Genome-wide identification of ascorbate-glutathione cycle gene families in soybean (Glycine max) reveals gene duplication events and specificity of gene members linked to development and stress conditions

Costa, José Hélio; Roque, André Luiz Maia; Aziz, Shahid R.; Santos, Clesivan Pereira dos; Germano, Thais Andrade; Batista, Mathias Coelho; Thiers, Karine Leitão Lima; Saraiva, Kátia Daniella da Cruz; Arnholdt‐Schmitt, Birgit

doi:10.1016/j.ijbiomac.2021.07.103

Cited by 12 publications

(6 citation statements)

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“…Recently, our group demonstrated that transcript accumulation of genes linked to early cell reprogramming under stress related primarily to ROS/RNS balancing and energy status connected to cell restructuration and cell cycle regulation [33,[43][44][45][46]. Our approach is in conformity with the view that "optimization of adaptive potential requires reconfiguration of developmental attributes to allow growth adjustment and stress avoidance" [47].…”

Section: Introductionmentioning

confidence: 51%

“…In fact, SOD transcripts in IR29 increased in response to salt stress in all cell compartments, while in Pokkali SOD expression increased only in the chloroplast (Figures 3 and 4), supporting disseminated O 2 − overproduction in IR29. In this context, it is of interest that Costa et al [46] observed an earlier and higher transcript increase of ASC-GSH cycle genes in susceptible soybean genotypes compared to the tolerant ones in response to different biotic and abiotic stresses. S3 and S4.…”

Section: Discussionmentioning

confidence: 98%

“…Also, the ascorbate recycling via ASC-GSH cycle appeared more active in Pokkali because cytosolic/organelles DHAR mRNA levels increased only in this genotype (Figure 3). In this regard, stresstolerant genotypes in Glycine max also differed from susceptible genotypes increasing transcripts involved in ascorbate regeneration [46]. Some works showed that DHAR overexpression successfully conducted transgenic plants to abiotic stress tolerance, such as in response to aluminum and cold [76].…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

Aziz

Germano

Thiers

et al. 2022

Plants

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Plants subjected to stress need to respond rapidly and efficiently to acclimatize and survive. In this paper, we investigated a selected gene set potentially involved in early cell reprogramming in two rice genotypes with contrasting salinity tolerance (Pokkali tolerant and IR29 susceptible) in order to advance knowledge of early molecular mechanisms of rice in dealing with salt stress. Selected genes were evaluated in available transcriptomic data over a short period of 24 h and involved enzymes that avoid ROS formation (AOX, UCP and PTOX), impact ATP production (PFK, ADH and COX) or relate to the antioxidant system. Higher transcript accumulation of AOX (ROS balancing), PFK and ADH (alcohol fermentation) was detected in the tolerant genotype, while the sensitive genotype revealed higher UCP and PTOX transcript levels, indicating a predominant role for early transcription of AOX and fermentation in conferring salt stress tolerance to rice. Antioxidant gene analyses supported higher oxidative stress in IR29, with transcript increases of cytosolic CAT and SOD from all cell compartments (cytoplasm, peroxisome, chloroplast and mitochondria). In contrast, Pokkali increased mRNA levels from the AsA-GSH cycle as cytosolic/mitochondrial DHAR was involved in ascorbate recovery. In addition, these responses occurred from 2 h in IR29 and 10 h in Pokkali, indicating early but ineffective antioxidant activity in the susceptible genotype. Overall, our data suggest that AOX and ADH can play a critical role during early cell reprogramming for improving salt stress tolerance by efficiently controlling ROS formation in mitochondria. We discuss our results in relation to gene engineering and editing approaches to develop salinity-tolerant crops.

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Section: Introductionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

Aziz

Germano

Thiers

et al. 2022

Plants

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“…This system ensures the efficient removal of hydrogen peroxides and lipid peroxides [ 64 ]. Plants exposed to drought stress can accelerate the ASC–GSH cycle to achieve the more efficient detoxification of ROS [ 58 , 65 ], and this is often regulated by abscisic acid, as shown in wheat seedlings exposed to drought [ 66 ]. Our proteomic analysis showed that many DAPs associated with the ASC–GSH cycle are upregulated in C. spinifex roots including NADH and GST.…”

Section: Discussionmentioning

confidence: 99%

Sandbur Drought Tolerance Reflects Phenotypic Plasticity Based on the Accumulation of Sugars, Lipids, and Flavonoid Intermediates and the Scavenging of Reactive Oxygen Species in the Root

Yang

Bai

Wang

et al. 2021

IJMS

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The perennial grass Cenchrus spinifex (common sandbur) is an invasive species that grows in arid and semi-arid regions due to its remarkable phenotypic plasticity, which confers the ability to withstand drought and other forms of abiotic stress. Exploring the molecular mechanisms of drought tolerance in common sandbur could lead to the development of new strategies for the protection of natural and agricultural environments from this weed. To determine the molecular basis of drought tolerance in C. spinifex, we used isobaric tags for relative and absolute quantitation (iTRAQ) to identify proteins differing in abundance between roots growing in normal soil and roots subjected to moderate or severe drought stress. The analysis of these proteins revealed that drought tolerance in C. spinifex primarily reflects the modulation of core physiological activities such as protein synthesis, transport and energy utilization as well as the accumulation of flavonoid intermediates and the scavenging of reactive oxygen species. Accordingly, plants subjected to drought stress accumulated sucrose, fatty acids, and ascorbate, shifted their redox potential (as determined by the NADH/NAD ratio), accumulated flavonoid intermediates at the expense of anthocyanins and lignin, and produced less actin, indicating fundamental reorganization of the cytoskeleton. Our results show that C. spinifex responds to drought stress by coordinating multiple metabolic pathways along with other adaptations. It is likely that the underlying metabolic plasticity of this species plays a key role in its invasive success, particularly in semi-arid and arid environments.

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“…), an annual herb originating from the Yellow River Basin of China, has been cultivated for more than 3000 years 1 , 2 . This plant, which is rich in nutrients, is an important economic crop that also serves as an oil source and is used in the production of high-quality feed for livestock 3 . Soybean is the fourth most cultivated food crop in the world, and in 2019, its production, globally exceeded 300 million tons 4 .…”

Section: Introductionmentioning

confidence: 99%

SNP application improves drought tolerance in soybean

Zhou

Tian

et al. 2023

Sci Rep

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As an important bioactive molecule, nitric oxide (NO) can effectively alleviate the effects of drought stress on crops. However, it is still unclear whether it can increase the stress resistance of soybean. Therefore, in this study, our objective was to explore the effect of exogenous NO application on the physiological characteristics of soybean seedlings under drought stress. As test material, two soybean varieties, HN65 and HN44, were used, while sodium nitroprusside (SNP) of 100 μmol L−1, 200 μmol L−1, 500 μmol L−1, 1000 μmol L−1 served as an exogenous NO donor, and PEG-6000 as an osmotic regulator to simulate drought stress. The effects of irrigation with different SNP concentrations for different days on the physiological characteristics of the soybean seedlings under drought conditions were then investigated. The results obtained showed that the activities of antioxidant enzymes, osmotic regulator contents, as well as the abscisic acid and salicylic acid contents of the plant leaves increased with increasing SNP concentration and treatment time. However, we observed that excessively high SNP concentrations decreased the activities of key nitrogen metabolism enzymes significantly. This study provides a theoretical basis for determining a suitable exogenous NO concentration and application duration. It also highlights strategies for exploring the mechanism by which exogenous NO regulates crop drought resistance.

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Genome-wide identification of ascorbate-glutathione cycle gene families in soybean (Glycine max) reveals gene duplication events and specificity of gene members linked to development and stress conditions

Cited by 12 publications

References 82 publications

Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

Sandbur Drought Tolerance Reflects Phenotypic Plasticity Based on the Accumulation of Sugars, Lipids, and Flavonoid Intermediates and the Scavenging of Reactive Oxygen Species in the Root

SNP application improves drought tolerance in soybean

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