Differential Expression Patterns of Pleurotus ostreatus Catalase Genes during Developmental Stages and under Heat Stress

Wang, Lining; Wu, Xiangli; Gao, Wei; Zhao, Ming; Zhang, Jinxia; Huang, Chenyang

doi:10.3390/genes8110335

Cited by 42 publications

(47 citation statements)

References 49 publications

(55 reference statements)

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“…Although, the heat shock response has been studied in considerable detail in yeast and plant (McAlister and Finkelstein 1980;Richter et al 2010;Song et al 2012), the mechanism of heat shock response in basidiomycetes remains elusive. Mushrooms evolved various strategies to response and alleviate heat shock stress, including HSPs synthesis, trehalose accumulation, and reactive oxygen species scavenging (Chen et al 2017;Liu et al 2016Liu et al , 2018Liu et al , 2019Wang et al 2017). In this study, we focused on a HSPs gene involved in heat shock response in H. marmoreus.…”

Section: Discussionmentioning

confidence: 99%

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Gao

Guo

et al. 2020

AMB Expr

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The 70-kD heat shock proteins (Hsp70s) have been proved to be important for stress tolerance and protein folding and unfolding in almost all organisms. However, the functions of Hsp70s in mushroom are not well understood. In the present study, a hsp70 gene from Hypsizygus marmoreus, hmhsp70, was cloned and transferred to tobacco (Nicotiana tabacum) to evaluate its function in thermotolerance. Sequence alignments and phylogenetic analysis revealed that HmHsp70 may be located in the mitochondria region. qPCR analysis revealed that the transcription level of hmhsp70 in H. marmoreus mycelia increased after heat shock treatment in high temperature (42 °C) compared with untreated mycelia (at 25 °C). Transgenic tobaccos expressing hmhsp70 gene showed enhanced resistance to lethal temperature compared with the wild type (WT) plants. Nearly 30% of the transgenic tobaccos survived after treated at a high temperature (50 °C and 52 °C for 4 h); however, almost all the WT tobaccos died after treated at 50 °C and no WT tobacco survived after heat shock at 52 °C. This study firstly showed the function of a hsp70 gene from H. marmoreus.

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Section: Discussionmentioning

confidence: 99%

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Gao

Guo

et al. 2020

AMB Expr

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“…The ChamQ SYBR qPCR master mix kit (Vazyme, Nanjing, China) and the ABI 7500 real-time PCR amplifier (Applied Biosystems, Foster City, CA, USA) were used for qPCR. The qPCR amplification procedure was as follows: 95°C for 3 min, 40 cycles at 95°C for 3 s and at 60°C for 32 s, and a final extension at 72°C for 30 s. Relative gene expression was analyzed according to the 2 ϪooCT method (55).…”

Section: Methodsmentioning

confidence: 99%

“…High temperature is one of the abiotic stresses that impact the cultivation of P. ostreatus. To simulate heat stress in growth, the strains were cultured and treated according to our previous methods (55). The mycelia were cultured at 28°C for 5 days, treated at 40°C, and then returned to growth at 28°C.…”

Section: Methodsmentioning

confidence: 99%

“…The expression of ACO protein in the WT and transformed strains was studied using Western blotting with ACO polyclonal antibody as a target. Western blot analysis was performed according to the method of a previous study (55). Briefly, equal amounts of total protein (25 g) were loaded into the lanes, separated in a 10% (wt/vol) sodium dodecyl sulfate PAGE gel, and transferred to polyvinylidene difluoride membranes.…”

Section: Methodsmentioning

confidence: 99%

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Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Hou

Zhao

Huang

et al. 2020

Appl Environ Microbiol

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Pleurotus ostreatus is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO alleviated oxidative damage to P. ostreatus mycelia by inhibiting the protein and gene expression of aconitase (ACO), and additional studies found that the overexpression and interference of aco could affect the content of citric acid (CA). Furthermore, the addition of exogenous CA can induce alternative oxidase (aox) gene expression under heat stress, reduce the content of H 2 O 2 in mycelium, and consequently protect the mycelia under heat stress. An additional analysis focused on the function of the aox gene in the heat stress response of mycelia. The results show that the colony diameter of the aox overexpression (OE-aox) strains was significantly larger than that of the wild-type (WT) strain under heat stress (32°C). In addition, the mycelia of OEaox strains showed significantly enhanced tolerance to H 2 O 2 . In conclusion, this study demonstrates that NO can affect CA accumulation by regulating aco gene and ACO protein expression and that CA can induce aox gene expression and thereby be a response to heat stress. IMPORTANCE Heat stress is one of the abiotic stresses that affect the growth and development of edible fungi. Our previous study found that exogenous NO had a protective effect on mycelia under heat stress. However, its regulatory mechanism had not been elucidated. In this study, we found that NO altered the respiratory pathway of mycelia under heat stress by regulating aco. The results have enhanced our understanding of NO signaling pathways in P. ostreatus.

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“…According to our previous study [68], qPCR was performed to analyze the mRNA expression levels of the aox gene and key antioxidant enzyme genes in the CCMSSC00389 strain and aox-transformed strains subjected to the different treatments. The β-actin and β-tubulin genes were used as internal reference genes, and the relative gene expression was determined according to the 2 −△△CT method.…”

Section: Rna Extraction Reverse Transcription and Qpcrmentioning

confidence: 99%

Alternative Oxidase Gene Induced by Nitric Oxide is Involved in the Regulation of ROS and Enhance the Resistance of Pleurotus Ostreatus to Heat Stress

Hou¹,

Zhao

Huang

et al. 2020

Preprint

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Background: Pleurotus ostreatus is easily affected by temperature during its cultivation. Nitric oxide (NO) plays an important regulatory role in the response to abiotic stress, and previous studies have found that NO can induce alternative oxidase (aox) in response to heat stress (HS) by regulating aconitase. However, the regulatory pathway of NO is complex, and the function and regulation of the aox gene in the response to HS remain unclear.Results: In this study, we found that NO affected the NADH and ATP levels, reduced the H2O2 and O2- contents, and slowed the production of O2-. Further RNA-Seq results showed that NO regulated the oxidation-reduction process and oxidoreductase activity, affected the cellular respiration pathway and activated aox gene expression. The function of aox was determined by constructing overexpression (OE) and RNA interference (RNAi) strains. The results showed that the OE-aox strains showed obvious advantages in experiencing growth recovery after exposure to HS. During exposure to HS, the OE-aox strains exhibited reduced levels of NADH, the product of the TCA cycle, and decreased synthesis of ATP, which reduced the production and accumulation of ROS, whereas the RNAi-aox strains exhibited the opposite result. In addition, aox mediated the expression of antioxidant enzyme genes in the mycelia of P. ostreatus under HS through the retrograde signaling pathway. Conclusions: This study shows that the aox gene in P. ostreatus mycelia can be induced by NO under HS, regulates the TCA cycle and cell respiration to reduce the production of ROS, and can mediate the retrograde signaling pathway involved in the mycelial response to HS.

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Differential Expression Patterns of Pleurotus ostreatus Catalase Genes during Developmental Stages and under Heat Stress

Cited by 42 publications

References 49 publications

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Alternative Oxidase Gene Induced by Nitric Oxide is Involved in the Regulation of ROS and Enhance the Resistance of Pleurotus Ostreatus to Heat Stress

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