated in yellow callus at different growth period in Pueraria candollei , indicating the culture period has a significant effect on the chemical characteristics and biological activities of Pueraria candollei callus [ 33 , 34 ]. Song et al (2020) demonstrated that somatic embryogenesis may involve water stress and the development of Lilium pumilum SE under an appropriate conditions of auxin and cytokinin ratio [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination

Zhang,

Zhao

et al. 2023

BMC Genomics

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Background Tree peony (Paeonia sect. Moutan DC.) is a famous flower native to China with high ornamental, medicinal, and oil value. However, the low regeneration rate of callus is one of the main constraints for the establishment of a genetic transformation system in tree peony. By histomorphological observation, transcriptomic analysis and metabolite determination, we investigated the molecular mechanism of somatic embryogenesis after the establishment of a culture system and the induction of somatic embryo(SE) formation. Results We found that SE formation was successfully induced when cotyledons were used as explants. A total of 3185 differentially expressed genes were screened by comparative transcriptomic analysis of embryogenic callus (EC), SE, and non-embryogenic callus (NEC). Compared to NEC, the auxin synthesis-related genes GH3.6 and PCO2 were up-regulated, whereas cytokinin dehydrogenase (CKX6) and CYP450 family genes were down-regulated in somatic embryogenesis. In SE, the auxin content was significantly higher than the cytokinin content. The methyltransferase-related gene S-adenosylmethionine synthase (SAMS) and the flavonoid biosynthesis-related gene (ANS and F3’5’H) were down-regulated in somatic embryogenesis. The determination of flavonoids showed that rhoifolin and hyperoside had the highest content in SE. The results of transcriptome analysis were consistent with the relative expression of 8 candidate genes by quantitative polymerase chain reaction analysis. Conclusion The results revealed that auxin and cytokinin may play a key role in ‘Fengdan’ somatic embryogenesis. The genes related to somatic embryogenesis were revealed, which has partly elucidated the molecular mechanism of somatic embryogenesis in ‘Fengdan’.

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Differential gene expression levels, chemical profiles, and biological activities of Pueraria candollei var. mirifica callus cultures at different growth stages

Cited by 8 publications

References 33 publications

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

Protein Changes in Shade and Sun Haberlea rhodopensis Leaves during Dehydration at Optimal and Low Temperatures

Molecular mechanism of somatic embryogenesis in paeonia ostii ‘Fengdan’ based on transcriptome analysis combined histomorphological observation and metabolite determination

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