We have investigated the plasma proteome by using 2D gel electrophoresis and MS from patients with severe acute respiratory syndrome (SARS). A complete proteomic analysis was performed on four patients with SARS in different time courses, and a total of 38 differential spots were selected for protein identification. Most of the proteins identified are acute phase proteins, and their presence represents the consequence of serial cascades initiated by SARS-coronavirus infection. There are several proteins that have never been identified in plasma before using 2D gel electrophoresis, among which peroxiredoxin II was chosen for further study by analyzing additional 20 plasma samples from patients with probable and suspected SARS and patients with fever, respectively. The results showed that the level of plasma peroxiredoxin II in patients with SARS is significantly high and could be secreted by T cells. Taken together, our findings indicate that active innate immune responses, along with the oxidation-associated injuries, may play a major role in the pathogenesis of SARS.proteomic techniques ͉ acute phase proteins ͉ peroxiredoxin II S evere acute respiratory syndrome (SARS) was first recognized at the end of 2002 in Guangdong, China, and since then the disease has spread to several countries. By late July 2003, Ͼ8,000 SARS cases and Ͼ700 SARS-related deaths were reported from Ͼ25 countries around the world, and no effective treatment has been established so far. Through extensive studies, the causative agent of SARS has been identified as a human SARS-coronavirus (SARS-CoV) (1-3). Although the complete genome sequence of SARS-CoV, the structure of the main protease (3CL protease), and the possible receptor have been determined (4-7), the pathogenesis of SARS is still not fully understood.Plasma proteins are useful targets for diagnostic, prognostic, and͞or therapeutic development. With proteomic tools available recently, profiling of human plasma proteome becomes more feasible in searching for disease-related markers (8). To explore the possible pathogenetic mechanisms involving the progression of SARS, we have analyzed the plasma proteins of 22 different plasma samples from four SARS patients in different time courses by using 2D gel electrophoresis (2DE) in combination with MS. The results showed that most of the plasma proteins found in patients with SARS are acute phase proteins (APPs) generated by inflammatory reactions during SARS-CoV-induced acute lung injuries. Materials and MethodsHuman Subjects. Four SARS patients with a total of 22 plasma samples were selected for complete proteomic analysis. These patients were infected by SARS-CoV through nosocomial transmission during one major SARS outbreak in one municipal hospital in Taipei. For comparison, plasma samples from six healthy individuals were used as controls. The protein concentration of each sample was determined by the standard Bradford method. The use of these samples was approved by the Review Board of the Tri-Service General Hospital, National Def...
a b s t r a c tPlant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic cleft of protein kinases and is therefore least probable to be a target for any modification. This communication illustrates the accessibility of the gatekeeper site (Y670) towards both autophosphorylation and dephosphorylation in the recombinant cytoplasmic domain of symbiosis receptor kinase from Arachis hypogaea (AhSYMRK). Autophosphorylation on gatekeeper tyrosine was detected prior to extraction but never under in vitro conditions. We hypothesize gatekeeper phosphorylation to be associated with synthesis/maturation of AhSYMRK and this phenomenon may be prevalent among RLKs. Structured summary of protein interactions:AhSYMRK and AhSYMRK phosphorylate by protein kinase assay (1,2,3)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.