Differential expression of zebrafish gpia and gpib during development

Lin, Wen–Jen; Chen, Lihua; Chen, Ming‐Chyuan; Kao, Hsien Ming

doi:10.1016/j.gep.2008.12.006

Cited by 7 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…713 54 scf. 68 casq2 (−/*) XVI 54 dmbx1 (*/*) III 55 I VIII ext1 (*/*) X 56 foxc1 (*/*) III 57 XX VIII gpi (*/*) II 58 foxb1 (*/*) XIX 57 XIX II heyI (*/*) XX 59 hce1 (*/*/*) XVIII 60 scf. 48 X scf.…”

Section: Resultsunclassified

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis

Kaitetzidou

Katsiadaki

Lagnel

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Development requires the implementation of a plethora of molecular mechanisms, involving a large set of genes to ensure proper cell differentiation, morphogenesis of tissues and organs as well as the growth of the organism. Genome duplication and resulting paralogs are considered to provide the raw genetic materials important for new adaptation opportunities and boosting evolutionary innovation. The present study investigated paralogous genes, involved in three-spined stickleback ( Gasterosteus aculeatus ) development. Therefore, the transcriptomes of five early stages comprising developmental leaps were explored. Obtained expression profiles reflected the embryo’s needs at different stages. Early stages, such as the morula stage comprised transcripts mainly involved in energy requirements while later stages were mostly associated with GO terms relevant to organ development and morphogenesis. The generated transcriptome profiles were further explored for differential expression of known and new paralogous genes. Special attention was given to hox genes, with hoxa13a being of particular interest and to pigmentation genes where itgb1 , involved in the melanophore development, displayed a complementary expression pattern throughout studied stages. Knowledge obtained by untangling specific paralogous gene functions during development might not only significantly contribute to the understanding of teleost ontogenesis but might also shed light on paralogous gene evolution.

show abstract

Section: Resultsunclassified

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis

Kaitetzidou

Katsiadaki

Lagnel

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Eif4ebp1 encodes a repressor of translation initiation, and is a target of mTOR ( Wang et al, 2005 ; Dowling et al, 2010 ). The other genes have diverse functions, prc1 is a cell cycle related gene ( Li, Shridhar & Liu, 2003 ), gpi a glycolytic enzyme differentially expressed in zebrafish development ( Lin et al, 2009 ), des2 is involved in sphingolypid synthesis ( Omae et al, 2004 ) whereas the function of mamdc2 is poorly characterized.…”

Section: Resultsmentioning

confidence: 99%

Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs

Guðbrandsson

Franzdóttir

Kristjánsson

et al. 2018

PeerJ

View full text Add to dashboard Cite

Phenotypic differences between closely related taxa or populations can arise through genetic variation or be environmentally induced, leading to altered transcription of genes during development. Comparative developmental studies of closely related species or variable populations within species can help to elucidate the molecular mechanisms related to evolutionary divergence and speciation. Studies of Arctic charr (Salvelinus alpinus) and related salmonids have revealed considerable phenotypic variation among populations and in Arctic charr many cases of extensive variation within lakes (resource polymorphism) have been recorded. One example is the four Arctic charr morphs in the ∼10,000 year old Lake Thingvallavatn, which differ in numerous morphological and life history traits. We set out to investigate the molecular and developmental roots of this polymorphism by studying gene expression in embryos of three of the morphs reared in a common garden set-up. We performed RNA-sequencing, de-novo transcriptome assembly and compared gene expression among morphs during an important timeframe in early development, i.e., preceding the formation of key trophic structures. Expectedly, developmental time was the predominant explanatory variable. As the data were affected by some form of RNA-degradation even though all samples passed quality control testing, an estimate of 3′-bias was the second most common explanatory variable. Importantly, morph, both as an independent variable and as interaction with developmental time, affected the expression of numerous transcripts. Transcripts with morph effect, separated the three morphs at the expression level, with the two benthic morphs being more similar. However, Gene Ontology analyses did not reveal clear functional enrichment of transcripts between groups. Verification via qPCR confirmed differential expression of several genes between the morphs, including regulatory genes such as AT-Rich Interaction Domain 4A (arid4a) and translin (tsn). The data are consistent with a scenario where genetic divergence has contributed to differential expression of multiple genes and systems during early development of these sympatric Arctic charr morphs.

show abstract

“…Overexpression of miR-17-5p downregulated E2F transcription factor 1 (E2F1), and inhibition of miR-17-5p upregulated E2F1 in K562 cells [38]. Glucose energy and metabolism are crucial for embryonic stem cells, induced pluripotent stem cells, and germ cells [11][12][13], and a variety of miRNAs play a role in the regulation of glucose metabolism in the pancreas, liver, brain, muscle, and adipose tissue [8,16]. However, the role of miRNAs, particularly the miR-302 cluster, miR-106, miR-17-5p, and miR-20 cluster, in germ-cell glucose metabolism remains unknown.…”

Section: Regulation Of Glucose Phosphate Isomerase By Mirnamentioning

confidence: 99%

“…In mice, Gpi mRNA expression was increased in embryonic brain, spinal cord, and limb buds [5]. In zebrafish, the gpib isoform was detected specifically in heart, muscle, and eye when compared to the ubiquitous expression of the gpia isoform [8]. Deficiency of GPI is associated with severe hemolytic anemia, whereas its overexpression is associated with Huntington disease in the brain and epithelial-to-mesenchymal transition in tumor cells [6,9,10].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Glucose Phosphate Isomerase by the 3′UTR-Specific miRNAs miR-302b and miR-17-5p in Chicken Primordial Germ Cells1

Rengaraj

Park

Lee

et al. 2013

Biology of Reproduction

View full text Add to dashboard Cite

Glucose phosphate isomerase (GPI) involves in the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate in glucose pathways. Because glucose metabolism is crucial for the proliferation and differentiation of embryonic stem and germ cells, reducing GPI expression may affect the characteristic features of these cells. MicroRNAs (miRNAs) have been shown to regulate genes. In the present study, we investigated the regulation of chicken GPI by its predicted miRNAs. We determined the expression patterns of seven GPI 3'-untranslated region (3'UTR)-targeting miRNAs, including the gga-miR-302 cluster, gga-miR-106, gga-miR-17-5p, and gga-miR-20 cluster in chicken primordial germ cells (PGCs), compared with GPI mRNA. Among the miRNAs, gga-miR-302b, gga-miR-302d, and gga-miR-17-5p were expressed at lower levels than GPI mRNA. The remaining four miRNAs-gga-miR-302c, gga-miR-106, gga-miR-20a, and gga-miR-20b-were expressed at higher levels than the expression of GPI mRNA. Next, we cotransfected four candidate miRNAs-gga-miR-302b, gga-miR-106, gga-miR-17-5p, and gga-miR-20a-with GPI 3'UTR into 293FT cells by dual fluorescence reporter assay. Overexpression of gga-miR-302b and gga-miR-17-5p miRNAs in 293FT cells significantly downregulated GPI expression, whereas the other two miRNAs had no effect. Then, knockdown and overexpression of these four candidate miRNAs were performed by RNA interference assay to regulate GPI in PGCs. In the RNA interference assay, the expression of GPI was greatly regulated by gga-miR-302b and gga-miR-17-5p. Finally, we examined the effects of GPI regulation on PGC proliferation and migration. Our results suggested that the regulation of GPI by gga-miR-302b and gga-miR-17-5p affected PGCs proliferation. However, regulation of GPI using these two miRNAs did not affect the migration of PGCs into embryonic gonads.

show abstract

Differential expression of zebrafish gpia and gpib during development

Cited by 7 publications

References 27 publications

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis

Differential gene expression during early development in recently evolved and sympatric Arctic charr morphs

Regulation of Glucose Phosphate Isomerase by the 3′UTR-Specific miRNAs miR-302b and miR-17-5p in Chicken Primordial Germ Cells1

Contact Info

Product

Resources

About