Identification of genes preferentially expressed in wheat egg cells and zygotes

Domoki, Mónika; Szűcs, Attila; Jäger, Katalin; Bottka, Sándor; Barnabás, Beáta; Fehér, Attila

doi:10.1007/s00299-012-1367-0

Cited by 7 publications

(8 citation statements)

References 32 publications

(33 reference statements)

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“…In addition to Arabidopsis, genetic, microscopic and genomic studies in multiple flowering plants, including maize (Chen et al, 2017;Dresselhaus et al, 1999;Meyer and Scholten, 2007;Okamoto et al, 2005;Sauter et al, 1998;Scholten et al, 2002), wheat ((Domoki et al, 2013;Sprunck et al, 2005)), tobacco (Ning et al, 2006;Zhao et al, 2011;Xin et al, 2011) and rice ((Abiko et al, 2013;Anderson et al, 2013;Anderson et al, 2017;Ohnishi et al, 2014;Ohnishi et al, 2019)), indicate that ZGA occurs soon after fertilization in zygotes. In plants, zygotes mark the transition from the haploid gametophytic to diploid sporophytic phase of the life cycle.…”

Section: Discussionmentioning

confidence: 99%

“…Histological and molecular evidence in Hyacinthus orientalis (hyacinth, (Niedojadło et al, 2012;Pięciński et al, 2008), Nicotiana tabacum (tobacco, (Ning et al, 2006;Zhao et al, 2011), Oryza sativa (rice, (Abiko et al, 2013;Anderson et al, 2013;Anderson et al, 2017;Ohnishi et al, 2014;Ohnishi et al, 2019), Triticum aestivum (wheat, (Domoki et al, 2013;Sprunck et al, 2005) and Zea mays (maize, (Chen et al, 2017;Dresselhaus et al, 1999;Meyer and Scholten, 2007;Okamoto et al, 2005;Sauter et al, 1998) altogether indicate that large-scale transcriptional activities increase in zygotes after fertilization and prior to the first division. These results suggest that, similar to animals, plant zygotic genomes may also transition from a transcriptionally quiescent to active state.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional Activation of Arabidopsis Zygotes Is Required for Their Initial Division

Kao

Nodine

2019

Preprint

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Commonly referred to as the maternal-to-zygotic transition, the shift of developmental control from maternal-to-zygotic genomes is a key event during animal and plant embryogenesis.Together with the degradation of parental gene products, the increased transcriptional activities of the zygotic genome remodels the early embryonic transcriptome during this transition.Although evidence from multiple flowering plants suggests that zygotes become transcriptionally active soon after fertilization, the timing and developmental requirements of zygotic genome activation in Arabidopsis thaliana (Arabidopsis) remained a matter of debate until recently. In this report, we optimized an expansion microscopy technique for robust immunostaining of Arabidopsis ovules and seeds. This enabled the detection of marks indicative of active transcription in zygotes before the first cell division. Moreover, we employed a live-imaging culture system together with transcriptional inhibitors to demonstrate that such active transcription is required in zygotes. Our results indicate that zygotic genome activation occurs soon after fertilization and is physiologically required prior to the initial zygotic division in Arabidopsis. T. (2013) Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up-or down-regulated genes in zygotes after fertilization. Transcriptomes of isolated Oryza sativa gametes characterized by deep sequencing: evidence for distinct sex-dependent chromatin and epigenetic states before fertilization. Plant J., 76, 729-741.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptional Activation of Arabidopsis Zygotes Is Required for Their Initial Division

Kao

Nodine

2019

Preprint

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“…Using microdissection, Sprunck et al (2005) constructed a cDNA library from wheat egg cells, and a total of 404 clusters were found to function in metabolic activity, mRNA translation and protein turnover. Subsequently, another 226 expressed sequence tags (ESTs) were studied in wheat egg cells ( Domoki et al, 2013 ). In a similar analysis carried out in tobacco, thousands of ESTs were detected, which may be involved in a variety of developmental processes ( Ning et al, 2006 ; Zhao et al, 2011 ).…”

Section: Gamete-carried Maternal or Paternal Transcripts Involved In mentioning

confidence: 99%

“…Gametogenesis, fertilization and embryogenesis occur deep in the plant saprophytic tissues, thus rendering it difficult to observe the developmental events and investigate the molecular mechanisms of these processes directly. Modern technological advances have allowed the isolation and analysis of gametes, zygotes, and early embryos in a wide variety of plants including maize, tobacco, Arabidopsis , rice, and wheat ( Engel et al, 2003 ; Zhao et al, 2011 ; Nodine and Bartel, 2012 ; Anderson et al, 2013 ; Domoki et al, 2013 ). Therefore, great advances have been made toward understanding the role of uniparental transcripts in plant embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

The expression and roles of parent-of-origin genes in early embryogenesis of angiosperms

et al. 2014

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Uniparental transcripts during embryogenesis may arise due to gamete delivery during fertilization or genomic imprinting. Such transcripts have been found in a number of plant species and appear critical for the early development of embryo or endosperm in seeds. Although the regulatory expression mechanism and function of these genes in embryogenesis require further elucidation, recent studies suggest stage-specific and highly dynamic features that might be essential for critical developmental events such as zygotic division and cell fate determination during embryogenesis. Here, we summarize the current work in this field and discuss future research directions.

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“…They represent part of the transcriptome in a given tissue and/or at a given developmental stage. As of January 1, 2013, over one million ESTs are available for wheat in GenBank of the National Center for Biotechnology Information (NCBI) and are valuable for identifying genes involved in biotic and abiotic stress [ 18 – 22 ], kernel development and quality [ 23 , 24 ], and development [ 21 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach

Deng

et al. 2018

International Journal of Genomics

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Inflorescence represents the highly specialized plant tissue producing the grains. Although key genes regulating flower initiation and development are conserved, the mechanism regulating fertility is still not well explained. To identify genes and gene network underlying inflorescence morphology and fertility of bread wheat, expressed sequence tags (ESTs) from different tissues were analyzed using a comparative transcriptomics approach. Based on statistical comparison of EST frequencies of individual genes in EST pools representing different tissues and verification with RT-PCR and RNA-seq data, 170 genes of 59 gene sets predominantly expressed in the inflorescence were obtained. Nearly one-third of the gene sets displayed differentiated expression profiles in terms of their subgenome orthologs. The identified genes, most of which were predominantly expressed in anthers, encode proteins involved in wheat floral identity determination, anther and pollen development, pollen-pistil interaction, and others. Particularly, 25 annotated gene sets are associated with pollen wall formation, of which 18 encode enzymes or proteins participating in lipid metabolic pathway, including fatty acid ω-hydroxylation, alkane and fatty alcohol biosynthesis, and glycerophospholipid metabolism. We showed that the comparative transcriptomics approach was effective in identifying genes for reproductive development and found that lipid metabolism was particularly active in wheat anthers.

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Identification of genes preferentially expressed in wheat egg cells and zygotes

Cited by 7 publications

References 32 publications

Transcriptional Activation of Arabidopsis Zygotes Is Required for Their Initial Division

Transcriptional Activation of Arabidopsis Zygotes Is Required for Their Initial Division

The expression and roles of parent-of-origin genes in early embryogenesis of angiosperms

Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach

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