Differential gene expression in loblolly pine (Pinus taeda L.) challenged with the fusiform rust fungus, Cronartium quercuum f.sp. fusiforme

Myburg, Henrietta; Morse, Alison M.; Amerson, Henry V.; Kubisiak, Thomas L.; Huber, D. M.; Osborne, Jason A.; García, Saúl Alejandro; Nelson, C. Dana; Davis, John M.; Covert, Sarah F.; Zyl, Leonel M. van

doi:10.1016/j.pmpp.2006.07.002

Cited by 22 publications

(17 citation statements)

References 22 publications

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“…Given this rapid progress, it seems reasonable to conclude that much of the improvement has been due to selection for major gene resistance [3,[30][31][32]. Genomic markers now enable detection of parents that harbor specific Fr genes, so that their progeny deployed in plantation forests can be monitored for durability under field conditions.…”

Section: Discussionmentioning

confidence: 99%

Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine

Quesada

Resende

Muñoz

et al. 2014

Forests

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Fusiform rust resistance can involve gene-for-gene interactions where resistance (Fr) genes in the host interact with corresponding avirulence genes in the pathogen, Cronartium quercuum f.sp. fusiforme (Cqf). Here, we identify trees with Fr genes in a loblolly pine population derived from a complex mating design challenged with two Cqf inocula (one gall and 10 gall mixtures). We used single nucleotide polymorphism (SNP) genotypes at sufficient density to ensure linkage between segregating markers and Fr genes identifying SNPs that explained high proportions of variance in disease incidence using BayesC, that also were significant using Bayesian Association with Missing Data OPEN ACCESSForests 2014, 5 348 (BAMD) software. Two SNPs mapped near Fr1 and generated significant LOD scores in single marker regression analyses for Fr1/fr1 parent 17 as well as four other parents. One SNP mapped near Fr8 and was significant for parent 28. Two SNPs mapped to linkage groups not previously shown to contain Fr genes and were significant for three parents. Parent 2 showed evidence of Fr gene stacking. Our results suggest that it is feasible to identify trees segregating for Fr genes, and to map Fr genes, based on parental analysis of SNPs that cosegregate with disease incidence in designed resistance screening trials.

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Section: Discussionmentioning

confidence: 99%

Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine

Quesada

Resende

Muñoz

et al. 2014

Forests

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“…For our marker work, we relied primarily on random amplified polymorphic DNA (RAPD) markers that were used in all matrix families. RAPD protocols underwent multiple minor modifications over the many years of this work, but refer to Myburg et al [31] for the most recently optimized protocols. Amplified fragment length polymorphism (AFLP) markers were utilized for only a single family × isolate combination.…”

Section: Fr Gene Mappingmentioning

confidence: 99%

Identification of Nine Pathotype-Specific Genes Conferring Resistance to Fusiform Rust in Loblolly Pine (Pinus taeda L.)

Amerson

Nelson

Kubisiak

et al. 2015

Forests

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Nearly two decades of research on the host-pathogen interaction in fusiform rust of loblolly pine is detailed. Results clearly indicate that pathotype-specific genes in the host interacting with pathogen avirulence cause resistance as defined by the non-gall phenotype under favorable environmental conditions for disease development. In particular, nine fusiform rust resistance genes (Fr genes) are described here including the specific methods to determine each and their localization on the reference genetic map of loblolly pine. Understanding how these and other apparent Fr genes in loblolly pine and other rust-susceptible pines impact resistance screening, parental and progeny selection, and family and clonal deployment is an important area in forest genetics research and OPEN ACCESSForests 2015, 6 2740 operational tree breeding. The documentation of these Fr genes is a key piece of information towards gaining that understanding and ultimately improving breeding and deployment strategies.

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“…In tree 10-005, the + (band present) allele of marker J7_470 and the − (band absent) allele of marker AJ4_420 are coupled with each other and the Fr1 resistance allele, while −J7_470 and +AJ4_420 are coupled with each other and the fr1 non-resistance allele (H. Amerson, unpublished data, cited in Myburg et al 2006). Since RAPD markers are dominant markers, it was necessary that one and, preferably, both markers (J7_470, AJ4_420) be homozygous null (uniformly band absent in the slash female) if one or both of the markers were to be successfully used to make allele calls (Fr1 vs. fr1 inherited from the loblolly parent) in the hybrid progeny.…”

Section: Genetic Marker Background and Preliminary Studiesmentioning

confidence: 99%

“…Haploid and diploid samples were treated the same except that RAPD reactions for haploid samples utilized 5 ng of template DNA, while those for diploid samples utilized 7.5 ng of template DNA. RAPD reactions (with noted haploid vs. diploid template differences), PCR conditions/specifications, gel electrophoresis, DNA band visualization, and recording (for our study markers) all followed the protocol of Myburg et al (2006). Markers J7_470 and AJ4_420 were scored as band present vs. absent by visual assessment.…”

Section: Dna Isolation and Rapd Marker Protocolsmentioning

confidence: 99%

Performance of the loblolly pine fusiform rust disease resistance gene (Fr1) in a slashXloblolly pine hybrid family

Huber

Amerson

2011

Tree Genetics & Genomes

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Unexpectedly high levels of field susceptibility to the fusiform rust fungus observed for slashXloblolly hybrid families in the Cooperative Forest Genetics Research Program Pine Hybrid Trials led to several hypotheses concerning causation of the observed susceptibility. One of these hypotheses involved the failure of major resistance genes to appropriately function in this particular hybrid combination. This current work, involving the loblolly pine resistance gene Fr1 and a fusiform rust inoculum avirulent against Fr1 resistance in a greenhouse trial, delineates the investigation of major gene resistance for a particular slashXloblolly hybrid family. In this trial, the Fr1 resistance allele, derived from the heterozygous (Fr1/fr1) loblolly male parent and transferred to hybrid offspring that should have been segregating 1:1 for resistance, was fully penetrant. Likewise, in the pure-species loblolly control, the Fr1 resistance allele was again fully penetrant, and the performances of our hybrid family and the loblolly control family (both of which segregated for Fr1 resistance) were comparable. These results by inductive reasoning refute the hypothesis that major resistance genes are not appropriately functional in a slashXloblolly hybrid background.

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Differential gene expression in loblolly pine (Pinus taeda L.) challenged with the fusiform rust fungus, Cronartium quercuum f.sp. fusiforme

Cited by 22 publications

References 22 publications

Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine

Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine

Identification of Nine Pathotype-Specific Genes Conferring Resistance to Fusiform Rust in Loblolly Pine (Pinus taeda L.)

Performance of the loblolly pine fusiform rust disease resistance gene (Fr1) in a slashXloblolly pine hybrid family

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