Differential Expression Patterns Within the Grapevine Stilbene Synthase Gene Family Revealed Through Their Regulatory Regions

Chialva, Constanza; Muñoz, Claudio; Miccono, María; Eichler, Estefanía; Calderón, Luciano; Prieto, Humberto; Lijavetzky, Diego

doi:10.1007/s11105-018-1073-3

Cited by 9 publications

(8 citation statements)

References 65 publications

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“…These results indicate that VvSTS expression levels in MeJA-treated cells were significantly higher than those in SA-, ABA-or ET-treated cells. This is in agreement with the work of Chialva et al (2018) who observed that the VvSTS genes were significantly induced by MeJA treatment in grapevine suspension cultures. In this study, we thus used 100 μM MeJA as an elicitor for the induction of resveratrol production in the grapevine cell cultures.…”

Section: Expression Analysis Of Stilbene Synthase Genes Following Elisupporting

confidence: 93%

“…Genome-wide analysis of the STS gene family based on the grapevine PN40024 genome revealed the identification of 48 putative STS genes, designated VvSTS1 to VvSTS48, with at least 33 full-length coding genes (Jaillon et al 2007;Vannozzi et al 2012). Expression of VvSTS genes is differentially regulated by biotic and abiotic stresses, pathogen infection, mechanical wounding or hormones (Almagro et al 2014;Chialva et al 2018;Lijavetzky et al 2008;Vannozzi et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Induced extracellular production of stilbenes in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside

Jeong

Park

et al. 2020

Bioresour. Bioprocess.

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We report the high production of stilbenes, including resveratrol and viniferin, in grapevine (Vitis labruscana L.) cell cultures through elicitation with methyl jasmonate (MeJA) and stevioside (STE). Methyl-β-cyclodextrin (MeβCD) is widely used as a solubilizer for resveratrol production. For the first time, we used STE as a solubilizer for stilbene production in plant cell cultures. MeJA was most effective elicitor in activating VvSTS expression and stimulating stilbene biosynthesis in grapevine cell cultures. The maximum concentration of δ-viniferin (892.2 mg/L) production with a small amount of trans-resveratrol (12.2 mg/L) was observed in the culture medium by co-treatment of cells with MeJA and STE, whereas the highest level of trans-resveratrol (371.9 mg/L) with a slight amount of δ-viniferin (11.5 mg/L) was accumulated in the culture medium of cells treated with MeJA and MeβCD. However, neither trans-resveratrol nor δ-viniferin were significantly elevated within the cells by the applications. Notably, predominant production of δ-viniferin and trans-resveratrol was observed in shake and static flask culture medium, respectively, by co-treatment of MeJA and STE. Furthermore, stilbene compounds of resveratrol, ε-viniferin, and δ-viniferin were mainly produced in a 3-L bioreactor culture following elicitation of cells with MeJA and STE. These results provide new strategies for conditional, high-level production of resveratrol and viniferin in cell cultures by utilizing the solubilizing properties of STE or MeβCD.

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Section: Expression Analysis Of Stilbene Synthase Genes Following Elisupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Induced extracellular production of stilbenes in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside

Jeong

Park

et al. 2020

Bioresour. Bioprocess.

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“…In grapevine, at least 33 potentially functional RS genes show high levels of conserved gene structure 54 . Despite the conserved structure, RS grapevine genes have different patterns of transcript accumulation 55 . In peanut, four RS genes catalyze resveratrol synthesis and their up-regulation is positively correlated with an increase in resveratrol content 26 .Thus, the differences found in the RS expression patterns found in this study and previous ones might be due to the analysis of different RS genes.…”

Section: Resultsmentioning

confidence: 99%

Presence of resveratrol in wild Arachis species adds new value to this overlooked genetic resource

Carvalho

Moretzsohn

Brasileiro

et al. 2020

Sci Rep

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Genus Arachis comprises 82 species distributed into nine taxonomic sections. Most Arachis species are wild and those from Arachis section have been evaluated for many traits, since they can be used in peanut breeding. Most of the remaining species have been neglected and understudied. Recently, resveratrol content and expression of a resveratrol synthase gene were analyzed in wild Arachis species. Our aim was to expand the knowledge about resveratrol in Arachis, analyzing species from five sections and evaluating the expression of a resveratrol synthase (RS) gene responsive to ultraviolet light (UV) along the time. In a first experiment, the resveratrol content after UV induction was analyzed on detached leaves of 12 species from five sections. Variation was observed among species and accessions of the same species. the highest contents were found in A. lignosa (843.9 μg/g) and A. triseminata (745.4 μg/g). In a second experiment, RS expression and resveratrol content in four species and one synthetic amphidiploid were analyzed at 0, 7, 15 and 24 h pos induction (hpi) with UV. In most genotypes, the highest RS expression level was at 0 hpi, whereas the highest resveratrol content was at 15 hpi. Our results suggested that resveratrol is ubiquitously present in the genus Arachis with different capacities of synthesis among species and accessions in response to ultraviolet treatment. presence of resveratrol in wild Arachis species adds new value to these genetic resources.

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“…These procedures are relevant because they avoid the technical difficulty and the time needed for whole plant generation, which is a major restriction in Prunus 9,196 . Protocols based on the use of explants such as leaf discs, stem segments, or even whole plants have been achieved in other woody species such as grapevines 197,198 . Nevertheless, these approaches are restricted to a few Prunus species such as P. domestica 199 and P. salicina 200 in experiments that can be considered preliminary assays that later led to stable transformation procedures 201,202 .…”

Section: Development Of Biotechnology Tools: Genetic Transformation Amentioning

confidence: 99%

Prunus genetics and applications after de novo genome sequencing: achievements and prospects

et al. 2019

Self Cite

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Prior to the availability of whole-genome sequences, our understanding of the structural and functional aspects of Prunus tree genomes was limited mostly to molecular genetic mapping of important traits and development of EST resources. With public release of the peach genome and others that followed, significant advances in our knowledge of Prunus genomes and the genetic underpinnings of important traits ensued. In this review, we highlight key achievements in Prunus genetics and breeding driven by the availability of these whole-genome sequences. Within the structural and evolutionary contexts, we summarize: (1) the current status of Prunus whole-genome sequences; (2) preliminary and ongoing work on the sequence structure and diversity of the genomes; (3) the analyses of Prunus genome evolution driven by natural and man-made selection; and (4) provide insight into haploblocking genomes as a means to define genome-scale patterns of evolution that can be leveraged for trait selection in pedigree-based Prunus tree breeding programs worldwide. Functionally, we summarize recent and ongoing work that leverages whole-genome sequences to identify and characterize genes controlling 22 agronomically important Prunus traits. These include phenology, fruit quality, allergens, disease resistance, tree architecture, and self-incompatibility. Translationally, we explore the application of sequence-based marker-assisted breeding technologies and other sequence-guided biotechnological approaches for Prunus crop improvement. Finally, we present the current status of publically available Prunus genomics and genetics data housed mainly in the Genome Database for Rosaceae (GDR) and its updated functionalities for future bioinformatics-based Prunus genetics and genomics inquiry.

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Differential Expression Patterns Within the Grapevine Stilbene Synthase Gene Family Revealed Through Their Regulatory Regions

Cited by 9 publications

References 65 publications

Induced extracellular production of stilbenes in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside

Induced extracellular production of stilbenes in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside

Presence of resveratrol in wild Arachis species adds new value to this overlooked genetic resource

Prunus genetics and applications after de novo genome sequencing: achievements and prospects

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