Differential expression of recently duplicated PTOX genes in Glycine max during plant development and stress conditions

Maia, Rachel Alves; Saraiva, Kátia Daniella da Cruz; Roque, André Luiz Maia; Thiers, Karine Leitão Lima; Santos, Clesivan Pereira dos; Silva, João Hermínio Martins da; Feijó, Daniel F.; Arnholdt‐Schmitt, Birgit; Costa, José Hélio

doi:10.1007/s10863-019-09810-x

Cited by 4 publications

(3 citation statements)

References 81 publications

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“…Under stress conditions, the PTOX transcripts varied according to stress severity, i.e., PTOX1 mRNA was prevalent under mild or moderate stresses while PTOX2 was predominant in drastic stresses. Overall, the results indicate a functional relevance of this gene duplication in G. max metabolism, whereas PTOX1 could be associated with chloroplast effectiveness and PTOX2 to senescence and/or apoptosis [45].…”

Section: The Differential Expression Patterns and The Complementary Rmentioning

confidence: 75%

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Plastid terminal oxidases in Chlamydomonas: connections with astaxanthin and bio-hydrogen production

Li¹,

Zheng²,

Xiao³

et al. 2020

Preprint

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Background: as a plasto quinol oxidase involved in plastoquinol oxidation in higher plants and microalgae, the plastid terminal oxidase (PTOX) was first recognized in the tomato mutant GHOST (GH) and Arabidopsis mutant IMMUTANS (IM). Genome sequence analysis revealed that duplication of the PTOX gene occurs in certain eukaryotic microalgae, but not in cyanobacteria and most higher plants. PTOX may also be involved in carotenoid synthesis and play a critical protective role against stress, such as high light, heat shock and hyperosmosis. However, the connections of PTOX with astaxanthin and bio-hydrogen production and their functional relationship between two PTOX genes in the model green microalga Chlamydomonas is unknown. Results: we successfully knocked down two ptoxs through RNAi in Chlamydomonas, respectively. We demonstrated that expression levels of both PTOXs were increased under stress conditions, and interestingly when one PTOX was silenced the other’s transcriptional level was significantly raised. Conclusions: this shows a complementary relationship under high light condition. In addition, the astaxanthin accumulation level was up-regulated in silenced ptox2 strain, compared to the wide type strain. What’s more, significantly increased hydrogen production was observed in silenced ptox1 strain. In conclusion, PTOXs in Chlamydomonas are connected with not only astaxanthin accumulation but also hydrogen production, and their knock-down strains provide new insights in manipulating microalgae for high light stress tolerant strains, carotenoid production and even biofuels.

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Section: The Differential Expression Patterns and The Complementary Rmentioning

confidence: 75%

“…In Glycine max, differential expression of recently duplicated PTOX genes during plant development and stress conditions were extensively investigated [45]. The majority of plant species contain only a single gene encodes PTOX.…”

Section: The Differential Expression Patterns and The Complementary Rmentioning

confidence: 99%

Plastid terminal oxidases in Chlamydomonas: connections with astaxanthin and bio-hydrogen production

Li¹,

Zheng²,

Xiao³

et al. 2020

Preprint

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“…With changing environments, the AOX, PTOX and UCP genes show differential expression patterns and are induced by multiple signaling pathways [24][25][26]. Together, AOX, UCP and PTOX are considered primary defense lines mitigating ROS production, an excess of which causes progressive oxidative damage and ultimately cell death.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

Aziz

Germano

Thiers

et al. 2022

Plants

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Plants subjected to stress need to respond rapidly and efficiently to acclimatize and survive. In this paper, we investigated a selected gene set potentially involved in early cell reprogramming in two rice genotypes with contrasting salinity tolerance (Pokkali tolerant and IR29 susceptible) in order to advance knowledge of early molecular mechanisms of rice in dealing with salt stress. Selected genes were evaluated in available transcriptomic data over a short period of 24 h and involved enzymes that avoid ROS formation (AOX, UCP and PTOX), impact ATP production (PFK, ADH and COX) or relate to the antioxidant system. Higher transcript accumulation of AOX (ROS balancing), PFK and ADH (alcohol fermentation) was detected in the tolerant genotype, while the sensitive genotype revealed higher UCP and PTOX transcript levels, indicating a predominant role for early transcription of AOX and fermentation in conferring salt stress tolerance to rice. Antioxidant gene analyses supported higher oxidative stress in IR29, with transcript increases of cytosolic CAT and SOD from all cell compartments (cytoplasm, peroxisome, chloroplast and mitochondria). In contrast, Pokkali increased mRNA levels from the AsA-GSH cycle as cytosolic/mitochondrial DHAR was involved in ascorbate recovery. In addition, these responses occurred from 2 h in IR29 and 10 h in Pokkali, indicating early but ineffective antioxidant activity in the susceptible genotype. Overall, our data suggest that AOX and ADH can play a critical role during early cell reprogramming for improving salt stress tolerance by efficiently controlling ROS formation in mitochondria. We discuss our results in relation to gene engineering and editing approaches to develop salinity-tolerant crops.

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Genome-wide identification of ascorbate-glutathione cycle gene families in soybean (Glycine max) reveals gene duplication events and specificity of gene members linked to development and stress conditions

Costa¹,

Roque²,

Aziz³

et al. 2021

International Journal of Biological Macromolecules

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Differential expression of recently duplicated PTOX genes in Glycine max during plant development and stress conditions

Cited by 4 publications

References 81 publications

Plastid terminal oxidases in Chlamydomonas: connections with astaxanthin and bio-hydrogen production

Plastid terminal oxidases in Chlamydomonas: connections with astaxanthin and bio-hydrogen production

Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

Genome-wide identification of ascorbate-glutathione cycle gene families in soybean (Glycine max) reveals gene duplication events and specificity of gene members linked to development and stress conditions

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