Differential expression patterns of type I interferon subtypes in mouse embryo fibroblasts: Influence of genotype and viral inducer

Lai, May C.; Yeow, Wen-Shuz; Boyer, S.J.; Beilharz, Manfred W.

doi:10.1016/0166-3542(94)90079-5

Cited by 7 publications

(2 citation statements)

References 19 publications

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“…CEF cells are known to be a good host for virus propagation and replication in vitro . One role of fibroblasts in the early stage of virus infection at least partly involves antigen presentation − and the production of cytokines (e.g., interferons) − as the immediate alarm or barrier against pathogen invasion, whereas viruses utilize their own unique proteins to turn the host into a propagation-friendly environment. From the transcriptome analysis conducted by several laboratories, infection of CEF cells with either the oncogenic (vvMDV) or the HVT strain of MDV revealed unique differential expression of host genes.…”

Section: Discussionmentioning

confidence: 99%

Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek’s Disease Virus

et al. 2012

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Vaccination is an effective strategy to reduce the loss of chickens in the poultry industry caused by Marek's Disease (MD), an avian lymphoproliferative disease. The vaccines currently used are from attenuated serotype 1 Marek's disease virus (MDV) or naturally nononcogenic MDV strains. To prepare for future immunity breaks, functional genomic and proteomic studies have been used to better understand the underlying mechanisms of MDV pathogenicity and the effects induced by the vaccine viruses. In this study, a combined approach of quantitative GeLC-MSE and qualitative ERLIC/IMAC/LC-MS/MS analysis were used to identify abundance changes of proteins and the variations of phosphorylation status resulting from the perturbations due to infection with an attenuated oncogenic virus strain (Md11/75C) and several nononcogenic virus strains (CVI988, FC126 and 301B) in vitro. Using this combined approach, several signal transduction pathways mapped by the identified proteins were found to be altered at both the level of protein abundance and phosphorylation. On the basis of this study, a kinase-dependent pathway to regulate phosphorylation of 4E-BP1 to modulate assembly of the protein translation initiation complex was revealed. The differences of 4E-BP1 phosphorylation patterns as well as the measured abundance changes among several other proteins that regulate host transcriptional and translational activities across the virus strains used in this study provide new insight for future functional and biochemical characterization of specific proteins involved in MDV pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek’s Disease Virus

et al. 2012

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“…NDV was prepared by inoculating an NDV isolate into the allantoic fluid of 9-to 11-day-old embryonated chicken eggs. The allantoic fluid was harvested and clarified 4 days p.i., and NDV titers were assayed as 50% egginfective doses (17). Encephalomyocarditis virus was propagated in L929 cells for the IFN bioassay (17).…”

Section: Methodsmentioning

confidence: 99%

Alpha/beta interferons increase host resistance to murine AIDS

Heng

Price

Lai

et al. 1996

J Virol

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Murine AIDS (MAIDS) is caused by a defective retrovirus present in the LP-BM5 murine leukemia virus mixture. Strains of inbred mice differ in resistance to MAIDS development; some are susceptible (e.g., C57BL/6), while others are resistant (e.g., CBA and B10.BR). As an early block to viral replication in resistant mice has been demonstrated previously by PCR studies, we postulated that alpha/beta interferons (IFN-␣/␤) may be involved in resistance to MAIDS. Susceptible C57BL/6 mice infected with LP-BM5 were treated with IFN-␣/␤ or Newcastle disease virus. Newcastle disease virus induces high endogenous IFN-␣/␤ production in mice. Both treatments delayed the development of MAIDS, as assessed by splenomegaly and T-and B-cell proliferation. In addition, an IFN-␣/␤ response was detected by reverse transcription-PCR and dot blotting 3, 6, and 9 h after LP-BM5 infection in resistant mice but not in susceptible mice. These results suggest that the ability to produce IFN-␣/␤ in response to LP-BM5 infection may contribute to host resistance to MAIDS.

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Transient transfection of mouse fibroblasts with type I interferon transgenes provides various degrees of protection against herpes simplex virus infection

Härle¹,

Cull²,

Guo³

et al. 2002

Antiviral Research

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Differential expression patterns of type I interferon subtypes in mouse embryo fibroblasts: Influence of genotype and viral inducer

Cited by 7 publications

References 19 publications

Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek’s Disease Virus

Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek’s Disease Virus

Alpha/beta interferons increase host resistance to murine AIDS

Transient transfection of mouse fibroblasts with type I interferon transgenes provides various degrees of protection against herpes simplex virus infection

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