Differential gene expression in egg cells and zygotes suggests that the transcriptome is restructed before the first zygotic division in tobacco

Ning, Jue; Peng, Xiongbo; Qu, Lianghuan; Xin, H. P.; Yan, Tingting; Sun, Mingwei

doi:10.1016/j.febslet.2006.02.028

Cited by 73 publications

(93 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, defining the epigenetic profile several days after pollination (DAP) is not sufficient because epigenetic marks can be highly dynamic (Gutié rrez-Marcos et al, 2006;Jahnke and Scholten, 2009) and the marks maintaining imprinting may be different from the primary imprint(s). Although challenging, recent progress in isolating plant gametes and zygotes (Dresselhaus et al, 1999;Engel et al, 2003;Gutié rrez-Marcos et al, 2006;Ning et al, 2006;Hermon et al, 2007;Wuest et al, 2010) should allow a better characterization of the differential epigenetic states of imprinted alleles.…”

Section: Defining Imprinted Loci: Meeting the Standardsmentioning

confidence: 99%

Regulation and Flexibility of Genomic Imprinting during Seed Development

2011

View full text Add to dashboard Cite

Genomic imprinting results in monoallelic gene expression in a parent-of-origin-dependent manner. It is achieved by the differential epigenetic marking of parental alleles. Over the past decade, studies in the model systems Arabidopsis thaliana and maize (Zea mays) have shown a strong correlation between silent or active states with epigenetic marks, such as DNA methylation and histone modifications, but the nature of the primary imprint has not been clearly established for all imprinted genes. Phenotypes and expression patterns of imprinted genes have fueled the perception that genomic imprinting is specific to the endosperm, a seed tissue that does not contribute to the next generation. However, several lines of evidence suggest a potential role for imprinting in the embryo, raising questions as to how imprints are erased and reset from one generation to the next. Imprinting regulation in flowering plants shows striking similarities, but also some important differences, compared with the mechanisms of imprinting described in mammals. For example, some imprinted genes are involved in seed growth and viability in plants, which is similar in mammals, where imprinted gene regulation is essential for embryonic development. However, it seems to be more flexible in plants, as imprinting requirements can be bypassed to allow the development of clonal offspring in apomicts.

show abstract

Section: Defining Imprinted Loci: Meeting the Standardsmentioning

confidence: 99%

Regulation and Flexibility of Genomic Imprinting during Seed Development

2011

View full text Add to dashboard Cite

show abstract

“…Alternatively, direct analyses using isolated gametes or zygotes are possible, because procedures for isolating viable gametes/zygotes have been established in a wide range of plant species, including monocotyledonous and dicotyledonous plants (reviewed in Kranz 1999 andin Okamoto 2011;Yang et al 2014). The use of gametes, zygotes or embryos isolated from flowers of maize, wheat, rice, Arabidopsis and tobacco has enabled researchers to successfully identify genes expressed specifically or preferentially in male gametes, female gametes, or early embryos (Abiko et al 2013b;Anderson et al 2013;Borges et al 2008;Márton et al 2005;Ning et al 2006;Ohnishi et al 2011;Russell et al 2012;Sprunck et al 2005;Steffen et al 2007;Wang et al 2010;Wuest et al 2010;Yang et al 2006), since it has been supposed that genes expressing specifically/preferentially in gametes or early embryos function in reproductive or developmental processes such as gamete differentiation, gamete fusion, and early zygotic development. In addition to transcriptome-based analyses, single-cell-type proteomic approaches have widely been employed to determine the functions of specific cells (Dai and Chen 2012), and, recently, proteins expressing in rice gametes were globally identified (Abiko et al 2013a).…”

mentioning

confidence: 99%

Isolation of gametes from <i>Brachypodium distachyon</i>

Matsumura

Okamoto

2016

Plant Biotechnology

View full text Add to dashboard Cite

Isolated gametes from maize, rice, Arabidopsis, wheat and tobacco have been used for investigations for mechanisms in reproductive or developmental processes, such as gamete differentiation, gamete fusion, and early zygotic development. In addition, the isolated gametes from maize and rice can also be applied for in vitro fertilization to analyze postfertilization events. In the last decade, Brachypodium distachyon (Brachypodium) has emerged as an effective model for wheat, since wheat with its hexaploid nature shows some analytical difficulties. In this study, to take advantages of this new model monocot plant for investigations using isolated gametes, procedures for the isolation of Brachypodium gametes were established as the first step. Ovaries were first harvested from mature and unpollinated Brachypodium flowers. Thereafter, a transverse incision was placed at the bottom region of the ovary, resulting in direct access to the embryo sac, and egg cells, which were released from the dissected ovaries, were isolated. For sperm cell isolation, when pollen grains were immersed in mannitol solution, sperm cells were successfully released from pollen grains.

show abstract

“…The 6 increased relative expression of several genes in the single-celled wheat zygote in comparison 7 to the egg cell indeed indicated a very early ZGA in wheat. Similar assumption was obtained 8 by the comparison of gene expression in tobacco egg cells and one-celled zygotes using 9 cDNA subtraction (Ning et al 2006) and EST sequencing (Zhao et al 2011) approaches. 10 Therefore, Arabidopsis, tobacco and wheat data support that ZGA starts in plants before the 11 first cell division of the zygote.…”

Section: Est Sequencing 12mentioning

confidence: 53%