Differential expression of salivary glycoproteins in aggressive and chronic periodontitis

Abstract

Objectives The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis. Material and Methods Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies. Results Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin. Conclusions The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.

The potential of salivary biomarkers for predicting the sensitivity and monitoring the response to nonsurgical periodontal therapy: A preliminary assessment

“…In the present study, the level of lactoferrin was significantly higher in the high‐responder subgroup at baseline and decreased significantly after treatment (Table ), with a high OR (6.75) and statistical significance ( P = .003) at baseline with respect to the treatment response (Table ). Several studies have shown high levels of lactoferrin in the gingival crevicular fluid of participants with periodontitis, particularly those with aggressive periodontitis, and these levels correlate with a greater PPD . After periodontal treatment, the local PMN activity is down‐regulated, resulting in a significant reduction in the lactoferrin level .…”

LTF and DEFB1 polymorphisms are associated with susceptibility toward chronic periodontitis development

“…As regards the role of lactoferrin in periodontal disease (Fine, ), higher lactoferrin salivary levels were measured in Australian (Huynh et al., ), Indian (Yadav et al., ), Swedish (Glimvall, Wickstrom, & Jansson, ), Finnish (Suomalainen, Saxen, Vilja, & Tenovuo, ), Taiwanese (Tsai, Kao, & Chen, ; Wei et al., ), American (Fine, Furgang, & Beydouin, ), and British (Adonogianaki, Moughal, & Kinane, ) periodontitis patients with respect to healthy controls, while low lactoferrin level was found among Brazilian (Rocha Dde et al., ) and Chinese (Wu, Shu, Luo, Ge, & Xie, ) periodontitis patients. Finally, among Japanese (Ikuta et al., ), Swedish (Figueredo & Gustafsson, ), and British (Murray, Mooney, & Kinane, ) subjects, no differences between patients with periodontitis and controls were detected.…”

Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis - a cross sectional study

“…According to literature data, degree of periodontal inflammation correlates with cytokines levels 15 . Furthermore, it has been shown that the degree of the periodontal disease is influenced by secretor status 16,17 .…”