Differential expression of salivary mucin bacterial aggregating activity with caries status

Slomiany, Bronislaw L.; Piotrowski, J.; Czajkowski, A; Shovlin, Francis; Slomiany, Amalia

doi:10.1016/0020-711x(93)90250-i

Cited by 17 publications

(10 citation statements)

References 21 publications

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“…Early studies of aggregation of oral streptococci by crude high molecular weight fractions of saliva demonstrated association with constituents expressing blood group antigens (79), such as high molecular weight mucins (80). However, subsequent studies with highly enriched preparations of human gel-forming mucins found little bacterial binding or aggregating activities toward oral streptococci (81)(82)(83), similar to our results with murine Muc19.…”

Section: Muc19 Protects Against S Mutans Colonization and Developmensupporting

confidence: 85%

“…Similarly, Raynal et al (12) identified noncovalent association of high density mucins with cystatin, amylase, and GP340. MUC7 and GP340 are large glycoproteins that express blood group antigens (6 -8, 104) and can bind and aggregate S. mutans (81)(82)(83). Therefore, MUC7 and GP340 likely contributed to the aggregating activity of crude high molecular weight fractions of saliva in early studies, as the fractions were prepared under nonreductive and nondissociative conditions.…”

Section: Differences and Similarities In Expression Of Salivary Gelfomentioning

confidence: 99%

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Salivary Mucin 19 Glycoproteins

Culp¹,

Robinson²,

Cash³

et al. 2015

Journal of Biological Chemistry

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Background:We investigated murine salivary Muc19, in vivo and in vitro, to protect against dental infections. Results: Muc19 attenuates dental infections by Streptococcus mutans and promotes its aggregation. Evidence for human salivary MUC19 is presented. Conclusion: Murine Muc19 helps limit colonization via clearance of S. mutans. Significance: A role for Muc19 in clearance expands upon acknowledged innate immune functions of salivary gel-forming mucins.

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Section: Muc19 Protects Against S Mutans Colonization and Developmensupporting

confidence: 85%

Section: Differences and Similarities In Expression Of Salivary Gelfomentioning

confidence: 99%

Salivary Mucin 19 Glycoproteins

Culp¹,

Robinson²,

Cash³

et al. 2015

Journal of Biological Chemistry

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“…It was put forward that fatty acids had been demonstrated in salivary mucous glycoprotein and covalently bound. These fatty acids were ester linked to the nonglycosylated regions of mucous glycoprotein were comprised primary of hexadecanoic, octadecanoic and docosanoic acids 23,24) . But the interaction and variations between lipid and protein components in the saliva, and caries experience have been scarce.…”

Section: Y Tomita N Miyake and S Yamanakamentioning

confidence: 99%

Lipids in Human Parotid Saliva with Regard to Caries Experience

Tomita¹,

Miyake²,

Yamanaka

2008

J. Oleo Sci.

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It has been reported that saliva may play an important role in the prevention and development of enamel caries and that both lipids and protein contents in saliva may be relevant to this role. This study examined the lipid and protein levels in saliva from individuals differing in caries experience. Female subjects (20 to 21 years old) were used divided equally into two groups, caries-susceptible group (CSG) and caries-resistant group (CRG). Stimulated parotid saliva and stimulated whole saliva were collected from the subjects. After centrifugation, each saliva sample was analyzed for the concentrations of lipids and proteins and for the compositions of lipids and fatty acids. The lipid and protein contents in parotid saliva increased in proportion to increase of the flow rate. The lipid content was slightly correlated with the protein one (r = 0.33). Total lipid and protein concentrations were higher in the samples from CSG than those from CRG. The lipid composition was similar in the samples from the two groups; more than half in total lipids was neutral lipids, followed by glycolipids and phospholipids. Neutral lipids and free fatty acid and triacylglyceride concentrations were significantly higher in the samples from CSG than those from CRG (p<0.01 for each). Also stearic, linoleic and docosahexaenoic acids were significantly higher in the former group than the latter one (p<0.05 and p<0.01). In summary, the lipid concentrations in parotid saliva from caries susceptible subjects were higher than those from caries resistant ones, and the difference in fatty acid composition was detected between them. The variations in the lipid levels and fatty acid composition may be associated with those in caries development.

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“…Rosan et al (15) reported a significant increase in S. mutansaggregating activity in the saliva of a caries-resistant group compared to a caries-susceptible group and a significant decrease in S. mutans adhesion-promoting activity in the saliva of the caries-resistant group. Furthermore, Slomiany et al (20) reported that the bacterium-aggregating epitope of salivary protein is expressed to a greater extent in caries-resistant individuals than in caries-susceptible individuals. Therefore, S. mutans aggregation by salivary proteins may be advantageous for dental caries prevention, since it may clear the organisms …”

mentioning

confidence: 99%

Functional Domain of Bovine Milk Lactoferrin Which Inhibits the Adherence of Streptococcus mutans Cells to a Salivary Film

2002

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The bovine lactoferrin molecule and relatively long lactoferrin fragments containing residues 473 to 538 strongly inhibited adherence of Streptococcus mutans to saliva-coated hydroxyapatite beads. Each cysteine residue in Lf411 (residues 473 to 538) was replaced by a serine residue, and the mutants Lf411-C481S and Lf411-C532S strongly inhibited S. mutans adherence. These results suggest that the functional domain of lactoferrin that binds to a salivary film lies in residues 473 to 538 and that the region might be concealed by disulfide bond formation between Cys481 and Cys532 in the Lf411 fragment.Streptococcus mutans has been implicated as the prime cause of dental caries, one of the most common diseases in humans (17,18). Colonization of the tooth surface by S. mutans is initiated by attachment of the organism to salivary components adsorbed on tooth surfaces (7). A 190-kDa S. mutans surface protein antigen, variously designated as antigen I/II, B, IF, P1, SR, or MSL-1 (17), is known to be one of the factors that mediates the binding of the organism (2,7,9).We recently demonstrated that bovine milk lactoferrin inhibits saliva-induced S. mutans aggregation by binding strongly to salivary components and that residues 473 to 538 of the molecule are important in this inhibition (12). There are two types of bacterial interaction with salivary components: salivainduced bacterial aggregation in solution phase and bacterial adherence to salivary components adsorbed on the tooth surface. The mechanisms of these two types of interaction are different (5,14), and therefore, we were unable to conclude that bovine milk lactoferrin inhibits the adherence of bacterial cells to a salivary film.In this study, the effect of bovine milk lactoferrin on adherence of S. mutans to a salivary film was compared with the effects of other milk components. The inhibitory effect of lactoferrin fragments with residues 473 to 538 on S. mutans adherence to a salivary film was also investigated. To study the effect of mutation on S. mutans adherence, we used engineered bovine lactoferrin fragments in which each cysteine residue was substituted by site-directed mutagenesis.Milk components tested for inhibition of S. mutans adherence to saliva-coated hydroxyapatite (S-HA). Unstimulated whole saliva was collected from a single donor (male, 44 years of age) in an ice-chilled tube and clarified by centrifugation. Bovine ␣-casein, ␤-casein, -casein, lactalbumin, lactoferrin, and lactoperoxidase were purchased from Sigma Chemical Co. (St. Louis, Mo.). Bovine ␥-casein was purchased from Research Organics (Cleveland, Ohio), and bovine lactoglobulin was purchased from ICN Biomedicals Inc. (Aurora, Ohio). Bovine immunoglobulin G was prepared from bovine milk, using affinity chromatography on a 5-ml HiTrap protein G column (Amersham Pharmacia Biotech, Uppsala, Sweden) (13).For the adherence assay, 5 mg of spheroidal hydroxyapatite beads (BDH, Poole, England) was incubated with 200 l of clarified whole saliva for 1 h at 37°C and washed three times with bu...

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Differential expression of salivary mucin bacterial aggregating activity with caries status

Cited by 17 publications

References 21 publications

Salivary Mucin 19 Glycoproteins

Salivary Mucin 19 Glycoproteins

Lipids in Human Parotid Saliva with Regard to Caries Experience

Functional Domain of Bovine Milk Lactoferrin Which Inhibits the Adherence of Streptococcus mutans Cells to a Salivary Film

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