Differential Free Radical Scavenging Activity and Radioprotection of Caesalpinia Digyna Extracts and its Active Constituent

Abstract

Two extracts E1and E2 were prepared from the dried root of the plant Caesalpinia digyna by extracting with solvents of different polarity. The extracts were standardized with respect to a polyphenol, bergenin, by LC- MS analysis and they were subjected to free radical scavenging activity and in vitro radioprotection studies. Free radical reactions were carried out with superoxide, hydroxyl, and peroxyl radicals and DPPH. In vitro radioprotecting activity was studied by following their effect on gamma-radiation induced lipid peroxidation, protein carbonylation and DNA damage. The results indicated that E1 with higher free radical scavenging ability is also a more potent inhibitor of radiation induced damage to proteins, DNA and liposomes than E2. Comparing the results with those for bergenin indicated that bergenin alone is not responsible for the free radical scavenging ability and in vitro radioprotection. The studies also confirmed that the extracts enriched with bergenin are more effective than the isolated polyphenol, bergenin.

Comparative Antioxidant and Antiproliferative Activities of Red and White Pitayas and Their Correlation with Flavonoid and Polyphenol Content

“…This hypothesis is supported by the results of the LC‐MS/MS analysis, which revealed the presence of betanin and its derivatives, flavonol glycosides as well as hydroxycinnamic acid derivatives in the tested pitaya extracts. Moreover, our findings also indicate that the polyphenols present in the tested pitaya extracts play important antioxidant roles, supported by the results of several recent studies (Tachakittirungrod and others 2007; Soo‐Jin and others 2008; Singh and others 2009).…”

Diversity, pharmacology and synthesis of bergenin and its derivatives: Potential materials for therapeutic usages

“…Bergenin (1) itself is a good scavenger of hydroxyl radicals but not so effective in scavenging other free radicals like superoxide radical and DPPH [33,35,41,42,50,52,55,64,110,111,148,188,189]. Significant antioxidant activity of bergenin in hydrogen peroxide, ABTS, DPPH and inhibition of lipid peroxidation assays with IC 50 values 32.54, 75.06, 165.35 and 365.12 μg/mL, respectively, was reported by Srinivasan et al [54].…”

“…It exhibited melanogenesis inhibitory activity in α-melanocyte-stimulating hormone (α-MSH)-stimulated B 16 melanoma cells and therefore may be valuable as the potential skin whitening agents. Bergenin exhibited protection effect in γ-radiation induced DNA (pBR322) damage [55]. No antitumor activity of bergenin on human gastric carcinoma cell line MGC-803 was reported [52].…”

“…were given. Bergenin content in C. digyna roots was estimated by LC-MS and found to be contained nearly 30% in the plant extracts [55].…”

Plant Extracts and Plant-Derived Compounds: Promising Players in Countermeasure Strategy Against Radiological Exposure: A Review

“…(Family: Fabaceae; Subfamily: Caesalpinioideae), a large, scandent, prickly shrub growing wild in the shrub forests of the eastern Himalayas, Nilgiris, Ceylon, Malaya islands etc. have been studied for its free radical scavenging property, and protection from oxidative damages (Srinivasan et al, 2007;Singh et al, 2009). Singh et al (2009) showed that the methanolic root extract (E1; for polar constituents) and acetone root extract (E2; for non-polar constituents), standardized with bergenin (the active constituent of the plant) content is a potent inhibitor of superoxide (O 2…”