Differential expression proteins associated with bud dormancy release during chilling treatment of tree peony (Paeonia suffruticosa)

Abstract

Endo-dormant flower buds of tree peony must have sufficient chilling duration to reinitiate growth, which is a major obstacle to the forcing culture of tree peony in winter. We used a combination of two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS) to identify the differentially expressed proteins of tree peony after three different chilling treatments: endo-dormancy, endo-dormancy release and eco-dormancy stages. More than 200 highly reproducible protein spots were detected, and 31 differentially expressed spots (P < 0.05) were selected for further analysis. Finally, 20 protein spots were confidently identified from databases, which were annotated and classified into seven functional categories: response to abiotic or biotic stimulus (four), metabolic processes (four), other binding (three), transcription or transcription regulation (two), biological processes (one), cell biogenesis (one) and unclassified (five). The results of qPCR of five genes were mainly consistent with that of the protein accumulation analysis as determined by 2-DE. This indicated that most of these genes were mainly regulated at transcriptional level. The activity of nitrate reductase and pyruvate dehydrogenase E1 was consistent with the 2-DE results. The proteomic profiles indicated activation of citrate cycle, amino acid metabolism, lipid metabolism, energy production, calcium signalling and cell growth processes by chilling fulfilment to facilitate dormancy release in tree peony. Analysis of functions of identified proteins will increase our knowledge of endo-dormancy release in tree peony.

Bud Dormancy in Perennial Fruit Tree Species: A Pivotal Role for Oxidative Cues

“…Several studies showed that the expression of 9- cis -epoxycarotenoid dioxygenases ( NCED ), involved in ABA synthesis, is activated during dormancy induction and maintenance ( Fennell et al, 2015 ; Wang et al, 2016 ; Chao et al, 2017 ; Li et al, 2018 ). In addition, after chilling requirements are satisfied, ABA levels decrease under the control of ABA 8′-hydroxylase (encoded by CYP707A ), which is up-regulated during dormancy release ( Zhang et al, 2015 ; Wang et al, 2016 ; Tuan et al, 2017 ; Li et al, 2018 ). Alternatively, inhibition of active ABA might be related to the production of conjugated forms of ABA that increases in response to cold temperatures: glucose ester of ABA (ABA-GE) in Vitis ( Koussa et al, 1994 ) and an ABA-isomer in cherry ( Götz et al, 2014 ).…”

Identification and characterization of microRNAs in tree peony during chilling induced dormancy release by high-throughput sequencing

“…More recently, Zhang et al . obtained 20 differentially expressed protein spots ( P < 0.05) during dormancy release by combination of two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionisation time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS) 12 . In addition, Zhang et al .…”

Selected Plant Metabolites Involved in Oxidation-Reduction Processes during Bud Dormancy and Ontogenetic Development in Sweet Cherry Buds (Prunus avium L.)

“…Three characteristic phases of dormancy (para-, endo-, and ecodormancy) are discussed. Many studies have been directed to determine the course of these phenophases [ 1 , 2 , 3 , 4 , 5 ], through which a central role for the phytohormone abscisic acid (ABA) has been proposed [ 1 , 3 , 6 ]. The leaf fall (LF) generally marks the end of paradormancy and the onset of endodormancy [ 2 ].…”