Differential expressions of surfactant protein SP-A, SP-B, and SP-C mRNAs in rats with streptozotocin-induced diabetes demonstrated by in situ hybridization.

Sugahara, Kazuhiro; Iyama, Ken Ichi; Sano, Kimihiko; Morioka, Tohru

doi:10.1165/ajrcmb.11.4.7917308

Cited by 28 publications

(25 citation statements)

References 23 publications

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“…Sugahara et al (22) studied SP-A mRNA in streptozotocin-induced diabetic rats and observed increased SP-A mRNA in alveolar type II cells and Clara cells from diabetic lungs compared with those from control lungs. The relative abundance of SP-A mRNA increased approximately twofold in bronchiolar epithelial cells of diabetic lungs above that in controls (23).…”

Section: Ferná Ndez-real and Associatesmentioning

confidence: 77%

Circulating Surfactant Protein A (SP-A), a Marker of Lung Injury, Is Associated With Insulin Resistance

et al. 2008

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OBJECTIVES -Impaired lung function and inflammation have both attracted interest as potentially novel risk factors for glucose intolerance, insulin resistance, and type 2 diabetes. We hypothesized that circulating levels of surfactant protein (SP)-A, which reflects interstitial lung injury, could be associated with altered glucose tolerance and insulin resistance.RESEARCH DESIGN AND METHODS -Circulating SP-A concentration and metabolic variables (including insulin sensitivity by minimal model method, n ϭ 89) were measured in 164 nonsmoking men.RESULTS -Circulating SP-A concentration was significantly higher among patients with glucose intolerance and type 2 diabetes than in subjects with normal glucose tolerance, even after adjustment for BMI, age, and smoking status (ex/never). The most significant differences were found in overweight and obese subjects with altered glucose tolerance (n ϭ 59) who showed significantly increased serum SP-A concentrations (by a mean of 24%) compared with obese subjects with normal glucose tolerance (n ϭ 58) (log SP-A 1.54 Ϯ 0.13 vs. 1.44 Ϯ 0.13; P Ͻ 0.0001). Insulin sensitivity (P ϭ 0.003) contributed independently to 22% of SP-A variance among all subjects. In subjects with altered glucose tolerance, insulin sensitivity (P ϭ 0.01) and fasting triglycerides (P ϭ 0.02) contributed to 37% of SP-A variance. Controlling for serum creatinine or C-reactive protein in these models did not significantly change the results.CONCLUSIONS -Lung-derived SP-A protein was associated with altered glucose tolerance and insulin resistance in 164 nonsmoking men. Diabetes Care 31:958-963, 2008

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Section: Ferná Ndez-real and Associatesmentioning

confidence: 77%

Circulating Surfactant Protein A (SP-A), a Marker of Lung Injury, Is Associated With Insulin Resistance

et al. 2008

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“…In this assay, the highest precision was obtained at a sample dilution rate of 1:20 (data not shown). Normally, SP-D is only present in alveoli [17]. However, when the permeability of the alveoli increases as a result of damage, it appears to leak out into the bloodstream [5,6].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Serum Amyloid A and Surfactant Protein D in Sera for Identification of the Clinical Condition of Horses with Bacterial Pneumonia

Hobo

Niwa

Anzai

2007

The Journal of Veterinary Medical Science

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ABSTRACT. In the present study, the concentrations of serum amyloid A and surfactant protein D in sera were measured to evaluate them for identification of the clinical condition of horses with bacterial pneumonia. The study utilized 185 clinically healthy control thoroughbreds and 9 thoroughbreds for experimental infectious study with S. zooepidemicus. Blood samples were collected from the 185 healthy control thoroughbreds. The 9 thoroughbreds were experimentally infected S. zooepidemicus using an endoscopic injection to a lung lobe and were then observed of clinical conditions. Blood samples were collected before inoculation and on the 1-15th, 22nd, and 29th days after inoculation (follow-up group). The levels of SAA and SP-D in the healthy control thoroughbreds were very low. In the follow-up group, the levels of SAA and SP-D changed in parallel with the horses' clinical condition. The pyrexia observed after bacterial inoculation faded by the 11th day, and the changes in SAA and SP-D occurred simultaneous to disappearance of the clinical signs. Measurement of SAA and SP-D proved useful for monitoring the clinical condition of the horses with bacterial pneumonia. Changes in the SP-D value were preceded by changes in the SAA value. Since the changes in SP-D occurred approximately simultaneous to the changes in the horses' clinical signs, we believe that they reflect the condition of the alveolar membranes. We conclude that measurement of SAA and SP-D in sera is useful for identification of the clinical condition of horses with bacterial pneumonia. KEY WORDS: bacterial pneumonia, clinical condition, equine, serum amyloid A, surfactant protein D.

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“…In the current study, the elevated SP-A protein levels in the lungs of hyperglycemic rats under oxidative stress can reflect their overproduction and protective effects on the alveolar epithelium. Insulin treatment restored alterations, such as the disrupted ultrastructure of AECII, reduced surfactant biosynthesis, and impaired secretion of surfactant in STZ-hyperglycemic rats (Sugahara et al, 1992(Sugahara et al, , 1994. In the present study, on one hand, the effect of chard on SP was evaluated for the first time.…”

Section: Discussionmentioning

confidence: 82%

“…SP-A mRNA transcription was considerably enhanced in the lungs of the STZ-treated diabetic rats, but immunohistochemical labeling of SP-A in AECII was weak compared with the control lungs (Sugahara et al, 1994). SP-C transcription, however, declined in the STZ-treated diabetic rats (Sugahara et al, 1992).…”

Section: Discussionmentioning

confidence: 84%

Chard (Beta vulgarisvar.cicla) extract improved hyperglycemia-induced oxidative stress and surfactant-associated protein alterations in rat lungs

Öztay¹,

Saçan

Kayalar³

et al. 2015

Pharmaceutical Biology

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Context: Chard is used as an antidiabetic agent by the diabetic patients in Turkey. Objective: The effect of chard extract [Beta vulgaris L. var. cicla (Chenopodiaceae)] on the antioxidant system and the expression of surfactant-associated proteins (SP) in the lungs of hyperglycemic rats were examined. Materials and methods: Hyperglycemia was induced by a single dose of streptozotocin (60 mg/kg) provided intraperitoneally. Fourteen days after the rats were rendered hyperglycemic, the chard (2 g/kg/d), insulin (6 U/kg/d), and chard plus insulin (as mentioned above) were administered to rats for 45 d. On day 60, rats' lungs were removed. Oxidative stress parameters and SP expression were assayed. Results: The lungs of hyperglycemic rats were characterized by the induced lipid and protein oxidation, elevated myeloperoxidase and xanthine oxidase activities, decreased glutathione levels, and reduced tissue factor and antioxidant enzymes activities (catalase, superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase). Chard treatment alone and chard treatment combined with insulin were capable of achieving a regression of pulmonary oxidative stress, by inhibiting lipid and protein oxidation, and restoring the antioxidant system of hyperglycemic rats. SP-A expressions were significantly unchanged in all groups, whereas pro-SP-C and SP-D expressions were reduced in hyperglycemic rats. Pro-SP-C and SP-D levels were increased by chard and insulin administrations alone and combined in hyperglycemic rats. Discussion and conclusion: All treatments have a positive effect on the surfactant and antioxidant systems of the lungs of hyperglycemic rats. The best therapeutic effect was provided by treatment with chard extract alone in the compensation of hyperglycemic symptoms.

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Differential expressions of surfactant protein SP-A, SP-B, and SP-C mRNAs in rats with streptozotocin-induced diabetes demonstrated by in situ hybridization.

Cited by 28 publications

References 23 publications

Circulating Surfactant Protein A (SP-A), a Marker of Lung Injury, Is Associated With Insulin Resistance

Circulating Surfactant Protein A (SP-A), a Marker of Lung Injury, Is Associated With Insulin Resistance

Evaluation of Serum Amyloid A and Surfactant Protein D in Sera for Identification of the Clinical Condition of Horses with Bacterial Pneumonia

Chard (Beta vulgarisvar.cicla) extract improved hyperglycemia-induced oxidative stress and surfactant-associated protein alterations in rat lungs

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