Differential expression of periodontal ligament‐specific markers and osteogenic differentiation in human papilloma virus 16‐immortalized human gingival fibroblasts and periodontal ligament cells

Pi, Sung-Hee; Lee, S.-K.; Hwang, Yu‐Shik; Choi, Mi-Hye; Kim, E.-C.

doi:10.1111/j.1600-0765.2006.00921.x

Cited by 43 publications

(58 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Stock solutions of all chemicals were prepared in distilled water, except 2-aminoethoxydiphenyl borate (2-APB) in ethanol and 4α-phorboldidecanoate (4α-PDD), ionomycin (Iono), Ono, HC, and fura-2/AM in DMSO. (Pi et al 2007). Both human primary and immortalized PDL cells were maintained in α-minimal essential medium (α-MEM; GIBCO, Grand Island, NY, USA) containing 10% FBS with antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin).…”

Section: Reagentsmentioning

confidence: 99%

Hypotonic Stress Induces RANKL via Transient Receptor Potential Melastatin 3 (TRPM3) and Vaniloid 4 (TRPV4) in Human PDL Cells

et al. 2015

View full text Add to dashboard Cite

Bone remodeling occurs in response to various types of mechanical stress. The periodontal ligament (PDL) plays an important role in mechanical stress-mediated alveolar bone remodeling. However, the underlying mechanism at the cellular level has not been extensively studied. In this study, we investigated the effect of shear stress on the expression of bone remodeling factors, including receptor activator of nuclear factor-kappa B (NF-κB) ligand (RANKL) and osteoprotegerin (OPG), as well as its upstream signaling pathway in primary human PDL cells. We applied hypotonic stress to reproduce shear stress to PDL cells. Hypotonic stress induced the messenger RNA (mRNA) and protein expression of RANKL but not OPG. and RANKL mRNA expression. Our study shows that hypotonic stress induced RANKL mRNA expression via TRPM3-and TRPV4-mediated extracellular Ca 2+ influx and RANKL expression. This signaling pathway in PDL cells may play a critical role in mechanical stress-mediated alveolar bone remodeling.

show abstract

Section: Reagentsmentioning

confidence: 99%

Hypotonic Stress Induces RANKL via Transient Receptor Potential Melastatin 3 (TRPM3) and Vaniloid 4 (TRPV4) in Human PDL Cells

et al. 2015

View full text Add to dashboard Cite

show abstract

“…27 The PDLCs were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Gaithersburg, Md) supplemented with 10% fetal bovine serum (FBS) in a humidified atmosphere of 5% CO 2 at 37uC. For differentiation, PDLCs were cultured with differentiation medium (10% FBS/DMEM, including 50 mg/mL ascorbic acid and 10 mM b-glycerophosphate), as described previously.…”

Section: Cell Culturementioning

confidence: 99%

The role of heme oxygenase-1 in mechanical stress- and lipopolysaccharide-induced osteogenic differentiation in human periodontal ligament cells

Cho¹,

Lee²,

Lee³

et al. 2010

The Angle Orthodontist

View full text Add to dashboard Cite

Objective: To investigate the mechanisms through which mechanical stress and lipopolysaccharide treatment modulate osteoblastic differentiation in periodontal ligament cells. Materials and Methods: Cells were treated with lipopolysaccharide and/or mechanical strain applied with a Flexercell Strain Unit. Protein expression and mRNA were analyzed by Western blotting and reverse transcription-polymerase chain reaction, respectively. Results: When lipopolysaccharide was co-applied with mechanical strain, the increase in the expression of bone morphogenetic protein-2, bone morphogenetic protein-7, and Runx2 mRNA seen with mechanical strain alone was restricted, but heme oxygenase-1 expression was further enhanced. Furthermore, pretreatment with an inhibitor of heme oxygenase-1 or inhibitors of p38, mitogen-activated protein kinase, JNK, phosphoinositide 3-kinases, protein kinase G, and nuclear factor kB restricted osteogenic differentiation induced by the application of lipopolysaccharide and mechanical strain. Conclusions: These results suggest that orthodontic force-induced osteogenesis in alveolar bone is inhibited by the accompanying periodontal inflammation via the upregulation of heme oxygenase-1 expression. Thus, the heme oxygenase-1 pathway could provide a possible therapeutic strategy to improve bone formation in orthodontic treatment. (Angle Orthod. 2010;80:740-747.)

show abstract

“…Due to their regenerative potential but limited life span in vitro, PDL-derived cells have been isolated and immortalized independently by several groups ( Fujita et al, 2005;Pi et al, 2007;Fujii et al, 2008;. Our group has recently described the use of a lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) in human MSC.…”

Section: Introductionmentioning

confidence: 99%

Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

Docheva¹,

Padula²,

Popov³

et al. 2010

eCM

View full text Add to dashboard Cite

Periodontal ligament (PDL) can be obtained from patients undergoing orthodontic treatment. PDL contains progenitor cells that can be expanded and differentiated towards several mesenchymal lineages in vitro. Furthermore, PDL-derived cells have been shown to generate bone-and PDL-like structures in vivo. Thus, PDL cells, combined with suitable biomaterials, represent a promising tool for periodontitisrelated research and PDL engineering.Here, a new PDL cell line using lentiviral gene transfer of human telomerase reverse transcriptase (hTERT) was created. HTERT-expressing PDL cells showed similar morphology and population doubling time but an extended lifespan compared to the primary cells. In addition, PDLhTERT cells expressed several characteristic genes and upon osteogenic stimulation produced a calcified matrix in vitro. When cultivated on two topographically different titanium scaffolds (MA and SLA), PDL-hTERT cells exhibited augmented spreading, survival and differentiation on smooth (MA) compared to rough (SLA) surfaces. These findings differ from previously reported osteoblast behaviour, but they are in agreement with the behaviour of chondrocytes and gingival fibroblasts, suggesting a very cell type-specific response to different surface textures.In summary, we report the testing of titanium biomaterials using a new PDL-hTERT cell line and propose this cell line as a useful model system for periodontitis research and development of novel strategies for PDL engineering.

show abstract

Differential expression of periodontal ligament‐specific markers and osteogenic differentiation in human papilloma virus 16‐immortalized human gingival fibroblasts and periodontal ligament cells

Cited by 43 publications

References 36 publications

Hypotonic Stress Induces RANKL via Transient Receptor Potential Melastatin 3 (TRPM3) and Vaniloid 4 (TRPV4) in Human PDL Cells

Hypotonic Stress Induces RANKL via Transient Receptor Potential Melastatin 3 (TRPM3) and Vaniloid 4 (TRPV4) in Human PDL Cells

The role of heme oxygenase-1 in mechanical stress- and lipopolysaccharide-induced osteogenic differentiation in human periodontal ligament cells

Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface

Contact Info

Product

Resources

About