Differential expression within a family of novel wound-induced genes in potato

Stanford, Anne; Bevan, Michael W.; Northcote, D. H.

doi:10.1007/bf00339718

Cited by 181 publications

(145 citation statements)

References 37 publications

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“…Comparison of several features of PR-4 proteins including prohevein, the intracellular 20 kDa protein CPB20 from tobacco [11], the extracellular proteins PR4a from tobacco [8,9] and PR2 from tomato [8], the wound-induced proteins from potato [12], and the extracellular PR-4 type protein from barley grain barwin [10]. Gaps introduced to optimize the alignment of the C-terminal sequences of the proteins are indicated by hyphens.…”

Section: Resultsmentioning

confidence: 99%

“…Some of them have additional N-terminal domains homologous with hevein [11,12]. Recently, a novel protein from tobacco leaves, designated CPB20, has been described, which is processed into the mature protein by the removal of an Nterminal signal peptide and a C-terminal propeptide most likely involved in the vacuolar targeting of the protein [11].…”

Section: Introductionmentioning

confidence: 99%

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Processed products of the hevein precursor in the latex of the rubber tree (Hevea brasiliensis)

1995

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The 20 kDa precursor of hevein and its C-terminal 14 kDa domain have been isolated. Sequence analysis of the Cterminal tryptic peptides of these proteins and comparison with the cDNA sequence indicate that they represent mature forms from which a C-terminal propeptide, possibly involved in vacuolar targeting, has been removed. The molar ratio of hevein to the C-terminal domain in the lutoid-body fraction of rubber latex is about 30:1. This indicates that not only the pre-and propeptides but also the 14 kDa domain are removed by proteolysis or other processes in the latex vessel after the processing of hevein has taken place.the hevein domain in this protein is not cleaved off in the mature form.Here we report the isolation of the hevein precursor and its C-terminal domain and present evidence that they are the mature proteins from which a C-terminal propeptide has already been removed. The large molar excess of hevein in the lutoidbody fraction of rubber latex indicates that much proitein turnover occurs in the latex vessels of the rubber tree. Materials and methods

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Processed products of the hevein precursor in the latex of the rubber tree (Hevea brasiliensis)

1995

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“…JET8 encodes a wound-induced or win protein and can be described as a PR-4 type protein. The up-regulatiori of a win transcr-ipt specifically within leaf abscission zone tissue has been previously reported by Stanford, Bevan & Northcote (1989) in potato plants tr-ansfor-med with a gu.s t-cporter gene fused to a win pr-ornoter sequence. This type of peptide can also be assigned to the chitinase group as many of them, like JET8, have a chitin-binding site.…”

Section: Discussionmentioning

confidence: 66%

Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra^*

Coupe

Taylor

Roberts

1997

Plant Cell & Environment

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Differential screening of a cDNA library generated from RNA extracted from etiiylene-treated leallet abscis.sion zones of Sawhucus iiigra resulted in the isolation of 20 abscission-related clones. These clones could be grouped into seven families. Sequencing of members of these families revealed that the majority encoded pathogenesisrelated (PR) proteins, and these could be identified by sequence homology as a polyphenol oxidase (PPO), a PR-1 type protein, a Chial type chitinase, a PR-4 type protein similar to the potato win peptides, a PR-6 type proteinase inhibitor, a Chia4 type chitinase aiid a metallothionein-like protein (Coupe, Taylor & Roberts 1995. Planta 197, 442-447). Northern analysis revealed that these mRNAs were not expressed in freshly excised material but accumulated primarily in the abscission zone tissue after 18 h of exposure to etiiylene at a time when abscission of the leaflet explants bad reached 70%. Expression of the PPO and the Chia4-type ebitinase was ethylene-dependent while that of the PR-4 type was up-regulated in the abscission zone tissue in tbe absence of tbe gas. The characterization of these mRNAs and their encoded proteins is presented and their possible roles during abscission are discussed.

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“…The 45 kD protein shares immunological determinants and partial sequence homology with the N-terminal residues of proteins that bind or hydrolyze chitin. Specifically, homology was found with the cysteine-rich domain of basic chitinases and proteins that bind chitin but do not have chitinase activity, like the wound-induced proteins winl and win2 of potato (23).…”

Section: Discussionmentioning

confidence: 99%

Identification and Kinetics of Accumulation of Proteins Induced by Ethylene in Bean Abscission Zones

Campillo

Lewis²

1992

Plant Physiol.

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A two-dimensional gel electrophoresis system that combines a cationic polyacrylamide gel electrophoresis at pH near neutrality with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the spectrum of basic polypeptides that accumulate in bean (Phaseolus vulgaris) abscission zones after treatment with ethylene. Results showed that, as abscission progressed, at least seven basic proteins accumulated in the abscission zone prior to the accumulation of 9.5 cellulase. Six of the seven proteins correspond to pathogenesis-related (PR) proteins. Among them, two isoforms of fl-1,3-glucanase and multiple isoforms of chitinase were identified. A 22 kilodalton polypeptide that accumulated to high levels was identified as a thaumatinlike protein by analysis of its N-terminal sequence (up to 20 amino acids) and its serological relationship with heterologous thaumatin antibodies. A 15 kilodalton polypeptide serologically related to PR P1 (p14) from tomato was identified as bean PR P1 (p14)-like protein. The kinetics of accumulation of glucanases, chitinases, thaumatin-like and PR P1 (pl4)-like proteins during ethylene treatment were similar and they showed that PR proteins accumulated in abscission zones prior to the increase in 9.5 cellulase. Addition of indoleacetic acid, a potent inhibitor of abscission, reduced the accumulation of these proteins to a similar extent (60%). The synchronized accumulation of this set of PR proteins, early in the abscission process, may play a role in induced resistance to possible fungal attack after a plant part is shed. The seventh protein does not correspond to any previously characterized PR protein. This new 45 kilodalton polypeptide accumulated in abscission zones on exposure to ethylene concomitantly with the increase in 9.5 cellulase. Its N-terminal sequence (up to 15 amino acids) showed some homology with the amino terminal sequence of chitinase. Polyclonal antibodies against chitinase recognized the 45 kilodalton polypeptide, but polyclonal antibodies against the 45 kilodalton protein recognized chitinase weakly. When abscission was inhibited by addition of indoleacetic acid, the accumulation of the 45 kilodalton protein was strongly inhibited (80%). This result suggests that the 45 kilodalton polypeptide may play a more direct role in abscission.known, however, that RNA and protein synthesis are required for abscission to occur (2); after ethylene treatment, abscission zones respond with the induction and accumulation of new proteins (12, 13); and after fracture, the cells of the scar surface, when viewed under scanning electron microscopy, appear rounded as if they were protoplasts (21). Thus, abscission involves the expression, synthesis, and secretion of hydrolytic enzymes in a highly coordinated manner. In bean, a cellulase, referred to as 9.5 cellulase, has been implicated in abscission. This cellulase is induced by ethylene and accumulates to high levels in the abscission zone (25). The increase in activity of 9.5 cellulase has been correlated...

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Differential expression within a family of novel wound-induced genes in potato

Cited by 181 publications

References 37 publications

Processed products of the hevein precursor in the latex of the rubber tree (Hevea brasiliensis)

Processed products of the hevein precursor in the latex of the rubber tree (Hevea brasiliensis)

Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra^*

Identification and Kinetics of Accumulation of Proteins Induced by Ethylene in Bean Abscission Zones

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Differential expression within a family of novel wound-induced genes in potato

Cited by 181 publications

References 37 publications

Processed products of the hevein precursor in the latex of the rubber tree (Hevea brasiliensis)

Processed products of the hevein precursor in the latex of the rubber tree (Hevea brasiliensis)

Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra*

Identification and Kinetics of Accumulation of Proteins Induced by Ethylene in Bean Abscission Zones

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Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra^*